182 research outputs found

    IN VITRO SCREENING OF LOCALLY ISOLATED LACTOBACILLUS SPECIES FOR PROBIOTIC PROPERTIES

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    The present study was conducted to determine the probiotic properties of locally isolated lactobacilli in-vitro conditions. For this purpose, intestinal contents (n=20) were collected from crop, gizzard, ileum and caecum of adult healthy chicks and conventional yogurt samples (n=20) were procured from the local market for the isolation of lactobacilli. These samples were mixed homogeneously in sterilized phosphate buffer saline (PBS) separately. Samples from both sources were inoculated on deMan Rogosa and Sharpe (MRS) agar. L. acidophilus 3, L. rhamnosus and L. salivarius were isolated from intestinal contents, while L. delbrucekii ssp bulgaricus and L. paracasei ssp paracasei 1 were isolated from yogurt samples. These lactobacilli were identified through standard API-50 CHL system and then screened for resistance against bile salt, acidic pH, gastric transit and ability to inhibit pathogens as well as survival under different storage temperatures. Tolerance level was found variable (P<0.05) among all the tested species of lactobacillus. All the tested species, except L. delbrucekii and L. paracasei, showed good survival (P<0.05). All lactobacilli inhibited the growth of E. coli and Staphylococcus aureus, except L. delbrucekii that showed significantly (P<0.05) low antimicrobial effect. The results showed that L. acidophilus 3, L. rhamnosus and L. salivarius fulfilled the criteria of in-vitro screening for probiotic properties

    PREPARATION AND EVALUATION OF VITAMIN E ADJUVANTED OIL EMULSIFIED INFECTIOUS BRONCHITIS EXPERIMENTAL VACCINE

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    The present study was conducted to prepare oil emulsified (OE) infectious bronchitis (IB) experimental vaccines. The vaccines were prepared using the vaccinal strain H-120 Infectious Bonchitis virus (IBV). The virus was cultivated in 9-day old embryonated eggs via allantoic cavity route. Allantoic-amniotic fluid (AAF) was collected and inactivated with formalin @ 0.12%. Water in oil emulsion was prepared by adding one part of AAF to four parts of mineral oil containing water phase (Tween 80) and oil phase (Span 80) surfactants. Hydrophile lypohile balance (HLB) of the emulsion was maintained at 7.0. Two oil emulsified experimental vaccines were prepared. Vaccine-I was prepared without vitamin E and Vaccine-II with vitamin E (300 mg/ml). A total of 120 day-old broiler breeder chickens were divided into 4 groups, A, B, C, and D, each having 30 birds. At the age of 21 days, experimental Vaccine-I, experimental vaccine-II and commercial IB killed (H-120) vaccine were inoculated @ 0.5 ml in the birds of groups A, B and C, respectively. Group D was maintained as nonvaccinated control. Efficacy of the vaccines was evaluated on the basis of humoral immune response (haemagglutination inhibition antibody titres) against IB in the four groups. The seven weeks cumulative mean antibody titres (CMT) of each group were calculated. The highest CMT was observed in group B (130), followed by group C (69), group A (58) and group D (17). Statistical analysis showed that haemagglutination inhibition (HI) antibody titres in group B (vaccine- II) were significantly higher than those of groups A, B and C (P< 0.05)

    A Case of Persistent Methicillin- Resistant Staphylococcus Aureus (MRSA) Bacteraemia with no Identifiable Source: A Peculiar Clinical Challenge

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    AbstractWith the increase in antibiotic resistance, Methicillin-resistant Staphylococcus aureus (MRSA) hasbecome a global health challenge. We present a case of a 74-years-old female who was unwell withcomplaints of dizziness, shortness of breath on exertion and palpitations. She had presented withparoxysmal atrial fibrillation and Methicillin-Resistant Staphylococcus aureus (MRSA) bacteraemiawith concerns for possible endocarditis. Transesophageal Echocardiogram (TEE) performed ruledout any evidence of endocarditis. MRSA sepsis was established based on blood cultures with unclearsources. After 7 days on Vancomycin and Ceftaroline, her repeated blood cultures were still positivefor MRSA, so she was started on IV Cefazolin 2g and IV Daptomycin 6mg/kg every 24 hours. Herblood culture cleared after 5 days of this therapy. Treatment strategies for patients of MRSAB need tobe individualized to achieve infection clearance, while guidelines do serve to provide a basicblueprint of treatment plans for physicians.Keywords: Methicillin-Resistant Staphylococcus Aureus, Bacteremia, Sepsis
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