11 research outputs found
Cognitive Sub-Nyquist Hardware Prototype of a Collocated MIMO Radar
We present the design and hardware implementation of a radar prototype that
demonstrates the principle of a sub-Nyquist collocated multiple-input
multiple-output (MIMO) radar. The setup allows sampling in both spatial and
spectral domains at rates much lower than dictated by the Nyquist sampling
theorem. Our prototype realizes an X-band MIMO radar that can be configured to
have a maximum of 8 transmit and 10 receive antenna elements. We use frequency
division multiplexing (FDM) to achieve the orthogonality of MIMO waveforms and
apply the Xampling framework for signal recovery. The prototype also implements
a cognitive transmission scheme where each transmit waveform is restricted to
those pre-determined subbands of the full signal bandwidth that the receiver
samples and processes. Real-time experiments show reasonable recovery
performance while operating as a 4x5 thinned random array wherein the combined
spatial and spectral sampling factor reduction is 87.5% of that of a filled
8x10 array.Comment: 5 pages, Compressed Sensing Theory and its Applications to Radar,
Sonar and Remote Sensing (CoSeRa) 201
Systematic identification of abundant A-to-I editing sites in the human transcriptome
RNA editing by members of the double-stranded RNA-specific ADAR family leads
to site-specific conversion of adenosine to inosine (A-to-I) in precursor
messenger RNAs. Editing by ADARs is believed to occur in all metazoa, and is
essential for mammalian development. Currently, only a limited number of human
ADAR substrates are known, while indirect evidence suggests a substantial
fraction of all pre-mRNAs being affected. Here we describe a computational
search for ADAR editing sites in the human transcriptome, using millions of
available expressed sequences. 12,723 A-to-I editing sites were mapped in 1,637
different genes, with an estimated accuracy of 95%, raising the number of known
editing sites by two orders of magnitude. We experimentally validated our
method by verifying the occurrence of editing in 26 novel substrates. A-to-I
editing in humans primarily occurs in non-coding regions of the RNA, typically
in Alu repeats. Analysis of the large set of editing sites indicates the role
of editing in controlling dsRNA stability.Comment: Pre-print version. See http://dx.doi.org/10.1038/nbt996 for a reprin
Adaptive proteome diversification by nonsynonymous A-to-I RNA editing in coleoid cephalopods
© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Shoshan, Y., Liscovitch-Brauer, N., Rosenthal, J. J. C., & Eisenberg, E. Adaptive proteome diversification by nonsynonymous A-to-I RNA editing in coleoid cephalopods. Molecular Biology and Evolution, 38(9), (2021): 3775–3788, https://doi.org/10.1093/molbev/msab154.RNA editing by the ADAR enzymes converts selected adenosines into inosines, biological mimics for guanosines. By doing so, it alters protein-coding sequences, resulting in novel protein products that diversify the proteome beyond its genomic blueprint. Recoding is exceptionally abundant in the neural tissues of coleoid cephalopods (octopuses, squids, and cuttlefishes), with an over-representation of nonsynonymous edits suggesting positive selection. However, the extent to which proteome diversification by recoding provides an adaptive advantage is not known. It was recently suggested that the role of evolutionarily conserved edits is to compensate for harmful genomic substitutions, and that there is no added value in having an editable codon as compared with a restoration of the preferred genomic allele. Here, we show that this hypothesis fails to explain the evolutionary dynamics of recoding sites in coleoids. Instead, our results indicate that a large fraction of the shared, strongly recoded, sites in coleoids have been selected for proteome diversification, meaning that the fitness of an editable A is higher than an uneditable A or a genomically encoded G.This research was supported by a grants from the United States–Israel Binational Science Foundation (BSF), Jerusalem, Israel (BSF2017262 to J.J.C.R. and E.E.), the Israel Science Foundation (3371/20 to E.E.) and the National Science Foundation (IOS 1827509 and 1557748 to J.J.C.R)