Atp6v1c1 may regulate filament actin arrangement in breast cancer cells.

Previous studies have shown that the rate of breast cancer metastasis correlates with the expression of vacuolar H(+)-ATPases (V-ATPases). However, how V-ATPase is involved in breast cancer metastasis remains unknown. Our previous study showed that Atp6v1c1-depleted osteoclasts did not form organized actin rings and that Atp6v1c1 co-localizes with F-actin. In this study, we found that the normal arrangement of filamentous actin is disrupted in Atp6v1c1-depleted 4T1 mouse breast cancer cells and in the ATP6V1C1-depleted human breast cancer cell lines MDA-MB-231 and MDA-MB-435s. We further found that Atp6v1c1 co-localizes with F-actin in 4T1 cells. The results of our study suggest that high expression of Atp6v1c1 affects the actin structure of cancer cells such that it facilitates breast cancer metastasis. The findings also indicate that Atp6v1c1 could be a novel target for breast cancer metastasis therapy

Regular F-actin arrangement was blocked in Atp6v1c1-depleted 4T1 cells.

<p>F-actin Oregon Green<b>®</b> 514 phalloidin staining of different 4T1 cells as indicated. Nuclei were visualized with DAPI. The cells shown are representative of the data (n = 3).</p

Atp6v1c1 co-localized with F-actin in 4T1 cells.

<p>Anti-Atp6v1c1 immunostaining and F-actin Oregon Green<b>®</b> 514 phalloidin staining of 4T1 cells. In the merged image, yellow staining showed that F-actin and Atp6v1c1 colocalized in the plasma and plasma membrane of 4T1 cells. The white arrows showed that most of the F-actin and Atp6v1c1 colocalization focused on the plasma membrane. The cells shown are representative of the data (n = 3).</p

Single-Site Active Iron-Based Bifunctional Oxygen Catalyst for a Compressible and Rechargeable Zinc–Air Battery

The exploitation of a high-efficient, low-cost, and stable non-noble-metal-based catalyst with oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) simultaneously, as air electrode material for a rechargeable zinc–air battery is significantly crucial. Meanwhile, the compressible flexibility of a battery is the prerequisite of wearable or/and portable electronics. Herein, we present a strategy <i>via</i> single-site dispersion of an Fe–N_<i>x</i> species on a two-dimensional (2D) highly graphitic porous nitrogen-doped carbon layer to implement superior catalytic activity toward ORR/OER (with a half-wave potential of 0.86 V for ORR and an overpotential of 390 mV at 10 mA·cm^–2 for OER) in an alkaline medium. Furthermore, an elastic polyacrylamide hydrogel based electrolyte with the capability to retain great elasticity even under a highly corrosive alkaline environment is utilized to develop a solid-state compressible and rechargeable zinc–air battery. The creatively developed battery has a low charge–discharge voltage gap (0.78 V at 5 mA·cm^–2) and large power density (118 mW·cm^–2). It could be compressed up to 54% strain and bent up to 90° without charge/discharge performance and output power degradation. Our results reveal that single-site dispersion of catalytic active sites on a porous support for a bifunctional oxygen catalyst as cathode integrating a specially designed elastic electrolyte is a feasible strategy for fabricating efficient compressible and rechargeable zinc–air batteries, which could enlighten the design and development of other functional electronic devices