Deoxycholic Acid Could Induce Apoptosis and Trigger Gastric Carcinogenesis on Gastric Epithelial Cells by Quantitative Proteomic Analysis

Background. Pathologic duodenogastric reflux can induce or aggravate gastritis because of the presence of bile acids. Bile reflux has been generally considered to be associated with intestinal metaplasia and gastric cancer. However, the pathogenic mechanisms of the effects of bile acids on gastric mucosa are still unknown. Methods. To explore the mechanisms by which bile acids induce gastric mucosal lesions, we examined cell apoptosis in the gastric epithelial cell line GES-1 and investigated the changes in protein profiles of GES-1 cells in response to a bile acid deoxycholic acid using a proteomics approach. Changes in the profiles of the differently expressed proteins were analyzed using the DAVID and STRING programs. Results. We found apoptosis was significantly induced in GES-1 cells by deoxycholic acid. Using liquid chromatographic/tandem mass spectrometric (LC-MS/MS) methods, 134 upregulated proteins and 214 downregulated proteins were identified in the bile acid treated GES-1 cells. Bioinformatics analysis revealed the interactions and signaling networks of these differentially expressed proteins. Conclusion. These findings may improve the understanding of the molecular mechanisms underlying the pathogenicity of bile acids on gastric mucosa.Key laboratory for Helicobacter pylori infection and Upper Gastrointestinal Diseases, Beijing Key Laboratory [BZ0371]; Chinese National Natural Science Foundation of China [81270475]SCI(E)[email protected]

PROPERTIES OF GAS AND CHAR FROM MICROWAVE PYROLYSIS OF PINE SAWDUST

Pine sawdust pyrolysis was carried out respectively using microwave and conventional electrical heating at different temperatures in order to understand the properties of pyrolytic products from microwave pyrolysis of biomass. Less char material was obtained by microwave pyrolysis compared to conventional heating at the same temperature. While comparing the components of the pyrolytic gases, it was revealed that the microwave pyrolysis gas usually had higher H2 and CO contents and lower CH4 and CO2 contents than those obtained by conventional pyrolysis at the same temperature. The texture analysis results of the microwave pyrolysis chars showed that the chars would melt and the pores would shrink at high temperatures, and hence, the specific surface areas of the chars decreased with increasing temperature. Similarly, the reactivity of the char was remarkably reduced when the microwave pyrolysis temperature exceeded 600°C

Impacts of distributed thermal and electric contact resistance on performance and geometric optimization of thermoelectric generators

Thermal and electric contact resistance (TCR/ECR) critically impact performance and geometric optimization of thermoelectric generators (TEGs). However, conventional treatments usually ignored or simplified them as lumped variables, neglecting their actual distributions across the TEG system. In this study, we proposed a multi-physical model to characterize TEG performance with explicitly specifying TCRs/ECRs at different TEG interfaces (locations). The numerical results show that the lumped-variabletreatment led to maximal overestimations of 16.9 % and 24.5 % in the TEG output power and efficiency, respectively, compared to the results with distributed TCR in this article. Importantly, it also reveals that the TEG performance was susceptible to the TCR location—the interfaces on the cold side exerted more negative impacts than those on the hot side. Furthermore, reducing both TCR and ECR could improve TEG performance and reducing TCR is more effective. It is shown that an 80 % reduction in TCR increased the maximum TEG output power by 35.6 %, while the same reduction percentage in ECR only improved it by 8.8 %. As to geometric optimization, an optimal TE leg height equal to 0.6 mm was obtained for the maximum output power. This contrasts with previous studies without considering TCR and ECR, which always favoured shorter heights. As for copper electrodes, their optimal heights were in the range of 0.2–0.4 mm corresponding to the maximum efficiency, far smaller than those (0.7–1.2 mm) obtained when TCR/ECR were neglected. The latter even further resulted in a reduction in the maximum efficiency by more than 1 % compared to its true peak. In this study, all these numerical results clearly elucidate the important impacts of distributed TCR and ECR on TEG performance, and provide a comprehensive and balanced guideline for TEG design

High-dimensional frequency conversion in hot atomic system

One of the major difficulties in realizing a high-dimensional frequency converter for conventional optical vortex (COV) stems from the difference in ring diameter of COV modes with different topological charge numbers l. Here, we implement a high-dimensional frequency convertor for perfect optical vortex (POV) modes with invariant size through the four-wave mixing (FWM) process by utilizing Bessel-Gaussian beams instead of Laguerre-Gaussian beams. The measured conversion efficiency from 1530 nm to 795 nm is independent of l at least in subspace of {-6,...,6}, and the achieved conversion fidelities for two-dimensional (2D) superposed POV states exceed 97%. We further realize the frequency conversion of 3D, 5D and 7D superposition states with fidelities as high as 96.70%, 89.16% and 88.68%, respectively. The reported scheme is implemented in hot atomic vapor, it's also compatible with the cold atomic system and may find applications in high-capacity and long-distance quantum communication

The role of the transcription factor Rbpj in the development of dorsal root ganglia

<p>Abstract</p> <p>Background</p> <p>The dorsal root ganglion (DRG) is composed of well-characterized populations of sensory neurons and glia derived from a common pool of neural crest stem cells (NCCs), and is a good system to study the mechanisms of neurogenesis and gliogenesis. Notch signaling is known to play important roles in DRG development, but the full scope of Notch functions in mammalian DRG development remains poorly understood.</p> <p>Results</p> <p>In the present study, we used <it>Wnt1-Cre </it>to conditionally inactivate the transcription factor Rbpj, a critical integrator of activation signals from all Notch receptors, in NCCs and their derived cells. Deletion of <it>Rbpj </it>caused the up-regulation of <it>NeuroD1 </it>and precocious neurogenesis in DRG early development but led to an eventual deficit of sensory neurons at later stages, due to reduced cell proliferation and abnormal cell death. In addition, gliogenesis was delayed initially, but a near-complete loss of glia was observed finally in <it>Rbpj</it>-deficient DRG. Furthermore, we found P75 and Sox10, which are normally expressed exclusively in neuronal and glial progenitors of the DRG after the NCCs have completed their migration, were co-expressed in many cells of the DRG of <it>Rbpj </it>conditional knock-out mice.</p> <p>Conclusions</p> <p>Our data indicate that Rbpj-mediated canonical Notch signaling inhibits DRG neuronal differentiation, possibly by regulating <it>NeuroD1 </it>expression, and is required for DRG gliogenesis <it>in vivo</it>.</p