Additional file 1: of Establishment and molecular characterization of a human ovarian clear cell carcinoma cell line (FDOV1)

Table S1. The primer of Sanger sequence for specific genes mutation in FDOV1. (DOCX 15 kb

Predicted CitXET active sites.

<p>Predicted CitXET active sites.</p

Kaplan-Meier survival curves of venous thromboembolism (VTE) in ovarian CCC.

<p>(a). Overall survival analyzed for cases with or without VTE. (b). Progression-free survival analyzed for cases with or without VTE. (c) Overall survival analysis based on timing of VTE. (d) Progression-free survival analysis based on the timing of the VTE.</p

Clinicopathological features of ovarian CCC patients with or without VTE.

<p>*Chi-square test and parametric student <i>t</i> test.</p><p>Abbreviations: CCC, clear cell carcinoma; SD, standard deviation; VTE, venous thromboembolism; CA 125, cancer antigen 125.</p><p>Clinicopathological features of ovarian CCC patients with or without VTE.</p

Features of venous thromboembolism (VTE) in ovarian clear cell carcinoma.

<p>Abbreviations: VTE, venous thromboembolism; DVT, deep vein thrombosis; PE, pulmonary embolism.</p><p>Features of venous thromboembolism (VTE) in ovarian clear cell carcinoma.</p

Predicted CitXET secondary structures.

<p>Blue line, α-helix; Red line, extended chain; Green line, β-sheet; Purple line, random curl.</p

Figures of <i>Huangguogan</i> seedlings in 20 days after seed germination.

<p>H-E, etiolated seedlings. H-M, multicoloured seedlings. H-G, green seedlings.</p

Variability in <i>CitXET</i> expression and XET activity in <i>Citrus</i> cultivar <i>Huangguogan</i> seedlings with differed degrees of etiolation

<div><p>Considering the known effects of xyloglucan endotransglycosylase (XET) on plant growth and development, we aimed to determine whether XETs help to regulate the growth and elongation of <i>Huangguogan</i> shoots and roots. We confirmed a possible role for XET during seedling etiolation. Our results revealed that the roots of etiolated seedlings (H-E) were longer than those of green seedlings (H-G). However, shoot length exhibited the opposite pattern. We also observed positive and negative effects on the xyloglucan-degrading activity of XET in the root sub-apical region and shoots of etiolated <i>Huangguogan</i> seedling, respectively. There was a significant down-regulation in <i>CitXET</i> expression in the etiolated shoots at 15 days after seed germination. On the contrary, it was significantly increased in the root sub-apical region of etiolated and multicolored seedlings at 15 days after seed germination. The XET coding sequence (i.e., <i>CitXET</i>) was cloned from <i>Huangguogan</i> seedlings using gene-specific primers. The encoded amino acid sequence was predicted by using bioinformatics-based methods. The 990-bp <i>CitXET</i> gene was highly homologous to other <i>XET</i> genes. The CitXET protein was predicted to contain 319 amino acids, with a molecular mass of 37.45 kDa and an isoelectric point of 9.05. The predicted molecular formula was C₁₇₂₄H₂₅₄₈N₄₄₈O₄₆₆S₁₄, and the resulting protein included only one transmembrane structure. The CitXET secondary structure consisted of four main structures (i.e., 21% α-helix, 30.72% extended strand, 9.09% β-turn, and 39.18% random coil). Analyses involving the NCBI Conserved Domains Database (NCBI-CDD), InterPro, and ScanProsite revealed that CitXET was a member of the glycosyl hydrolase family 16 (GH16), and included the DEIDFEFLG motif. Our results indicate that the differed degrees of etiolation influenced the <i>CitXET</i> expression pattern and XET activity in <i>Huangguogan</i> seedlings. The differential changes in XET activity and <i>CitXET</i> expression levels in <i>Huangguogan</i> seedlings may influence the regulation of root and shoot development, and may be important for seedling etiolation.</p></div

Features of the CitXET molecular structure.

<p><b>a</b>: CitXET tertiary structures, N- to C-terminals are color-coded blue to red; <b>b</b>: Model-Template Alignment of CitXET and PttXET16A; <b>c</b>: QMEAN analysis.</p

Pulmonary emboli in both pulmonary arteries, as demonstrated by autopsy in a patient who died of pulmonary embolism.

<p>Please refer to the main text for the details of the case. Fig. 1a macroscopic thrombus (arrows); Fig. 1b a 20-cm long thrombus; Fig. 1c microscopic thrombus (arrows), hematoxylin and eosin stain, magnification X100.</p