Modelling the geographical spread of HIV among MSM in Guangdong, China: a metapopulation model considering the impact of pre-exposure prophylaxis

Men who have sex with men (MSM) make up the majority of new human immunodeficiency virus (HIV) diagnoses among young people in China. Understanding HIV transmission dynamics among the MSM population is, therefore, crucial for the control and prevention of HIV infections, especially for some newly reported genotypes of HIV. This study presents a metapopulation model considering the impact of pre-exposure prophylaxis (PrEP) to investigate the geographical spread of a hypothetically new genotype of HIV among MSM in Guangdong, China. We use multiple data sources to construct this model to characterize the behavioural dynamics underlying the spread of HIV within and between 21 prefecture-level cities (i.e. Guangzhou, Shenzhen, Foshan, etc.) in Guangdong province: the online social network via a gay social networking app, the offline human mobility network via the Baidu mobility website, and self-reported sexual behaviours among MSM. Results show that PrEP initiation exponentially delays the occurrence of the virus for the rest of the cities transmitted from the initial outbreak city; hubs on the movement network, such as Guangzhou, Shenzhen, and Foshan are at a higher risk of 'earliest' exposure to the new HIV genotype; most cities acquire the virus directly from the initial outbreak city while others acquire the virus from cities that are not initial outbreak locations and have relatively high betweenness centralities, such as Guangzhou, Shenzhen and Shantou. This study provides insights in predicting the geographical spread of a new genotype of HIV among an MSM population from different regions and assessing the importance of prefecture-level cities in the control and prevention of HIV in Guangdong province. This article is part of the theme issue 'Data science approach to infectious disease surveillance'

hElp3 Directly Modulates the Expression of HSP70 Gene in HeLa Cells via HAT Activity

Human Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulation of the human elongation protein 3 (hELP3) on the expression of HSP70 through antisense strategy. Transfecting antisense plasmid p1107 into HeLa cells highly suppressed hELP3 expression, and substantially reduced expression of HSP70 mRNA and protein. Furthermore, chromatin immunoprecipitation assay (ChIP Assay) revealed that hElp3 participates in the transcription elongation of HSPA1A in HeLa cells. Finally, complementation and ChIP Assay in yeast showed that hElp3 can not only complement the growth and slow activation of HSP70 (SSA3) gene transcription, but also directly regulates the transcription of SSA3. On the contrary, these functions are lost when the HAT domain is deleted from hElp3. These data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function. These findings now provide novel insights and evidence of the functions of hELP3 in human cells

Emergence of Fatal PRRSV Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique Hallmark

Porcine reproductive and respiratory syndrome (PRRS) is a severe viral disease in pigs, causing great economic losses worldwide each year. The causative agent of the disease, PRRS virus (PRRSV), is a member of the family Arteriviridae. Here we report our investigation of the unparalleled large-scale outbreaks of an originally unknown, but so-called “high fever” disease in China in 2006 with the essence of PRRS, which spread to more than 10 provinces (autonomous cities or regions) and affected over 2,000,000 pigs with about 400,000 fatal cases. Different from the typical PRRS, numerous adult sows were also infected by the “high fever” disease. This atypical PRRS pandemic was initially identified as a hog cholera-like disease manifesting neurological symptoms (e.g., shivering), high fever (40–42°C), erythematous blanching rash, etc. Autopsies combined with immunological analyses clearly showed that multiple organs were infected by highly pathogenic PRRSVs with severe pathological changes observed. Whole-genome analysis of the isolated viruses revealed that these PRRSV isolates are grouped into Type II and are highly homologous to HB-1, a Chinese strain of PRRSV (96.5% nucleotide identity). More importantly, we observed a unique molecular hallmark in these viral isolates, namely a discontinuous deletion of 30 amino acids in nonstructural protein 2 (NSP2). Taken together, this is the first comprehensive report documenting the 2006 epidemic of atypical PRRS outbreak in China and identifying the 30 amino-acid deletion in NSP2, a novel determining factor for virulence which may be implicated in the high pathogenicity of PRRSV, and will stimulate further study by using the infectious cDNA clone technique

Fabricate the Auto-aquaculture Structure with Android Monitoring System

Based on the Android monitoring system, the automated fish feeder has been developed. The system provides a convenient and reliable solution for fish farmers. This system includes a fish feeder that distributes food at predetermined intervals. The Android application allows farmers to monitor and control the feeding process remotely. The application displays the current feeding schedule. At the same time, the users can adjust the frequency and amount of food dispensed. The alarm function can send the notification information to the farmer’s mobile phone if the feeder experiences any issues or requires maintenance. By automating the feeding process and providing real-time monitoring, the system can help farmers optimize fish growth and health while reducing the time and effort required for artificial feeding

Decentralizing Private Blockchain-IoT Network with OLSR

With data transparency and immutability, the blockchain can provide trustless and decentralized services for Internet of Things (IoT) applications. However, most blockchain-IoT networks, especially those with a private blockchain, are built on top of an infrastructure-based wireless network (i.e., using Wi-Fi access points or cellular base stations). Hence, they are still under the risk of Single-Point-of-Failure (SPoF) on the network layer, hindering the decentralization merit, for example, when the access points or base stations get failures. This paper presents an Optimized Link State Routing (OLSR) protocol-based solution for that issue in a private blockchain-IoT application. By decentralizing the underlying network with OLSR, the private blockchain network can avoid SPoF and automatically recover after a failure. Single blockchain connections can be extended to multiple ad hoc hops. Services over blockchain become flexible to fit various IoT scenarios. We show the effectiveness of our solution by constructing a private Ethereum blockchain network running on IoT devices (i.e., Raspberry Pi model 4) with environmental data sensing (i.e., Particular Matter (PM)). The IoT devices use OLSR to form an ad hoc network. The environment data are collected and propagated in transactions to a pre-loaded smart contract periodically. We then evaluate the IoT blockchain network’s recovery time when facing a link error. The evaluation results show that OLSR can automatically recover after the failure. We also evaluate the transaction-oriented latency and block-oriented latency, which indicates the blocks have a high transmission quality, while transactions are transferred individually

Robust Estimation Fusion in Wireless Senor Networks with Outliers and Correlated Noises

This paper addresses the problem of estimation fusion in a distributed wireless sensor network (WSN) under the following conditions: (i) sensor noises are contaminated by outliers or gross errors; (ii) process noise and sensor noises are correlated; (iii) cross-correlation among local estimates is unknown. First, to attack the correlation and outliers, a correlated robust Kalman filtering (coR 2 KF) scheme with weighted matrices on innovation sequences is introduced as local estimator. It is shown that the proposed coR 2 KF takes both conventional Kalman filter and robust Kalman filter as a special case. Then, a novel version of our internal ellipsoid approximation fusion (IEAF) is used in the fusion center to handle the unknown cross-correlation of local estimates. The explicit solution to both fusion estimate and corresponding covariance is given. Finally, to demonstrate robustness of the proposed coR 2 KF and the effectiveness of IEAF strategy, a simulation example of tracking a target moving on noisy circular trajectories is included

Two-Dimensional Electromagnetohydrodynamic (EMHD) Flows of Fractional Viscoelastic Fluids with Electrokinetic Effects

The present study provides analytical and numerical solutions for an electromagnetohydrodynamic (EMHD) flow using a Caputo time-fractional Maxwell model. The flow is a typical rectangular channel flow. When the scale of the cross-stream is much smaller than the streamwise and spanwise scales, the model is approximated as a two-dimensional slit parallel plate flow. Moreover, the influence of the electric double layer (EDL) at the solid–liquid interface is also considered. The electro-osmotic force generated by the interaction between the electric field and the EDL will induce a flow (i.e., electro-osmotic flow). Due to the application of the electric field at the streamwise and the vertical magnetic field, the flow is driven by Lorentz force along the spanwise direction. Simultaneously, under the action of the magnetic field, the electro-osmotic flow induces a reverse Lorentz force, which inhibits the electro-osmotic flow. The result shows that resonance behavior can be found in both directions in which the flow is generated. However, compared with the classical Maxwell fluid, the slip velocity and resonance behavior of fractional Maxwell fluid are suppressed. In the spanwise direction, increasing the strength of magnetic field first promotes the slip velocity and resonance behavior, and then suppresses them, while in the streamwise direction, both the electro-osmotic flow and resonance behavior are suppressed with the magnetic field

Recharging Schedule for Mitigating Data Loss in Wireless Rechargeable Sensor Network

Wireless Power Transfer (WPT) technology is considered as a promising approach to make Wireless Rechargeable Sensor Network (WRSN) work perpetually. In WRSN, a vehicle exists, termed a mobile charger, which can move close to sensor nodes and charge them wirelessly. Due to the mobile charger’s limited traveling distance and speed, not every node that needs to be charged may be serviced in time. Thus, in such scenario, how to make a route plan for the mobile charger to determine which nodes should be charged first is a critical issue related to the network’s Quality of Service (QoS). In this paper, we propose a mobile charger’s scheduling algorithm to mitigate the data loss of network by considering the node’s criticality in connectivity and energy. First, we introduce a novel metric named criticality index to measure node’s connectivity contribution, which is computed as a summation of node’s neighbor dissimilarity. Furthermore, to reflect the node’s charging demand, an indicator called energy criticality is adopted to weight the criticality index, which is a normalized ratio of the node’s consumed energy to its total energy. Then, we formulate an optimization problem with the objective of maximizing total weighted criticality indexes of nodes to construct a charging tour, subject to the mobile charger’s traveling distance constraint. Due to the NP-hardness of the problem, a heuristic algorithm is proposed to solve it. The heuristic algorithm includes three steps, which is spanning tree growing, tour construction and tour improvement. Finally, we compare the proposed algorithm to the state-of-art scheduling algorithms. The obtained results demonstrate that the proposed algorithm is a promising one