Can Archival Tissue Reveal Answers to Modern Research Questions?: Computer-Aided Histological Assessment of Neuroblastoma Tumours Collected over 60 Years

Despite neuroblastoma being the most common extracranial solid cancer in childhood, it is still a rare disease. Consequently, the unavailability of tissue for research limits the statistical power of studies. Pathology archives are possible sources of rare tissue, which, if proven to remain consistent over time, could prove useful to research of rare disease types. We applied immunohistochemistry to investigate whether long term storage caused any changes to antigens used diagnostically for neuroblastoma. We constructed and quantitatively assessed a tissue microarray containing neuroblastoma archival material dating between 1950 and 2007. A total of 119 neuroblastoma tissue cores were included spanning 6 decades. Fourteen antibodies were screened across the tissue microarray (TMA). These included seven positive neuroblastoma diagnosis markers (NB84, Chromogranin A, NSE, Ki-67, INI1, Neurofilament Protein, Synaptophysin), two anticipated to be negative (S100A, CD99), and five research antibodies (IL-7, IL-7R, JAK1, JAK3, STAT5). The staining of these antibodies was evaluated using Aperio ImageScope software along with novel pattern recognition and quantification algorithms. This analysis demonstrated that marker signal intensity did not decrease over time and that storage for 60 years had little effect on antigenicity. The construction and assessment of this neuroblastoma TMA has demonstrated the feasibility of using archival samples for research

Different approaches of bibliometric analysis for data analytics applications in non-profit organisations

Aim: Profitable companies that used data analytics have a double gain in cost reduction, demand prediction, and decision-making. However, using data analysis in non-profit organisations (NPOs) can help understand and identify more patterns of donors, volunteers, and anticipated future cash, gifts, and grants. This article presents a bibliometric study of 2673 to discover the use of data analytics in different NPOs and understand its contribution.Methods: We characterise the associations between data analysis techniques and NPOs using, Bibliometrics R tool, a co-term analysis and scientific evolutionary pathways analysis, as well as identify the research topic changes in this field throughout time.Results: The findings revealed three key conclusions may be drawn from the findings: (1) In the sphere of NPOs, robust and conventional statistical methods-based data analysis procedures are dominantly common at all times; (2) Healthcare and public affairs are two crucial sectors that involve data analytics to support decision-making and problem-solving; (3) Artificial Intelligence (AI) based data analytics is a recently emerging trending, especially in the healthcare-related sector; however, it is still at an immature stage, and more efforts are needed to nourish its development.Conclusion: The research findings can leverage future research and add value to the existing literature on the subject of data analytics