The organization of the virus-tested planting material production for the grape varieties of the local and foreign selection in Kazakhstan

The intensification of viniculture involves the organization of the virus-tested planting material production, establishment of the basic parent plantings, certification of the virus-tested planting material with the control of genetic stability of the grape plants propagated in tissue culture. The modified nutritional medium was developed for microclonal propagation of vine in vitro with the content of the free amino acids: glycine and glutamine, increasing propagation factor up to 15% and the number of nodes on microplant up to 27%

Micropropagation of some grape varieties in Kazakhstan

The article shows the improvement of the process of initiation into in vitro the culture and the clonal micropropagation of grape varieties. The optimal culture media for the initiation and cloning of grapes in vitro have been selected. During initiation on Murashige and Skoog, agar medium with ½ or ¾ concentration of macroand micronutrients and hormones (0.5 mg/l BAP and 0.1 mg/l IBA) is optimal. For micropropagation is suitable MS medium modified by some elements of mineral nutrition: 825 mg/l NH4NO3, 166 mg/l CaCl2, 15 mg/l ferrum chelate; best hormonal composition depends on variety: a) 0.5-1 mg/l BAP and 0.1-0.5 mg/l IBA; b) 0.5 mg/l 2-iP and 0.5 mg/l GA3