The Effects of Flooding on Shirakawa Delta Morphology

Floods can significantly alter the morphology of a delta, from inputting increased volumes of sediments to modifying the reach of incoming waves, currents, and other gravity-driven forces occurring within the near shore. Kumamoto experienced flooding in July 2012. This flood event altered the equilibrium profile of the intertidal flat area of the Shirakawa River. This research looks at these modifications and attempts to explain their long-term implications on the overall delta morphology. Data collected over a 30-year period was analyzed and profiles were generated to better analyze and assess the trend in delta morphological changes. Numerical predictions on delta morphology in the presence of gravitydriven sediment transport were applied to investigate the morphological changes related to the flood event. The slope of equilibrium profile is lower and steeper offshore, helping to explain the models created before and after the flood occurred

Flood Risk Management: An Illustrative Approach

Widespread flooding with significant damage in many countries, such as the Philippines in 2013, highlights the ongoing need for effective flood risk management (FRM). This hinges on comprehensive access to and dissemination of information about the elements and the people at risk. Simulations, real-time graphs, and maps illustrate the spatial distribution of flood risks, spatial allocation and dissemination of flood effects, if flood risk reduction measures are not implemented, as well as the benefits to be derived from the effective implementation and maintenance of flood risk management measures not realized. Using precipitation, river water, and tide levels, a real-time monitoring site was set up for the Shirakawa River, Kumamoto, Japan. The data gathered from the July 2012 flood event is used as a demonstrator, illustrating a flood event as well as how to utilize the information provided on this site to determine the future time and possibility of flooding. Additionally, an electronically generated flood hazard map making process is being developed for distribution across Japan. These illustrative approaches can be utilized in cities and communities around the globe

Simultaneous CXCL12 and ESR1 CpG island hypermethylation correlates with poor prognosis in sporadic breast cancer

<p>Abstract</p> <p>Background</p> <p>CXCL12 is a chemokine that is constitutively expressed in many organs and tissues. <it>CXCL12 </it>promoter hypermethylation has been detected in primary breast tumours and contributes to their metastatic potential. It has been shown that the oestrogen receptor α (<it>ESR1</it>) gene can also be silenced by DNA methylation. In this study, we used methylation-specific PCR (MSP) to analyse the methylation status in two regions of the <it>CXCL12 </it>promoter and <it>ESR1 </it>in tumour cell lines and in primary breast tumour samples, and correlated our results with clinicopathological data.</p> <p>Methods</p> <p>First, we analysed <it>CXCL12 </it>expression in breast tumour cell lines by RT-PCR. We also used 5-aza-2'-deoxycytidine (5-aza-CdR) treatment and DNA bisulphite sequencing to study the promoter methylation for a specific region of <it>CXCL12 </it>in breast tumour cell lines. We evaluated <it>CXCL12 </it>and <it>ESR1 </it>methylation in primary tumour samples by methylation-specific PCR (MSP). Finally, promoter hypermethylation of these genes was analysed using Fisher's exact test and correlated with clinicopathological data using the Chi square test, Kaplan-Meier survival analysis and Cox regression analysis.</p> <p>Results</p> <p><it>CXCL12 </it>promoter hypermethylation in the first region (island 2) and second region (island 4) was correlated with lack of expression of the gene in tumour cell lines. In the primary tumours, island 2 was hypermethylated in 14.5% of the samples and island 4 was hypermethylated in 54% of the samples. The <it>ESR1 </it>promoter was hypermethylated in 41% of breast tumour samples. In addition, the levels of ERα protein expression diminished with increased frequency of <it>ESR1 </it>methylation (p < 0.0001). This study also demonstrated that <it>CXCL12 </it>island 4 and <it>ESR1 </it>methylation occur simultaneously at a high frequency (p = 0.0220).</p> <p>Conclusions</p> <p>This is the first study showing a simultaneous involvement of epigenetic regulation for both <it>CXCL12 </it>and <it>ESR1 </it>genes in Brazilian women. The methylation status of both genes was significantly correlated with histologically advanced disease, the presence of metastases and death. Therefore, the methylation pattern of these genes could be used as a molecular marker for the prediction of breast cancer outcome.</p