Huaiqihuang may protect from proteinuria by resisting MPC5 podocyte damage via targeting p-ERK/CHOP pathway

The purpose of this study was to investigate the potential effects of Huaiqihuang (HQH) granule, a Chinese herbal medicine, in treating proteinuria and to reveal its possible mechanism. MPC5 podocytes were cultured in vitro at 37°C and induced with tunicamycin (TM). The TM-induced cells were treated with HQH at different concentrations. The cell proliferation was detected using the MTT assay. The optimal effective dose of HQH for MPC5 cells was determined by the MTT assay and LDH assay respectively. The influences of HQH on the proteinuria-related protein expression and the signaling pathway associated protein expression were also detected using quantitative reverse transcription PCR and Western blotting analysis. The results showed that the MPC5 cell model was successfully constructed in vitro. The HQH application could improve the harmful effects induced by TM on the MPC5 cells, including promoted cell proliferation and suppressed cell apoptosis. Furthermore, the protein expression, including podocin, nephrin, and synaptopodin was down-regulated by the TM treatment in the MPC5 cells. On contrary, the expression of these proteins was up-regulated after the HQH application. Also, the effect of TM on integrin α3 and integrin β1 expressions was also reversed by the HQH treatment. Moreover, the HQH application decreased the expression of p-ERK and DNA-damage-inducible transcript 3 (DDIT3 or CHOP) in the MPC5 cells, which was opposite to the effect observed in the cells treated with TM. Taken together, our study suggest that HQH application may protect podocytes from TM damage by suppressing the p-ERK/CHOP signaling pathway

Effect of Inflammation on the Process of Stroke Rehabilitation and Poststroke Depression

A considerable body of evidence has shown that inflammation plays an important role in the process of stroke rehabilitation and development of poststroke depression (PSD). However, the specific molecular and cellular mechanisms involved remain unclear. In this review, we summarize how neuroinflammation affects stroke rehabilitation and PSD. We mainly focus on the immune/inflammatory response, involving astrocytes, microglia, monocyte-derived macrophages, cytokines (tumor necrosis factor alpha, interleukin 1), and microRNAs (microRNA-124, microRNA 133b). This review provides new insights into the effect of inflammation on the process of stroke rehabilitation and PSD and potentially offer new therapeutic targets of stroke and PSD

Effect of transanal drainage tube on prevention of anastomotic leakage after anterior rectal cancer surgery taking indwelling time into consideration: a systematic review and meta-analysis

BackgroundPlacement of an indwelling transanal drainage tube (TDT) to prevent anastomotic leakage (AL) after anterior rectal cancer surgery has become a routine choice for surgeons in the recent years. However, the specific indwelling time of the TDT has not been explored. We performed this meta-analysis and considered the indwelling time a critical factor in re-analyzing the effectiveness of TDT placement in prevention of AL after anterior rectal cancer surgery.MethodsRandomized controlled trials (RCTs) and cohort studies which evaluated the effectiveness of TDT in prevention of AL after rectal cancer surgery and considered the indwelling time of TDT were identified using a predesigned search strategy in databases up to November 2022. This meta-analysis was performed to estimate the pooled AL rates (Overall and different AL grades) and reoperation rates at different TDT indwelling times and stoma statuses.ResultsThree RCTs and 15 cohort studies including 2381 cases with TDT and 2494 cases without TDT were considered eligible for inclusion. Our meta-analysis showed that the indwelling time of TDT for ≥5-days was associated with a significant reduction (TDT vs. Non-TDT) in overall AL (OR=0.46,95% CI 0.34-0.60, p<0.01), grade A+B AL (OR=0.64, 95% CI 0.42-0.97, p=0.03), grade C AL (OR=0.35, 95% CI 0.24-0.53, p<0.01), overall reoperation rate (OR=0.36, 95%CI 0.24-0.53, p<0.01) and that in patients without a prophylactic diverting stoma (DS) (OR=0.24, 95%CI 0.14-0.41, p<0.01). There were no statistically significant differences in any of the abovementioned indicators (p>0.05) when the indwelling time of TDT was less than 5 days.ConclusionExtending the postoperative indwelling time of TDT to 5 days may reduce the overall AL and the need for reoperation in patients without a prophylactic DS.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023407451, identifier CRD42023407451

A coupled 3D isogeometric and discrete element approach for modelling interactions between structures and granular matters

A three-dimensional (3D) isogeometric/discrete-element coupling method is presented for modelling contact/impact between structures and particles. This method takes advantages of the geometry smoothness and exactness of isogeometric analysis (IGA) for continuous solid media and the effectiveness and flexibility of the discrete element method (DEM) for particulate matters. The coupling procedure for handling interactions between IGA elements and discrete elements (DEs) includes global search, local search and interaction calculation. In the global search, the CGRID method is modified to detect potential contact pairs between IGA elements and DEs based on their bounding box representations. The strong convex hull property of a NURBS control mesh plays an important part in the bounding box representation of IGA elements. In the local search, the proposed approach treats each spherical DE centroid as a slave node and the contact surface of each IGA element as the master surface. The projection of a DE centroid onto an IGA element contact surface is solved by modifying the simplex method and Brent iterations. The contact force between an IGA element and a DE is determined from their penetration by using a (nonlinear) penalty function based method. The whole coupled system is solved by the explicit time integration within a updated Lagrangian scheme. Finally, three impact examples, including the impact of two symmetric bars, a tube onto a footing strip, and an assembly of granular particles to a tailor rolled blank, are simulated in elastic regime to assess the accuracy and applicability of the proposed method

An Isogeometric Bézier Finite Element Method for Vibration Optimization of Functionally Graded Plate with Local Refinement

An effective free vibration optimization procedure in combination with the isogeometric approach (IGA), particle swarm optimization (PSO) and an integrated global and local parameterization is presented. The natural frequency of functionally graded (FG) plates is calculated by the IGA based on the Bézier extraction of non-uniform rational B-splines (NURBS) with the cubic NURBS basis function. The material composition is assumed to vary only in the thickness direction, and the volumetric fraction is described by the NURBS basis function in light of the superior properties of NURBS curves. The volume fractions of the control points are then optimized by the PSO. In most of the previous work, the control points for the volume fraction are usually equally spaced, which is incapable of identifying the optimal location of the graded zones in most cases. To overcome this bottleneck, a novel local refinement strategy is proposed. The reliability and effectiveness of the proposed approach are demonstrated through several numerical examples. It is interesting to observe that the optimal results are sandwich or laminate plates, and few parameters are involved in the integrated global and local parameterization

An Isogeometric Bézier Finite Element Method for Vibration Optimization of Functionally Graded Plate with Local Refinement

An effective free vibration optimization procedure in combination with the isogeometric approach (IGA), particle swarm optimization (PSO) and an integrated global and local parameterization is presented. The natural frequency of functionally graded (FG) plates is calculated by the IGA based on the Bézier extraction of non-uniform rational B-splines (NURBS) with the cubic NURBS basis function. The material composition is assumed to vary only in the thickness direction, and the volumetric fraction is described by the NURBS basis function in light of the superior properties of NURBS curves. The volume fractions of the control points are then optimized by the PSO. In most of the previous work, the control points for the volume fraction are usually equally spaced, which is incapable of identifying the optimal location of the graded zones in most cases. To overcome this bottleneck, a novel local refinement strategy is proposed. The reliability and effectiveness of the proposed approach are demonstrated through several numerical examples. It is interesting to observe that the optimal results are sandwich or laminate plates, and few parameters are involved in the integrated global and local parameterization

Variational Formulations and Isogeometric Analysis of Timoshenko–Ehrenfest Microbeam Using a Reformulated Strain Gradient Elasticity Theory

This paper presents a novel non-classical Timoshenko–Ehrenfest beam model based on a reformulated strain gradient elasticity theory. The strain gradient effect, couple stress effect, and velocity gradient effect for vibration are included in the new model by only one material length scale parameter for each. The variational formulation and Hamilton’s principle are applied to derive the governing equations and boundary conditions. Both an analytical solution and an isogeometric analysis approach are proposed for static bending and free vibration of the microbeam. A non-uniform rational B-splines (NURBS) isogeometric analysis with high-order continuity can effectively fulfill the higher derivatives of the displacement variables in the reformulated gradient beam model. Convergence studies and comparisons to the corresponding analytical solutions verify the model’s performance and accuracy. Finally, different boundary conditions, material length scale parameters, and beam thicknesses are investigated in order to certify the applicability of the proposed approach

Size-Dependent Buckling Analysis of Microbeams by an Analytical Solution and Isogeometric Analysis

This paper proposes an analytical solution and isogeometric analysis numerical approach for buckling analysis of size-dependent beams based on a reformulated strain gradient elasticity theory (RSGET). The superiority of this method is that it has only one material parameter for couple stress and another material parameter for strain gradient effects. Using the RSGET and the principle of minimum potential energy, both non-classical Euler–Bernoulli and Timoshenko beam buckling models are developed. Moreover, the obtained governing equations are solved by an exact solution and isogeometric analysis approach, which conforms to the requirements of higher continuity in gradient elasticity theory. Numerical results are compared with exact solutions to reveal the accuracy of the current isogeometric analysis approach. The influences of length–scale parameter, length-to-thickness ratio, beam thickness and boundary conditions are investigated. Moreover, the difference between the buckling responses obtained by the Timoshenko and Euler–Bernoulli theories shows that the Euler–Bernoulli theory is suitable for slender beams