Influence of water temperature, habitat complexity and light on the predatory performance of the dark sleeperOdontobutis potamophila(Gunther, 1861)

Recently, the dark sleeper [Odontobutis potamophila(Gunther, 1861)] was selected as a potential aquaculture target. However, the feeding behavior of this species remains unclear. In this study, we investigated the influence of water temperature, habitat type, light intensity and photoperiod on the predatory performance of dark sleepers against juvenile demersal mrigal carp [Cirrhinus mrigala(Hamilton, 1822)] in the laboratory. Our results showed that both the prey consumption and the feeding rate of dark sleepers increased significantly with increases in water temperature (22-34 degrees C) and stabilized between 26-34 degrees C. Additionally, both the prey consumption and the feeding rate were higher in vegetated habitats than those in open areas at 22-30 degrees C, although no significant differences were detected. The prey consumption and the feeding rate of dark sleepers increased significantly with decreased light intensity (0-1350 lx) and shortened light duration (0-14 h), indicating that this fish prefers feeding in dark environments. These results will help elucidate the influence of environmental variables on this species' predatory performance and create suitable protocols for its culture

Single-cell RNA sequencing in cancer: Applications, advances, and emerging challenges

Cancer has become one of the greatest threats to human health, and new technologies are urgently needed to further clarify the mechanisms of cancer so that better detection and treatment strategies can be developed. At present, extensive genomic analysis and testing of clinical specimens shape the insights into carcinoma. Nevertheless, carcinoma of humans is a complex ecosystem of cells, including carcinoma cells and immunity-related and stroma-related subsets, with accurate characteristics obscured by extensive genome-related approaches. A growing body of research shows that sequencing of single-cell RNA (scRNA-seq) is emerging to be an effective way for dissecting human tumor tissue at single-cell resolution, presenting one prominent way for explaining carcinoma biology. This review summarizes the research progress of scRNA-seq in the field of tumors, focusing on the application of scRNA-seq in tumor circulating cells, tumor stem cells, tumor drug resistance, the tumor microenvironment, and so on, which provides a new perspective for tumor research