<ORIGINAL REPORT>CYTOCHEMICAL LOCALIZATION OF GLUCOSE 6-PHOSPHATASE IN CILIATED CELLS OF RAT TRACHEAL EPITHELIUM

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。The tracheobronchial epithelium is a ciliated epithelium. Its ciliary movement plays a role of cleaning the respiratory passage. For the ciliary movement, ciliated epithelial cells have probably large energy requirements and glucose from the blood is an important source of the energy. Therefore, it is of interest whether, or how, glucose 6-phosphatase (G-6-Pase), an important enzyme in glucose metabolism, is localized in the cells. Female Sprague-Dawley rats, about 100g, were used. They had free access to food and water prior to experiments. About 0.5mm thick cross-sectioned tracheae adjacent to the bifurcation of the trachea were fixed in 2% glutaraldehyde buffered with 0.1M cacodylate (pH7) for 30 minutes at 4℃ and washed in 0.1M cacodylate (pH 7) for 1 hour at 4℃. The tissues were cut into 30μ sections by a freezing microtome and incubated in Wachstein and Meisel's medium for the demonstration of G-6-Pase for 45 minutes at room temperature. The sections were postfixed in buffered 1% osmium tetroxide for 1 hour at 4℃, dehydrated in graded series of alcohol and embedded in Epon. Thin sections were cut with glass knives on a LKB ultratome, stained with uranyl acetate and lead citrate and examined in a JEM-7 A electron microscope. Control experiments were carried out as described previously. The reaction product was present in the endoplasmic reticulum and nuclear envelope of ciliated cells, together with goblet cells. Golgi complex, mitochondria, plasma membrane and other organellae of these cells showed no deposits of final product. This pattern of localization is entirely equal to that of the enzyme in hepatocytes and other cells, although new findings were expected because ciliated cells probably consume a large amount of glucose as a energy source of the ciliary movement. G-6-Pase has an important role in carbohydrate metabolism, In the liver and kidney, gluconeogenesis and glycogenolysis are important pathways, and the role of G-6-Pase is to release glucose into the blood from these organs. However, the physiologic function this enzyme is unknown in a variety of other organs. Arion et al. have recently suggested that the physiologically functional form of this enzyme is specific for glucose 6-phosphate and hydrolysis of glucose 6-phosphate is the sole function of the enzyme in vivo. On the other hand, tracheal ciliated cells and goblet cells probably have large glucose 6-phosphate requirement as the energy source for the ciliary movement and material for biosynthesis of secretory glycoprotein. Thus, these cells possibly have a potent ability to produce glucose 6-phosphate from glucose in the blood. One might, therefore, postulate that the role of G-6-Pase is to regulate the level of concentration of glucose 6-phosphate in these cells, hydrolyzing excess glucose 6-phosphate produced by these cells

ULTRASTRUCTURAL CYTOCHEMISTRY OF THE ALDEHYDE-SENSITIVE ENZYMES : LOCALIZATION OF GLUCOSE 6-PHOSPHATASE ACTIVITY IN MOUSE HEPATOCYTES

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。Ultrastructural localization of glucose 6-phosphatase activity was investigated in mouse hepatocytes. The reaction product for the enzyme activity was present in the endoplasmic reticulum and nuclear envelope, as in rat hepatocytes. The physiological significance of the presence of the enzyme in the endoplasmic reticulum is discussed

<Original Report>DECLINE IN GLUCOSE-6-PHOSPHATASE ACTIVITY DURING PROLONGED POSTFIXATION WASHING

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました

エチオニン シエンカタンソオヨビエタノールニヨルシボウカンニオケルシボウサンゴウセイコウソノソシキカガクテキケンキュウ

京都大学0048新制・論文博士医学博士乙第1960号論医博第484号新制||医||166(附属図書館)2897UT51-47-B477(主査)教授 高松 英雄, 教授 岡本 耕造, 教授 沼 正作学位規則第5条第2項該当Kyoto UniversityDA

<ORIGINAL REPORT>FIXATION OF LUNG FOR ELECTRON MICROSCOPY : FIXATION OF FRESH FROZEN SECTIONS WITH OSMIUM TETROXIDE

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。A fixation technique for the ultrastructural observation of the lung is presented. This technique is useful in very small animals in which the intratracheal infusion and vascular perfusion of fixatives are difficult to perform