Electrochemistry of myoglobin on graphene–SnO2 nanocomposite modified electrode and its electrocatalysis

AbstractIn this paper direct electrochemistry and electrocatalysis of myoglobin (Mb) immobilized on a graphene (GR)–SnO2 nanocomposite modified carbon ionic liquid electrode was reported. GR–SnO2 nanocomposite was synthesized by a simple solution method and further characterized by TEM and SEM, which exhibited large surface area beneficial for Mb immobilization. Spectroscopic results indicated Mb retained its native structure without denaturation after mixed with nanocomposite. Electrochemical investigation showed that a pair of well-defined redox peaks appeared on cyclic voltammogram, indicating that direct electron transfer of Mb with the underlying electrode was realized. The results could be attributed to the presence of GR–SnO2 nanocomposite that could enhance the electron transfer between the protein and the electrode. The Mb modified electrode exhibited good stability and catalytic activity to the electroreduction of NaNO2 in the concentration range from 0.2 to 350.0μmolL−1 with wider dynamic range and lower detection limit. Therefore the fabricated electrode has the potential application in the third-generation electrochemical biosensor

Broadening the media of communication of agricultural information and its role in agricultural development

In IDL-1120

Study on Stress Relief of Hard Roof Based on Presplitting and Deep Hole Blasting

For the problem that the hard roof causes wider end-mining coal pillar, and the roadway is greatly affected by mining, this paper took Shanxi Luning Coal Mine as the engineering background; based on the stress distribution characteristics of the coal pillar, the calculation method of the limit end-mining coal pillar size was given; considering the formation conditions and transmission forms of the advanced abutment stress, a method combining presplitting and deep hole blasting was proposed to weaken the advanced abutment stress. The numerical simulation was used to analyze the stress distribution of coal pillars, which was verified by on-site industrial tests. The results showed that the presplitting can achieve the blocking of stress. The closer it is to the peak of the abutment stress, the better the blocking effect. Deep hole blasting can weaken the source of the advanced abutment stress and reduce the peak of abutment stress. With the combination of the two blasting methods, the end-mining coal pillar size of Luning Coal Mine can be reduced to 60 m. The method combining presplitting and deep hole blasting can effectively reduce the end-mining coal pillar size and reduce the impact of mining on the deformation of the dip roadway

Analysis of Failure Characteristics and Strength Criterion of Coal-Rock Combined Body with Different Height Ratios

In underground mining and roadway support engineering of coal mine, the coal and rock layers bear loads together; therefore, the deformation and mechanical characteristics of the coal-rock combined bodies are not the same as those of the pure coal or rock bodies. In this paper, conventional triaxial compression tests of coal-rock combined bodies with different height ratios were conducted. And the stress and deformation characteristics of coal-rock combined body were studied and the experimental results were analyzed with different strength criteria. The results show that the peak stress, elastic modulus, and strength reduction coefficient of coal-rock combined body are negatively correlated with the ratio of coal to coal-rock combination height and positively correlated with the confining pressure; the coal-rock combination shows obvious ductility under 10 MPa confining pressure. Under the conventional triaxial condition, the shear failure was the main cause of the lateral deformation of the coal body in the coal-rock combination, which was much larger than that of the rock body. The circle deformation value, volume strain value, and the deformation rate in the postpeak stage of coal-rock combination are much higher than those in the prepeak stage. Mohr–Coulomb and general Hoek–Brown strength criterion fit the experimental results well

Study on the Porosity of Saturated Fragmentized Coals during Creep Process and Constitutive Relation

Pore abundance and deformation characteristics of saturated fragmentized coals during creep process are of significant meaning to the study on ground sediment in the mined-out area. The law of porosity variation of saturated fragmentized coals during creep process and its creep constitutive model were studied by using the self-developed multiphase coupling creep test device. And, results have indicated that the porosity logarithm of fragmentized coal during creep process shows a linear negative correlation with the time ln(n−a) = −ct + lnb, and the porosity decrease is evidently divided into three phases. In addition, when the stress level is relatively low, the porosity decreases slowly; when the stress level rises up, the porosity decreases quickly; when the stress level remains stable finally, the porosity is smaller. Under the equal stress, as the grain size of fragmentized coals decreases, the porosity tends to decrease, and as the grain size of fragmentized coal tends to be stable, the porosity tends to increase; the creep constitutive equation of fragmentized coals with different grain sizes was established by using the Kelvin–Voigt model, and the correlation analysis shows that the Kelvin–Voigt creep model of fragmentized coals is reasonable

Particle Flow Code Simulation of the Characteristics of Crack Evolution in Rock-Like Materials with Bent Cracks

The distribution and propagation of rock cracks have a significant impact on geotechnical engineering. Taking rock-like materials with bent cracks as the research object, the particle flow code in two dimensions numerical simulation method was used to study the impact of the bend number on rock-like materials strength and crack evolution. According to the results, when the bend number was 1, 3, and 7, the strength of the specimens gradually increased; the elasticity modulus did not change significantly with the crack bend number. Uniaxial compression generated tensile cracks in all the specimens with bent cracks, but in terms of failure mode, the specimens with 0 bend tended to suffer penetrating failure along the fracture strike, while the specimens with 1, 3, and 7 bend tended to suffer penetrating failure along the diagonal direction. Both the fractal dimension and bend number were positively correlated with strain; with the gradual increase of the stress percentage, the damage variable of the specimens gradually increased at a growing rate. The research results provide a reference for predicting the stability of the underground engineering surrounding rocks containing bent cracks

Controlling the Deformation of a Small Coal Pillar Retaining Roadway by Non-Penetrating Directional Pre-Splitting Blasting with a Deep Hole: A Case Study in Wangzhuang Coal Mine

In longwall mining of coal mines, the large deformation of small pillar retaining roadways creates difficulties for the safe and efficient retreating of the mining panel. Based on the engineering background of a small coal pillar retaining roadway in Wangzhuang coal mine, pressure relief technology for non-penetrating directional pre-splitting blasting with a deep hole ahead was proposed. The influence of the non-penetrating fracture length on the pre-splitting effect was studied by numerical simulation. The results showed that the vertical stress in the coal pillar center, the small pillar retaining roadway deformation, and the energy accumulation on the pillar decreased with an increase in the non-penetrating fracture length. The vertical stress at the working face end increased with an increase in the non-penetrating fracture length. The field application and monitoring results indicated that non-penetrating directional pre-splitting blasting could effectively control the deformation of small pillar retaining roadways. The roof-to-floor and rib-to-rib maximum convergences of the 6208 tail entry were reduced by 53.66% and 52.62%, respectively, compared to the results with no blasting. The roadway section met the demands of mining panel high-efficiency retreating, thereby demonstrating the rationality of the technical and numerical simulation results. The research results shed light on the improvement of small coal pillar retaining roadway maintenance theory and technology

Simulation Research on the Load Transfer Mechanism of Anchoring System in Soft and Hard Composite Rock Strata under Tensile Loading Conditions

Soft and hard composite rock strata are frequently encountered in transportation, geotechnical, and underground engineering. However, most of the current support is designed for homogeneous rock masses, which ignores the different anchoring effect in soft and hard composite rock strata. A numerical study is presented in this paper on the pull-out behavior of fully grouted rock bolts in soft and hard composite rock strata. The nonlinear bond-slip relationship of bolt-grout interface that is anchored in soft rock and hard rock is obtained from laboratory test, respectively. Then, the nonlinear bond-slip relationship is put into the numerical model. The numerical result shows a close match with the experiment tests and the proposed model. Lithological sequence, layer thickness ratio, and layer numbers are taken into consideration in numerical simulation models. Under the same layer number, the shallower-soft and deeper-hard composite rock strata (SHCRS) have a higher bearing capacity and deformation resistance than the shallower-hard and deeper-soft composite rock strata (HSCRS). As the soft-to-hard thickness ratio in SHCRS increases, the initial stiffness of the load-displacement curve and peak load decreases continuously. The load-displacement curve shows the same initial stiffness for different hard to soft thickness ratios in HSCRS. As the hard to soft thickness ratio increases, the load peak and the displacement at the peak load increase. Therefore, the closer the hard rock is to the loading end, the greater the initial stiffness of the load-displacement curve is. The greater the hard rock thickness, the larger the peak load. Under the same anchor length, the peak load and the displacement at the peak load decrease with the increase of layer numbers, but the reduction magnitude decreases. This paper leads to a better understanding of the load transfer mechanism for the anchoring system in soft and hard composite strata and provides a reference for scientific support design and evaluation method