12 research outputs found

    High-density Integrated Linkage Map Based on SSR Markers in Soybean

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    A well-saturated molecular linkage map is a prerequisite for modern plant breeding. Several genetic maps have been developed for soybean with various types of molecular markers. Simple sequence repeats (SSRs) are single-locus markers with high allelic variation and are widely applicable to different genotypes. We have now mapped 1810 SSR or sequence-tagged site markers in one or more of three recombinant inbred populations of soybean (the US cultivar ‘Jack’ × the Japanese cultivar ‘Fukuyutaka’, the Chinese cultivar ‘Peking’ × the Japanese cultivar ‘Akita’, and the Japanese cultivar ‘Misuzudaizu’ × the Chinese breeding line ‘Moshidou Gong 503’) and have aligned these markers with the 20 consensus linkage groups (LGs). The total length of the integrated linkage map was 2442.9 cM, and the average number of molecular markers was 90.5 (range of 70–114) for the 20 LGs. We examined allelic diversity for 1238 of the SSR markers among 23 soybean cultivars or lines and a wild accession. The number of alleles per locus ranged from 2 to 7, with an average of 2.8. Our high-density linkage map should facilitate ongoing and future genomic research such as analysis of quantitative trait loci and positional cloning in addition to marker-assisted selection in soybean breeding

    Child Well-being in Japan: The high cost of economic success

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    This Innocenti Occasional Paper examines the counter-intuitive relationship between Japan’s continuing economic achievement and the good of its child population. Although child physical health and school attainment statistics have never looked better, changes in the structure of the family are having a worrying impact upon the collective psychological well-being of young Japanese. Adults and the elderly are harvesting the greater share of the benefits of economic success; the country’s children are missing out.child welfare; family structure; health statistics; school attendance;Japan;

    A comparison of methods for successful triggering of gene silencing in Coprinus cinereus

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    Post-transcriptional gene-silencing methods (PTGS), including RNAi, are becoming pervasive in functional genomics. To advance analysis of the recently sequenced Coprinus cinereus genome, a high throughput gene silencing method is essential. We have exploited the GFP reporter gene to evaluate and quantify efficacy of three different silencing strategies. Modular constructs that encompassed antisense, untranslatable sense, and RNAi-mediating hairpin sequences, were transformed into a GFP-expressing host strain. Transformants exhibiting strong downregulation and partial suppression of GFP were recovered with all three constructs. Analyses of protein and transcriptional nucleic acids revealed that the antosense and hairpin sequences yielded similar levels of GFP suppression, and were both more efficient than untranslatable sense sequence. Our antisense vectors will expedite functional characterisation of C. cinereus and the modular nature of the constructs should permit exploitation of directional cDNA libraries for high throughput screening
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