The changes of suicidal ideation status among young people in Hong Kong during COVID-19: A longitudinal survey

Background: Pandemics affect the physical and mental well-being of all potentially at-risk individuals. The secondary consequences of measures to prevent the outbreak (i.e., school closures) have extensive impact on young people globally. This longitudinal study examines changes of suicidal ideation status among adolescents during COVID-19. Method: A follow-up after nine-months of a school-based survey among 1,491 secondary students was conducted during COVID-19 in Hong Kong. Psychological well-being, psychological factors, family support, and COVID-19-related experiences were examined. Findings: The prevalence of suicidal ideation were 24% and 21% among the participants before and during COVID-19, respectively. In particular, 897 (65.0%) remained non-suicidal, 193 (14.0%) recovered from being suicidal, 148 (10.7%) newly reported being suicidal, and 143 (10.4%) remained suicidal. Respondents who remained suicidal were found to have significantly higher depression, anxiety, stress, loneliness, and social anxiety; fixed mindset; lower meaning of life and self-control; and lower parental support and supervision than the other groups. Participants with suicidal ideation reported more negative perceptions about the pandemic than non-suicidal participants. Logistic regression showed that participants with higher trait anxiety and higher stress from self-expectations in the baseline survey predicted higher likelihood of having suicidal ideation than the non-suicidal students at follow-up. Conclusion: Poor psychological well-being, lower level of family support, and negative impacts of the pandemic were consistently associated with students’ presence of suicidal ideation during the pandemic. Further intervention studies are needed to examine the effects of the mental health consequences of COVID-19 on youth mental health and to promote positive youth well-being

Leisure and problem gaming behaviors among children and adolescents during school closures caused by covid-19 in hong kong: Quantitative cross-sectional survey study

Background: School closures during the COVID-19 pandemic may have exacerbated students' loneliness, addictive gaming behaviors, and poor mental health. These mental health issues confronting young people are of public concern. Objective: This study aimed to examine the associations between loneliness and gaming addiction behaviors among young people in Hong Kong and to investigate how familial factors, psychological distress, and gender differences moderate these relationships. Methods: This cross-sectional study was conducted in June 2020 when schools reopened after 6 months of school closures. Participants included 2863 children and adolescents in primary (Grades 4 to 6) and secondary (Grades 7 and 8) schools (female participants: 1502/2863, 52.5%). Chi-square tests, one-way analyses of variance, and independent-samples t tests were performed to compare the differences of distribution in gaming addiction behaviors across gender, age, and other sociodemographic characteristics. Multinomial logistic regression analyses were conducted to identify factors that relate to excessive or pathological gaming behaviors separately, in comparison with leisure gaming.Results: A total of 83.0% (2377/2863) of the participants played video games during the COVID-19 pandemic. The prevalence of excessive and pathological game addiction behaviors was 20.9% (597/2863) and 5.3% (153/2863), respectively. More male students had gaming addiction symptoms than female students. The multinomial logistic regressions showed that feeling lonely was associated with more problematic gaming behaviors, and the association was stronger for older female students. Low socioeconomic status, less parental support and less supervision, and poor mental health were risk factors for gaming addiction behaviors, especially among primary school students. Conclusions: Loneliness was associated with gaming addiction behaviors; the findings from this study suggested that this association was similar across gender and age groups among young people. Familial support and supervision during school closures can protect young people from developing problematic gaming behaviors. Results of this study have implications for prevention and early intervention on behalf of policy makers and game developers

Risk assessment of metals from shallow groundwater in Lianhuashan District, China

The present study was conducted to investigate trace metal (Fe, Mn, Cd, Pb, Zn, Cu, Al, As, Se, Hg, Sr, Ba, Li, Co, B, Ni, Ag, Mo and Cr) concentrations of drinking water samples in Lianhuashan District, China. Furthermore, the study aimed to ascertain carcinogenic and non-carcinogenic health risks of metals by ingestion and dermal absorption pathways to the local residents. Metal concentrations were compared with permissible limits set by the Chinese Standards, USEPA and WHO. The average concentrations of the analyzed elements are in the order of Fe > Sr > Mn > Al > Ba > Mo > Zn > B > Li > Pb > Cr > Ni > Cu > Ag > Co > As > Se > Cd > Hg. The results indicate that the concentrations of Cd, Zn, Cu, Ni, Se, Hg, Ba and Cr were lower than their respective permissible limits, whereas the concentrations of Fe, Mn, Pb, Al, As and Mo at some sampling sites exceeded the permissible limits. The total hazard index (HQtotal) of the metals (Cd, Zn, Cu, Al, Se, Hg, Sr, Ba, B, Ni, Ag, Cr) calculated through ingestion and dermal absorption pathways for adults and children were found to be lower than unity, indicating that the metals would not pose any adverse effect and non-carcinogenic health risk to the habitants. The results of carcinogenic risk assessment indicate that the Excess Lifetime Cancer Risk (ingestion and dermal exposure pathways) of metals exposure was in accordance to the acceptable lifetime risks for carcinogens in drinking water (10−6–10−4). Both non-carcinogenic and carcinogenic risks were mainly attributed to the ingestion pathways

DNA-PK facilitates piggyBac transposition by promoting paired-end complex formation.

The involvement of host factors is critical to our understanding of underlying mechanisms of transposition and the applications of transposon-based technologies. Modified piggyBac (PB) is one of the most potent transposon systems in mammals. However, varying transposition efficiencies of PB among different cell lines have restricted its application. We discovered that the DNA-PK complex facilitates PB transposition by binding to PB transposase (PBase) and promoting paired-end complex formation. Mass spectrometry analysis and coimmunoprecipitation revealed physical interaction between PBase and the DNA-PK components Ku70, Ku80, and DNA-PKcs Overexpression or knockdown of DNA-PK components enhances or suppresses PB transposition in tissue culture cells, respectively. Furthermore, germ-line transposition efficiency of PB is significantly reduced in Ku80 heterozygous mutant mice, confirming the role of DNA-PK in facilitating PB transposition in vivo. Fused dimer PBase can efficiently promote transposition. FRET experiments with tagged dimer PBase molecules indicated that DNA-PK promotes the paired-end complex formation of the PB transposon. These data provide a mechanistic explanation for the role of DNA-PK in facilitating PB transposition and suggest a transposition-promoting manipulation by enhancing the interaction of the PB ends. Consistent with this, deletions shortening the distance between the two PB ends, such as PB vectors with closer ends (PB-CE vectors), have a profound effect on transposition efficiency. Taken together, our study indicates that in addition to regulating DNA repair fidelity during transposition, DNA-PK also affects transposition efficiency by promoting paired-end complex formation. The approach of CE vectors provides a simple practical solution for designing efficient transposon vectors

DNA-PK facilitates piggyBac transposition by promoting paired-end complex formation.

The involvement of host factors is critical to our understanding of underlying mechanisms of transposition and the applications of transposon-based technologies. Modified piggyBac (PB) is one of the most potent transposon systems in mammals. However, varying transposition efficiencies of PB among different cell lines have restricted its application. We discovered that the DNA-PK complex facilitates PB transposition by binding to PB transposase (PBase) and promoting paired-end complex formation. Mass spectrometry analysis and coimmunoprecipitation revealed physical interaction between PBase and the DNA-PK components Ku70, Ku80, and DNA-PKcs Overexpression or knockdown of DNA-PK components enhances or suppresses PB transposition in tissue culture cells, respectively. Furthermore, germ-line transposition efficiency of PB is significantly reduced in Ku80 heterozygous mutant mice, confirming the role of DNA-PK in facilitating PB transposition in vivo. Fused dimer PBase can efficiently promote transposition. FRET experiments with tagged dimer PBase molecules indicated that DNA-PK promotes the paired-end complex formation of the PB transposon. These data provide a mechanistic explanation for the role of DNA-PK in facilitating PB transposition and suggest a transposition-promoting manipulation by enhancing the interaction of the PB ends. Consistent with this, deletions shortening the distance between the two PB ends, such as PB vectors with closer ends (PB-CE vectors), have a profound effect on transposition efficiency. Taken together, our study indicates that in addition to regulating DNA repair fidelity during transposition, DNA-PK also affects transposition efficiency by promoting paired-end complex formation. The approach of CE vectors provides a simple practical solution for designing efficient transposon vectors