Assessment of integrated index of environmental units of the transport system

Transport-technological complex environmental impact, drivers and participants in the transport process exhaust gases, products of wear of tires, road surface, brake pads and discs and other harmful substances.. Proposed air pollution on roads and highways to estimate the complex index of atmospheric pollution transport and technological complex (APITTC)

Assessment of integrated index of environmental units of the transport system

Transport-technological complex environmental impact, drivers and participants in the transport process exhaust gases, products of wear of tires, road surface, brake pads and discs and other harmful substances.. Proposed air pollution on roads and highways to estimate the complex index of atmospheric pollution transport and technological complex (APITTC)

Optimization of technology for production of products of deep drawing sheet steels with the use of lubricants in universal software system ANSYS workbench

The techniques and laboratory tests, simulating the technological process of drawing sheet steels with water soluble lubricant PB-18 with a fractal structure, with the addition of nanopowders of copper and bronze. Given optimization of the stretching process of sheet steels using universal software system of finite-element analysis ANSYS Workbenc

Optimization of technology for production of products of deep drawing sheet steels with the use of lubricants in universal software system ANSYS workbench

The techniques and laboratory tests, simulating the technological process of drawing sheet steels with water soluble lubricant PB-18 with a fractal structure, with the addition of nanopowders of copper and bronze. Given optimization of the stretching process of sheet steels using universal software system of finite-element analysis ANSYS Workbenc

Unexpected Room Temperature Ferromagnetism of a Ball-Milled Graphene Oxide—Melamine Mixture

Nitrogen-doped carbon nanomaterial (NDCNM) was synthesized by grinding a mixture of graphene oxide and melamine in a planetary mill with both balls and milling chamber of zirconium dioxide. In the electron spin resonance spectrum of NDCNM, a broad signal with g = 2.08 was observed in addition to a narrow signal at g = 2.0034. In the study using a vibrating-sample magnetometer, the synthesized material is presumably a ferromagnet with a coercive force of 100 Oe. The specific magnetization at 10,000 Oe is approximately 0.020 and 0.055 emu/g at room temperature and liquid nitrogen temperature, respectively

On the State of Graphene Oxide Nanosheet in a Polyurethane Matrix

Thermally stable films were obtained from a water-based polyurethane (PU) dispersion with small (0.1–1.5 wt.%) additions of graphene oxide (GO). The films were studied through elemental analysis, X-ray photoelectron spectroscopy, differential thermogravimetry, and Raman spectroscopy. It was found that the introduction of GO into a PU matrix was accompanied by a partial reduction in graphene oxide nanosheet and an increase in the concentration of defects in GO structure. It has been also established that the [C/N]at ratio in the near-surface layer of PU/GO composite films grows with an increase in the content of graphene oxide in the composite films

Point Amino Acid Substitutions in The Ca\u3csup\u3e2+\u3c/sup\u3e-binding Sites of Recoverin: III. A Mutant with The Fourth Reconstructed Ca\u3csup\u3e2+\u3c/sup\u3e-binding Site

Unlike wild type recoverin with only two (the second and the third) functioning Ca+2-binding sites out of four potential ones, the +EF4 mutant contains a third active Ca+2-binding site. This site was reconstructed from the fourth potential Ca+2-binding domain by the introduction of several amino acid substitutions in it by site-directed mutagenesis. The effect of these mutations in the fourth potential Ca+2-binding site of myristoylated recoverin on the structural features and conformational stability of the protein was studied by fluorimetry and circular dichroism. The apoform of the resulting mutant (free of Ca2+ ions) was shown to have a higher calcium capacity, significantly lower thermal stability, and noticeably different secondary and tertiary structures as compared with the apoform of wild-type recoverin

A Facile Synthesis of Noble-Metal-Free Catalyst Based on Nitrogen Doped Graphene Oxide for Oxygen Reduction Reaction

A simple method for the mechanochemical synthesis of an effective metal-free electrocatalyst for the oxygen reduction reaction was demonstrated. A nitrogen-doped carbon material was obtained by grinding a mixture of graphene oxide and melamine in a planetary ball mill. The resulting material was characterized by XPS, EPR, and Raman and IR spectroscopy. The nitrogen concentration on the N-bmGO surface was 5.5 at.%. The nitrogen-enriched graphene material (NbmGO has half-wave potential of −0.175/−0.09 V and was shown to possess high activity as an electrocatalyst for oxygen reduction reaction. The electrocatalytic activity of NbmGO can be associated with a high concentration of active sites for the adsorption of oxygen molecules on its surface. The high current retention (93% for 12 h) after continuous polarization demonstrates the excellent long-term stability of NbmGO

Effects of Mutations in The Calcium-binding Sites of Recoverin on Its Calcium Affinity: Evidence for Successive Filling of The Calcium Binding Sites

A molecule of the photoreceptor Ca2+-binding protein recoverin contains four potential EF-hand Ca2+-binding sites, of which only two, the second and the third, are capable of binding calcium ions. We have studied the effects of substitutions in the second, third and fourth EF-hand sites of recoverin on its Ca2+-binding properties and some other characteristics, using intrinsic fluorescence, circular dichroism spectroscopy and differential scanning microcalorimetry. The interaction of the two operating binding sites of wild-type recoverin with calcium increases the protein\u27s thermal stability, but makes the environment around the tryptophan residues more flexible. The amino acid substitution in the EF-hand 3 (E121Q) totally abolishes the high calcium affinity of recoverin, while the mutation in the EF-hand 2 (E85Q) causes only a moderate decrease in calcium binding. Based on this evidence, we suggest that the binding of calcium ions to recoverin is a sequential process with the EF-hand 3 being filled first. Estimation of Ca2+-binding constants according to the sequential binding scheme gave the values 3.7 × 106 and 3.1 × 105 M–1 for third and second EF-hands, respectively. The substitutions in the EF-hand 2 or 3 (or in both the sites simultaneously) do not disturb significantly either tertiary or secondary structure of the apo-protein. Amino acid substitutions, which have been designed to restore the calcium affinity of the EF-hand 4 (G160D, K161E, K162N, D165G and K166Q), increase the calcium capacity and affinity of recoverin but also perturb the protein structure and decrease the thermostability of its apo-form