Engineering Safety Evaluation of the High Rock Slope of a Hydropower Project: A Case Study of 684 m-High Slope Related to Lianghekou Hydropower Project at Yalong River

The stability of the slope is a very important topic in the construction of hydropower projects, especially the slope engineering in the dam area, as its stability will directly affect the safety of engineering. Taking the inlet slope of the flood discharge structure of Lianghekou Hydropower Project as the research object, based on the analysis and exploration of the geological condition of the slope and the field monitoring data, GA-LSSVM is used to establish the non-linear mapping relationship, and the BP neural network is used to establish the mechanical parameters back analysis of the slope at different water impoundment stages. A numerical simulation model is also established to set up different reservoir impoundments to study the stability and sensibility of the slope and provide guidance for slope operation. This case study shows that there is a hysteresis in the response of slope deformation to reservoir impoundment. At the same time, the mechanical parameters of the slope will be weakened by the seepage. In the process of water level changes, the stability of the slope decreases due to the decrease in mechanical parameters. The study will be practically useful for engineering applications

m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma

Abstract Background Lung adenocarcinoma (LUAD) is a common type of lung cancer with a high risk of metastasis, but the exact molecular mechanisms of metastasis are not yet understood. Methods This study acquired single-cell transcriptomics profiling of 11 distal normal lung tissues, 11 primary LUAD tissues, and 4 metastatic LUAD tissues from the GSE131907 dataset. The lung multicellular ecosystems were characterized at a single-cell resolution, and the potential mechanisms underlying angiogenesis and metastasis of LUAD were explored. Results We constructed a global single-cell landscape of 93,610 cells from primary and metastatic LUAD and found that IGF2BP2 was specifically expressed both in a LUAD cell subpopulation (termed as LUAD_IGF2BP2), and an endothelial cell subpopulation (termed as En_IGF2BP2). The LUAD_IGF2BP2 subpopulation progressively formed and dominated the ecology of metastatic LUAD during metastatic evolution. IGF2BP2 was preferentially secreted by exosomes in the LUAD_IGF2BP2 subpopulation, which was absorbed by the En_IGF2BP2 subpopulation in the tumor microenvironment. Subsequently, IGF2BP2 improved the RNA stability of FLT4 through m6A modification, thereby activating the PI3K-Akt signaling pathway, and eventually promoting angiogenesis and metastasis. Analysis of clinical data showed that IGF2BP2 was linked with poor overall survival and relapse-free survival for LUAD patients. Conclusions Overall, these findings provide a novel insight into the multicellular ecosystems of primary and metastatic LUAD, and demonstrate that a specific LUAD_IGF2BP2 subpopulation is a key orchestrator promoting angiogenesis and metastasis, with implications for the gene regulatory mechanisms of LUAD metastatic evolution, representing themselves as potential antiangiogenic targets