A \u3cem\u3eLIN28B\u3c/em\u3e Tumor-Specific Transcript in Cancer

The diversity and complexity of the cancer transcriptome may contain transcripts unique to the tumor environment. Here, we report a LIN28B variant, LIN28B-TST, which is specifically expressed in hepatocellular carcinoma (HCC) and many other cancer types. Expression of LIN28B-TST is associated with significantly poor prognosis in HCC patients. LIN28B-TST initiates from a de novo alternative transcription initiation site that harbors a strong promoter regulated by NFYA but not c-Myc. Demethylation of the LIN28B-TST promoter might be a prerequisite for its transcription and transcriptional regulation. LIN28B-TST encodes a protein isoform with additional N-terminal amino acids and is critical for cancer cell proliferation and tumorigenesis. Our findings reveal a mechanism of LIN28B activation in cancer and the potential utility of LIN28B-TST for clinical purposes

Nonlinear analysis of RC shell structures using laminated element. II

An incremental variational formulation and curved-shell element were proposed in the companion paper. In this part, the material model and solution techniques are discussed in detail. The strain-hardening plastic approach is employed to model the compressive behavior of the concrete. A dual criterion is considered for yielding and crushing in terms of stresses and strains, which is completed with a tension cut-off representation. Both the crack interface effects and dowel action are accounted for using an average shear modulus, and a full bond is assumed at the steel-concrete interface. A total Lagrangian approach making use of the second Piola-Kirchhoff stress tensor is employed in this analysis. An incremental and iterative modified Newton-Raphson scheme is used for the solution of nonlinear problem. An energy criterion in terms of both forces and displacements is implemented,. The applicability and validity of this analytical model incorporating both geometrical and material nonlinearities is amply demonstrated through several numerical examples. The solution technique proposed in this paper has been shown to be successful in preventing the drifting of the solution in incremental process.link_to_subscribed_fulltex

MicroRNA-129-5p Regulates Glycolysis and Cell Proliferation by Targeting the Glucose Transporter SLC2A3 in Gastric Cancer Cells

Tumor cells increase their glucose consumption through aerobic glycolysis to manufacture the necessary biomass required for proliferation, commonly known as the Warburg effect. Accumulating evidences suggest that microRNAs (miRNAs) interact with their target genes and contribute to metabolic reprogramming in cancer cells. By integrating high-throughput screening data and the existing miRNA expression datasets, we explored the roles of candidate glycometabolism-regulating miRNAs in gastric cancer (GC). Subsequent investigation of the characterized miRNAs indicated that miR-129-5p inhibits glucose metabolism in GC cells. miRNA-129-5p directly targets the 3′-UTR of SLC2A3, thereby suppressing glucose consumption, lactate production, cellular ATP levels, and glucose uptake of GC cells. In addition, the PI3K-Akt and MAPK signaling pathways are involved in the effects of the miR-129-5p/SLC2A3 axis, regulating GC glucose metabolism and growth. These results reveal a novel role of the miR-129-5p/SLC2A3 axis in reprogramming the glycometabolism process in GC cells and indicate a potential therapeutic target for the treatment of this disease