Estimating total lysine requirement for optimised egg production of broiler breeder hens during the early-laying period

The production performance of broiler breeder hens in response to different levels of total lysine during the early laying period was investigated. A total of 126 Ross 308 parent stock hens were offered one of seven dietary treatments formulating elevated contents of total lysine ranging from 0.55% to 0.79% (0.04 scale" 133 g of feed) from 23 to 29 weeks of age. Each treatment had six replicates with three birds per pen. Body weight was recorded triweekly and eggs were collected and weighted at 9:00 am daily. One hen from each pen was euthanized to collect blood samples and visceral organs were harvested and weighed. Egg production, egg weight and egg mass were lower (

Multi-Carbohydrase Addition Into a Corn-Soybean Meal Diet Containing Wheat and Wheat By Products to Improve Growth Performance and Nutrient Digestibility of Broiler Chickens

Feed enzymes are used in poultry diets to enhance nutrient availability and thereby improve growth performances of the birds. The objective of the present study was to investigate the effect of dietary Multi-Carbohydrase (MC) supplementation on growth performance, blood metabolites, visceral organ weights, gut morphology, and nutrient digestibility in broiler chickens. A total of 168 one-day-old broiler chicks (47.5 ± 0.20 g) were randomly allocated into one of four dietary treatments in a 2 × 2 factorial arrangement with 6 replications per treatment. Dietary treatments were as follows: (1) positive control (PC; energy sufficient, 3,200 ME, kcal/kg); (2) negative control (NC; energy deficient, 3,100 ME, kcal/kg); (3) PC with MC (MC; SuperzymeCSTM; 0.05%); (4) NC with MC. Greater ADG (P = 0.022) was observed with the birds fed MC for the entire period (1–35 d) compared to birds fed a diet without MC. Birds fed the NC diet supplemented with MC showed improved FCR (P = 0.037) compared to birds fed the NC diet during the starter period (1–21 d). MC supplementation increased dry matter (P = 0.029), crude protein (P = 0.015), and energy digestibility (P = 0.015) of the birds compared to those fed a diet without MC on day 21. Moreover, birds fed a diet with MC had increased (P = 0.037) dry matter digestibility on day 35 compared to its counterpart, regardless of dietary energy level. Therefore, our study indicated that MC improved growth performance along with nutrient digestibility in broiler chickens fed corn soybean-meal-based diets containing wheat and wheat by-products from hatch to 35 d of age, regardless of dietary energy level

Exogenous emulsifiers and multi-enzyme combination improves growth performance of the young broiler chickens fed low energy diets containing vegetable oil

Objective: The present study examined the effects of exogenous emulsifiers and multienzyme supplementation into a low energy density diet on growth performance, visceral organ parameters, blood metabolites, ileal morphology, and nutrient digestibility in broiler chickens from hatch to 21 days. Methods: One hundred and sixty-eight one-day-old Ross 308 broiler chickens were allocated in a completely randomized design to 24 pens and each pen was assigned to one of four dietary treatments to give six replications with seven chickens in a cage. Dietary treatments were: i) positive control with standard energy level (PC); ii) negative control with 100 kcal/kg lower energy of the standard level (NC); iii) NC diet supplemented 0.05% calcium stearoyl-2 lactylate as an emulsifier (NC+E); and iv) NC diet supplemented with both 0.05% calcium stearoyl-2 lactylate and 0.05% multi-enzyme (NC+E+M). Corn and soybean meal-based control diets containing vegetable oil were formulated to meet the Ross 308 nutrition specification. Chickens were fed ad-libitum with the treatment diets and sampling was conducted on day 21. Results: Our results revealed that emulsifier and multi-enzyme supplementation into NC diets improved (p0.05) visceral organ weight, blood metabolites, and intestinal morphology in broiler chickens fed NC diets. Conclusion: Supplementation of emulsifier and multi-enzyme in the NC diet would support improving growth performance in young broiler chickens with improved feed efficiency and increased nutrient digestibility thereby curtailing the negative impact of energy reduction in the diets

Differential Effects of Dietary Methionine Isomers on Broilers Challenged with Acute Heat Stress

In this study, we investigated the effect of methionine isomers (D- and L-methionine) on growth performance, blood metabolite levels, nutrient digestibility, intestinal morphology, and foot pad dermatitis in broilers challenged with acute heat stress. In total, 240 broilers were randomly allocated in a 2×2 factorial arrangement consisting of two dietary treatments (D- vs. L-methionine) and two thermal environmental conditions (thermo-neutral vs. acute heat stress). Methionine isomers were added to the diet as an ingredient according to the diet formulation. The broilers were exposed to acute heat stress at 33°C for 5 h on day 14. The average daily gain and feed conversion ratio of birds fed L-methionine were higher than those fed D-methionine (P<0.05) from the time of hatching till 21 days. Induced acute heat stress impaired (P<0.05) the daily gain and feed intake of the broilers on day 21. Furthermore, the blood urea nitrogen levels of birds subjected to acute heat stress on days 14 and 21 were higher (P<0.05) than those of their counterparts. Longer villi (P<0.05) were observed in broilers fed L-methionine-supplemented diet than in those fed D-methionine-supplemented diet on day 14, irrespective of thermal environmental conditions. Heat stress reduced (P<0.01) nutrient digestibility of the broilers on days 14 and 21. Higher incidence and severity of foot pad dermatitis were observed (P<0.05) in broilers fed diet containing D-methionine than in those fed L-methionine-supplemented diet. In conclusion, L-methionine-supplemented diet improved growth performance, overcame growth depression, and reduced the incidence of foot pad dermatitis when broilers were exposed to acute heat stress in the starter period

Differential effects of dietary methionine isomers on broilers challenged with acute heat stress

In this study, we investigated the effect of methionine isomers (D- and L-methionine) on growth performance, blood metabolite levels, nutrient digestibility, intestinal morphology, and foot pad dermatitis in broilers challenged with acute heat stress. In total, 240 broilers were randomly allocated in a 2×2 factorial arrangement consisting of two dietary treatments (D- vs. L-methionine) and two thermal environmental conditions (thermo-neutral vs. acute heat stress). Methionine isomers were added to the diet as an ingredient according to the diet formulation. The broilers were exposed to acute heat stress at 33℃ for 5 h on day 14. The average daily gain and feed conversion ratio of birds fed L-methionine were higher than those fed D-methionine (P＜0.05) from the time of hatching till 21 days. Induced acute heat stress impaired (P＜0.05) the daily gain and feed intake of the broilers on day 21. Furthermore, the blood urea nitrogen levels of birds subjected to acute heat stress on days 14 and 21 were higher (P＜0.05) than those of their counterparts. Longer villi (P＜0.05) were observed in broilers fed L-methionine-supplemented diet than in those fed D-methionine-supplemented diet on day 14, irrespective of thermal environmental conditions. Heat stress reduced (P ＜0.01) nutrient digestibility of the broilers on days 14 and 21. Higher incidence and severity of foot pad dermatitis were observed (P＜0.05) in broilers fed diet containing D-methionine than in those fed L-methionine-supplemented diet. In conclusion, L-methionine-supplemented diet improved growth performance, overcame growth depression, and reduced the incidence of foot pad dermatitis when broilers were exposed to acute heat stress in the starter period

Bioaccumulation and Toxicity Studies of Lead and Mercury in Laying Hens: Effects on Laying Performance, Blood Metabolites, Egg Quality and Organ Parameters

This study investigated bioaccumulation and toxicity derived from heavy metals in laying hens. The 160 52-week old laying hens were divided into 5 treatments with 8 replicates of 4 birds per pen. The treatments consisted of the control diet (without heavy metals), control diet with half the available dosage (AD, 5 ppm lead and 0.2 ppm mercury), AD (10 ppm lead and 0.4 ppm mercury), 2-fold AD (20 ppm lead and 0.8 ppm mercury), and 3-fold AD (30 ppm lead and 1.2 ppm mercury), and were provided to the laying hens for 8 weeks. Food and water were provided on an ad libitum basis at all times. Body weight and food intake were recorded once every two weeks, and eggs were collected and recorded daily. Two birds from each pen were euthanized to collect blood and organ samples on week 4 and 8. The 3-fold AD diet reduced food intake compared to that of the control and AD diets (P<0.05). Hens fed the half AD diet had darker yolk compared to those fed the control and AD diet on week 4 (P<0.05). Hens fed the 2- and 3-fold AD diets had increased relative liver weight, blood glutamic pyruvic transaminase and glutamic oxaloacetic transaminase levels (P<0.05), while F1 follicle weights decreased on week 4 and 8. No difference was found in egg production rate, egg quality, ovarian follicle, blood metabolites including protein, globulin, albumin, and urea nitrogen throughout the study (P>0.05). Heavy metal concentrations in the liver, eggs, and feathers were not detected at both week 4 and 8. Our results indicate that in-feed heavy metals for layer diets up to 30 ppm of lead and 1.2 ppm of mercury brought on hepatic dysfunction increasing blood metabolites that are associated with liver inflammation

Bioaccumulation and toxicity studies of lead and mercury in laying hens: Effects on laying performance, blood metabolites, egg quality and organ parameters

This study investigated bioaccumulation and toxicity derived from heavy metals in laying hens. The 160 52- week old laying hens were divided into 5 treatments with 8 replicates of 4 birds per pen. The treatments consisted of the control diet (without heavy metals), control diet with half the available dosage (AD, 5 ppm lead and 0.2 ppm mercury), AD (10 ppm lead and 0.4 ppm mercury), 2-fold AD (20 ppm lead and 0.8 ppm mercury), and 3-fold AD (30 ppm lead and 1.2 ppm mercury), and were provided to the laying hens for 8 weeks. Food and water were provided on an ad libitum basis at all times. Body weight and food intake were recorded once every two weeks, and eggs were collected and recorded daily. Two birds from each pen were euthanized to collect blood and organ samples on week 4 and 8. The 3-fold AD diet reduced food intake compared to that of the control and AD diets (P＜0.05). Hens fed the half AD diet had darker yolk compared to those fed the control and AD diet on week 4 (P＜0.05). Hens fed the 2- and 3-fold AD diets had increased relative liver weight, blood glutamic pyruvic transaminase and glutamic oxaloacetic transaminase levels (P＜0.05), while F1 follicle weights decreased on week 4 and 8. No difference was found in egg production rate, egg quality, ovarian follicle, blood metabolites including protein, globulin, albumin, and urea nitrogen throughout the study (P＞0.05). Heavy metal concentrations in the liver, eggs, and feathers were not detected at both week 4 and 8. Our results indicate that in-feed heavy metals for layer diets up to 30 ppm of lead and 1.2 ppm of mercury brought on hepatic dysfunction increasing blood metabolites that are associated with liver inflammation

Broilers fed a low protein diet supplemented with synthetic amino acids maintained growth performance and retained intestinal integrity while reducing nitrogen excretion when raised under poor sanitary conditions

The present study investigated the effects of supplementing a low protein (LP) diet supplemented with key essential amino acids (AA) to broilers on growth performance, intestinal tract function, blood metabolites, and nitrogen excretion when the animals were maintained under various sanitary conditions for 35 D after hatching. Three hundred eighty-four one-day-old male broilers (Ross 308) were randomly allotted to groups that received one of 6 dietary treatments in a 2 × 3 factorial arrangement (i.e., 2 environmental conditions and 3 dietary treatments) to give 8 replicates per treatment. Broilers were challenged with 2 environmental conditions (sanitary vs. poor sanitary). The dietary treatments were (1) high protein (HP) diet, (2) LP diet, and (3) LP diet with synthetic key essential AA (LPA): the LP diet was supplemented with synthetic AA up to the required levels for broilers. On day 14, birds consumed the LP diet impaired growth performance compared with those fed the HP diet, while the average daily weight gain-to-feed conversion ratio of birds fed the LPA diet improved to the level of birds fed the HP diet under poor sanitary conditions (