Five Transcription Factors and FGF Pathway Inhibition Efficiently Induce Erythroid Differentiation in the Epiblast

SummaryPrimitive erythropoiesis follows a stereotypic developmental program of mesoderm ventralization and internalization, hemangioblast formation and migration, and erythroid lineage specification. Induction of erythropoiesis is inefficient in either ES/iPS cells in vitro or nonhemangioblast cell populations in vivo. Using the chick model, we report that epiblast cells can be directly and efficiently differentiated into the erythroid lineage by expressing five hematopoietic transcription regulators (SCL+LMO2+GATA2+LDB1+E2A) and inhibiting the FGF pathway. We show that these five genes are expressed with temporal specificity during normal erythropoiesis. Initiation of SCL and LMO2 expression requires FGF activity, whereas erythroid differentiation is enhanced by FGF inhibition. The lag between hematopoiesis and erythropoiesis is attributed to sequential coregulator expression and hemangioblast migration. Globin gene transcription can be ectopically and prematurely induced by manipulating the availability of these factors and the FGF pathway activity. We propose that similar approaches can be taken for efficient erythroid differentiation in vitro

Cis-Cotranscription of Two Beta Globin Genes during Chicken Primitive Hematopoiesis

Chicken beta globin locus contains four genes, two of which, rho and epsilon, are expressed from the earliest stage of primitive hematopoiesis. Here we show that the transcription of these two genes in the nucleus engages in “on/off” phases. During each “on” phase, cotranscription of rho and epsilon in cis is favored. We propose that these two chicken beta globin genes are transcribed not by competing for a transcription initiation complex, but in a cooperative way

The within-field and between-field dispersal of weedy rice by combine harvesters

International audienceAbstractWeedy rice (Oryza sativa L.) severely decreases the grain yield and profitability of rice is one of the most significant problems in the majority of rice fields worldwide. Few reports focus on the dispersal of weedy rice, especially how it rapidly spreads to large areas and long distances. Here, we quantify for the first time the within- and between-field dispersal of weedy rice associated with combine harvesting operations. We randomly sampled 31 combine harvesters to determine where and how much weedy rice seeds remained on the machines at three locations in Jiangsu Province, China. Based on the sampling results, the field area over which weedy rice seeds were retained on the combine harvester during harvesting was estimated to assess the within-field dispersibility of weedy rice seeds remaining in the harvesters. A tracking experiment was also carried out by tracing the distribution of weedy rice seeds along harvest trails, to estimate the dispersal of weedy rice seeds within the field being harvested. Weedy rice seeds remained in the harvest pocket, on the pedrail, and the metal plate of the combine harvester. On average, more than 5000 weedy rice seeds which were 22.80% of remaining grains could potentially be transported into adjacent fields by the combine after each rice field infested with weedy rice had been harvested. Of the statistical models compared, a double exponential model simulating the variation in seed retention predicted that weedy rice seeds remaining on the metal plate could be dispersed over 6473.91 m2 or 3236.96 m into the next field during the harvesting operation. Within the field, the number of fallen weedy rice seeds and their dispersal distance were positively correlated to weedy rice panicle density with the combine dispersing most of seeds away from their mother plant thus creating new weed patches. Therefore, fields that were severely infested with weedy rice should be harvested cautiously and separately and seed remaining in a harvester should be avoided to prevent intra- and inter-field, and even cross-regional dispersal of weedy rice

Expression profiling of circulating non-red blood cells in embryonic blood

<p>Abstract</p> <p>Background</p> <p>In addition to erythrocytes, embryonic blood contains other differentiated cell lineages and potential progenitor or stem cells homed to changing niches as the embryo develops. Using chicken as a model system, we have isolated an enriched pool of circulating non red blood cells (nRBCs) from E4 and E6 embryos; a transition period when definitive hematopoietic lineages are being specified in the peri-aortic region.</p> <p>Results</p> <p>Transcriptome analysis of both nRBC and RBC enriched populations was performed using chicken Affymetrix gene expression arrays. Comparison of transcript profiles of these two populations, with verification by RT-PCR, reveals in nRBCs an expression signature indicative of hematopoietic stem cells (HSCs) and progenitor cells of myeloid and lymphoid lineages, as well as a number of previously undescribed genes possibly involved in progenitor and stem cell maintenance.</p> <p>Conclusion</p> <p>This data indicates that early circulating embryonic blood contains a full array of hematopoietic progenitors and stem cells. Future studies on their heterogeneity and differentiation potentials may provide a useful alternative to ES cells and perinatal blood.</p

Review on recent liquefied natural gas cold energy utilization in power generation cycles

Liquefied natural gas (LNG) needs to be gasified before supplied to the users, and considerable amount of cold energy, about 830 kJ/kg, will be released during this process. Recovery of LNG cold energy bears significance of energy-saving and environmental protection. Among the many ways of using LNG cold energy, power generation is the most effective and suitable one for large-scale applications. Many novel power generation cycles have been designed for utilizing LNG cold energy so far. This paper reviews the recent researches on LNG cold energy utilization in power generation, and discusses 15 novel power generation cycles utilizing LNG cold energy.Cited as: Yu, G., Jia, S., Dai, B. Review on recent liquefied natural gas cold energy utilization in power generation cycles. Advances in Geo-Energy Research, 2018, 2(1): 86-102, doi: 10.26804/ager.2018.01.0

Regulation of programmed cell death during neural induction in the chick embryo

To study early responses to neural inducing signals from the organizer (Hensen's node), a differential screen was performed in primitive streak stage chick embryos, comparing cells that had or had not been exposed to a node graft for 5 hours. Three of the genes isolated have been implicated in Programmed Cell Death (PCD): Defender Against Cell Death (Dad1), Polyubiquitin II (UbII) and Ferritin Heavy chain (fth1). We therefore explored the potential involvement of PCD in neural induction. Dad1, UbII and fth1 are expressed in partly overlapping domains during early neural plate development, along with the pro-apoptotic gene Cas9 and the death effector Cas3. Dad1 and UbII are induced by a node graft within 3 hours. TUNEL staining revealed that PCD is initially random, but both during normal development and following neural induction by a grafted node, it becomes concentrated at the border of the forming neural plate and anterior non-neural ectoderm and downregulated from the neural plate itself. PCD was observed in regions of Caspase expression that are free from Dad1, consistent with the known anti-apoptotic role of Dad1. However, gain- and loss-of-function of any of these genes had no detectable effect on cell identity or on neural plate development. This study reveals that early development of the neural plate is accompanied by induction of putative pro- and anti-apoptotic genes in distinct domains. We suggest that the neural plate is protected against apoptosis, confining cell death to its border and adjacent non-neural ectoderm