Role of color doppler in the diagnosis of intra uterine growth restriction (IUGR)

Background: The purpose of our study was to evaluate the diagnostic efficacy of the pulsatility index (PI) and resistive index (RI) in uterine artery, umbilical artery and middle cerebral artery in the diagnosis of IUGR and prediction of adverse perinatal outcome.Methods: A total of 100 clinically suspected IUGR cases were enrolled in the study. A detailed history and examination was done, color doppler carried out serially every three weeks starting from 30 weeks till delivery, subsequently confirmation of fetal growth restriction (FGR) was done by assessing the newborn parameters for growth restriction.Results: Doppler measurement for uterine artery showed higher efficacy as compared to umbilical artery and middle cerebral artery findings. The uterine artery RI was found to be 84.6% sensitive and 82.9% specific even at 30 weeks. Uterine artery PI too showed a good diagnostic efficacy with an accuracy of 79%, a sensitivity of 76.9%, a specificity of 82.9%.Both PI and RI for uterine artery showed a relatively higher specificity.Conclusion: Here we concluded that once IUGR is suspected, Doppler velocimetry may be useful as a part of evaluation and uterine artery analysis identifies a subgroup with an increased risk for developing IUGR.

Overexpression, purification and localization of apoptosis related protein from Plasmodium falciparum

A growing body of evidence has ascertained that apoptosis is not only restricted to metazoans but also exists in unicellular parasites. In Plasmodium falciparum, the presence of a putative gene having sequence homology with apoptosis related protein (PfARP) (Gene ID PFI0450c) has raised enormous interest to unravel the function of this unique protein in cell death of malaria parasite. To characterize this protein, the PfARP gene has been amplified from the P. falciparum transcriptome by RT-PCR and the amplified gene has been successfully cloned, over-expressed and purified to homogeneity. The purified PfARP exhibits minimum subunit MW of ∼24 kDa as evident from SDS–PAGE. CD analysis reveals that the α and β content of the recombinant PfARP are 61% and 15%, respectively. Semiquantitative RT-PCR analysis indicates the expression of PfARP at both metabolically less active ring and highly active trophozoite stages of malaria parasite. Immunofluorescence microscopy further supports that PfARP expresses stage specifically with the highest expression at trophozite stage and very little in the schizont stage. PfARP is a cytosolic protein as evident from immunofluorescence microscopy. The role of this protein in P. falciparum cell death and stage progression is not yet known. The identification, purification and characterization would certainly be a step to initiate work on this protein to evaluate its role in P. falciparum growth, multiplication and stage progression