Detection and comparison of tumor cell-associated microbiota from different compartments of colorectal cancer

IntroductionIntratumoral microbes play an important role in the development of colorectal cancer (CRC). However, studying intratumoral microbes in CRC faces technical challenges, as tumor microbe communities are often contaminated by fecal microbes due to the structure of the gut folds and villi. The present study aimed to develop a new method for isolating tumor cell-associated microbiota and comparing microbial populations from different compartments.Materials and methodsThe distribution of intestinal bacteria was detected using immunohistochemistry combined with 5R-16s rRNA gene sequencing to explore the effects of the sampling site and number of washes on the detection of microbiota. The 5R-16s rRNA gene sequencing was performed using 44 samples from 11 patients with CRC, including CRC tumor tissues (TT), normal tissues adjacent to CRC (NT), tumor cells (TC), and normal cells (NC). TC and NC were obtained from the TT and NT using an enzymatic digestion method. The microbiota and their potential functions in the four groups were analyzed and compared to determine the differential microbiota related to CRC.ResultsBacteria were mainly distributed in the feces covering intestinal tissues and in the epithelial cells and macrophages within the tissues. Different sampling sites and number of washes led to detection of different microbiota distributions. Although the cleaning method could be controlled, sampling sites varied and led to different microbiota distributions. The phyla of Firmicutes and Bacteroidetes were highly abundant in the conventionally used tissue samples, whereas Proteobacteria was the most abundant phyla in the cell samples isolated with the new method (i.e., after cell enzymatic hydrolysis). Detection of CRC cell-associated microbiota using a cell enzymatic digestion method showed that some bacteria, such as Fusobacterium, Eikenella, Shewanella, and Listeria, were more abundant in TT than NT, whereas the abundance of Akkermansia was lower in TT than NT. The tumor/normal ratios of some bacteria, such as Gemella, Escherichia, Shigella, and Blautia, were different between the cell and tissue samples.ConclusionThe cell enzymatic digestion method reduced fecal bacterial contamination, enabling low biomass intratumoral microbiota to be detected and allowing prediction of bacterial distributions

Three-Dimensional Surface Displacement of the Eastern Beijing Plain, China, Using Ascending and Descending Sentinel-1A/B Images and Leveling Data

Surface displacement is an common environmental geological phenomenon in the Beijing Plain. Research on surface displacement in the Beijing Plain has mainly focused on vertical surface displacement, whereas the horizontal displacement has scarcely been studied. To investigate the 3-D surface displacement in the Beijing Plain, we construct a leveling-constrained multidirectional PS-InSAR 3-D surface displacement estimation method to obtain the 3-D surface displacement information. The results show that the surface displacement in the study area during 2016–2018 was mainly vertical displacement with two main northern and southern subsidence centers; the vertical displacement ranged from −150 mm/year (down) to 5 mm/year (up), and the east–west horizontal displacement ranged from 20 mm/year (east) to 22 mm/year (west). Validation results show that the 3-D surface displacement estimation results agree well with leveling data and GPS data, indicating the reliability of the 3-D surface displacement datasets. The 3-D surface displacement results show that horizontal displacement is obvious in the areas with a large vertical displacement in the eastern Beijing Plain. Additionally, the horizontal displacement is directed toward the center of vertical displacement. The compressive strain is observed close to the centers of vertical displacement, whereas tensile strain occurs far from the centers of vertical displacement. The main cause of the 3-D surface displacement in the study area is the long-term groundwater overexploitation, especially deep groundwater exploitation. The spatial and temporal extents of displacement do not exactly match the locations of the groundwater sinks in different aquifers; instead, geological structures and stratigraphic/lithological conditions may have a combined effect. Moreover, the spatial and temporal distributions of surface displacement are closely related to ground fissure activity, and both influence each other

Correction: Min, S., et al. Recent Ground Subsidence in the North China Plain, China, Revealed by Sentinel-1A Datasets. Remote Sensing 2020, 12, 3579

The authors wish to make the following corrections to this paper [...

Resistance Evaluation of Dominant Varieties against Southern Rice Black-Streaked Dwarf Virus in Southern China

Southern rice black-streaked dwarf virus (SRBSDV), a Fijivirus in the Reoviridae family, is transmitted by the white-backed planthopper (Sogatella furcifera, WBPH), a long-distance migratory insect, and presents a serious threat to rice production in Asia. It was first discovered in China’s Guangdong Province in 2001 and has been endemic in the south of China and north of Vietnam for two decades, with serious outbreaks in 2009, 2010, and 2017. In this study, we evaluated the resistance of 10 dominant rice varieties from southern China, where the virus overwinters and accumulates as a source of early spring reinfection, against this virus by artificial inoculation. The results showed that in all tested varieties there was no immune resistance, but there were differences in the infection rate, with incidence rates from 21% to 90.7%, and in symptom severity, with plant weight loss from 66.71% to 91.20% and height loss from 34.1% to 65.06%. Additionally, and valuably, the virus titer and the insect vector virus acquisition potency from diseased plants were significantly different among the varieties: an over sixfold difference was determined between resistant and susceptible varieties, and there was a positive correlation between virus accumulation and insect vector virus acquisition. The results can provide a basis for the selection of rice varieties in southern China to reduce the damage of SRBSDV in this area and to minimize the reinfection source and epidemics of the virus in other rice-growing areas

Hsa_circRNA_102051 regulates colorectal cancer proliferation and metastasis by mediating Notch pathway

Abstract Background The purpose of this study was to investigate the role of hsa_circRNA_102051 in colorectal cancer (CRC) and its effect on the stemness of tumor cells. Methods CircRNA microarray was under analysis to screen differentially expressed novel circRNAs in the pathology of CRC. Quantitative real-time PCR was used to detect the relative RNA expression in CRC cells and samples. The effects of hsa_circRNA_102051 on biological functions in CRC cells were accessed both in vitro and in vivo. FISH, RIP and luciferase reporter assay were conducted to confirm the regulatory correlations between hsa_circRNA_102051 and miR-203a, as well as miR-203a and BPTF. Xenograft models were applied to further verify the impacts and fluctuations of hsa_circRNA_102051/miR-203a/BPTF. Moreover, the mechanism how hsa_circRNA_102051 affected the Notch signals was also elucidated. Results Hsa_circRNA_102051 was up-regulated in CRC tissues and cell lines, capable to promote the growth and invasion of CRC. In addition, hsa_circRNA_102051 could enhance stemness of CRC cells. BPTF was identified as downstream factors of hsa_circRNA_102051, and miR-203a was determined directly targeting both hsa_circRNA_102051 and BPTF as an intermediate regulator. Hsa_circRNA_102051 in CRC could block miR-203a expression, and subsequently activated BPTF. Hsa_circRNA_102051/miR-203a/BPTF axis modulated stemness of CRC cells by affecting Notch pathway. Conclusions Our findings provided new clues that hsa_circRNA_102051 might be a potential predictive or prognostic factor in CRC, which induced the fluctuation of downstream miR-203a/BPTF, and subsequently influenced tumor growth, activities and stemness. Thereinto, the Notch signals were also involved. Hence, the hsa_circRNA_102051/miR-203a/BPTF axis could be further explored as a therapeutic target for anti-metastatic therapy in CRC patients