A comparison about the inhibitory effect of curcunmin and Avastin on the rat corneal neovascularization

AIM: To compare the inhibitory effect of curcunmin and Avastin on the rat corneal neovascularization(CNV), and approach the mechanism of the curcunmin's inhibition. METHODS: CNV was established in thirty SD rats by alkaline burning. Rats were divided equally to group A and group B at random. In group A, right eyes were experimental group A1, treated by 40μmol/L curcunmin solution, and left eyes were control group A2, treated by 0.09% sodium chloride. In group B, right eyes were experimental group group B1, treated by 5g/L avastin, and left eyes were control group B2, treated by 0.09% sodium chloride. Cornea and aqueous humor were collected by time spot. The capillary vessels were study, and the expressions of VEGF were detected by Enzyme-Linked immunosorbnent Assay(ELISA). RESULTS: No toxic effects of the drugs were found. The capillary vessels in experimental group were less than those of control group(P<0.01). No statistical different of the capillary vessels between two drugs were found. The expressions of VEGF in experimental group were less than those in control group(P<0.01). The expressions of VEGF in B1 group were less than in group A1. CONCLUSION: The inhibitory effect to CNV of curcunmin and avastin have no statistical different in the experiment, but curcunmin has the less inhibitory effect to the expressions of VEGF than avastin. Curcunmin may have other mechanism in the inhibitory action on CNV

2-(2,2-Dimethyl-2,3-dihydro-1-benzofuran-7-yl­oxy)-N-(o-tol­yl)acetamide

In the title compound, C19H21NO3, the dihedral angle between the mean planes of the two benzene rings is 38.13 (12)°. The furan ring adopts an envelope-like conformation with the C atom bonded to the dimethyl groups displaced by 0.356 (2) Å from the plane through the other four atoms. In the crystal, mol­ecules are linked into inversion dimers by weak C—H⋯O inter­molecular inter­actions

Administration of a novel penicillamine-bound membrane: a preventive and therapeutic treatment for abdominal adhesions

<p>Abstract</p> <p>Background</p> <p>Adhesions formation is a significant postsurgical complication. At present, there is no effective method for preventing adhesions formation <abbrgrp><abbr bid="B1">1</abbr></abbrgrp>, although barrier products such as Dextran (Dex) <abbrgrp><abbr bid="B2">2</abbr></abbrgrp> and sodium hyaluronate (SH) <abbrgrp><abbr bid="B3">3</abbr></abbrgrp> have proved the most clinically successful <abbrgrp><abbr bid="B4">4</abbr><abbr bid="B5">5</abbr><abbr bid="B6">6</abbr></abbrgrp>, This study is designed to investigate the preventive and therapeutic potential of a novel penicillamine-bound membrane for abdominal adhesions formation.</p> <p>Methods</p> <p>150 rats were involved in the present study. All animals were randomly divided into 6 groups (1 vehicle group and 5 test groups respectively treated with dextran, sodium hyaluronate, penicillamine, penicillamine-bound membrane or non-penicillamine-bound membrane). The occurrence, grade and score of abdominal adhesions were compared between the different groups. The breaking strength of incision was compared between the vehicle group and the penicillamine, membrane with/without penicillamine - treated groups. Expression of collagen type I was compared between the vehicle and penicillamine-treated group. The occurrence of adhesions was compared between the Dextran (Dex), sodium hyaluronate (SH), penicillamine-treated group and membrane with or without penicillamine- treated groups.</p> <p>Results</p> <p>Penicillamine and penicillamine-bound membrane had significant preventive effects on abdominal adhesions formation, better than dextran, sodium hyaluronate and non-penicillamine-bound membrane. However, neither of them influenced incision healing, although they insignificantly decreased the breaking strength of the incision. Penicillamine-bound membrane, which can be loaded locally and more efficaciously, shows greater advantages than penicillamine.</p> <p>Conclusions</p> <p>Penicillamine-bound membrane can be applied as an effective therapeutic intervention for abdominal adhesions with inconsequential side effects.</p

Throughput Maximization for Intelligent Refracting Surface Assisted mmWave High-Speed Train Communications

With the increasing demands from passengers for data-intensive services, millimeter-wave (mmWave) communication is considered as an effective technique to release the transmission pressure on high speed train (HST) networks. However, mmWave signals ncounter severe losses when passing through the carriage, which decreases the quality of services on board. In this paper, we investigate an intelligent refracting surface (IRS)-assisted HST communication system. Herein, an IRS is deployed on the train window to dynamically reconfigure the propagation environment, and a hybrid time division multiple access-nonorthogonal multiple access scheme is leveraged for interference mitigation. We aim to maximize the overall throughput while taking into account the constraints imposed by base station beamforming, IRS discrete phase shifts and transmit power. To obtain a practical solution, we employ an alternating optimization method and propose a two-stage algorithm. In the first stage, the successive convex approximation method and branch and bound algorithm are leveraged for IRS phase shift design. In the second stage, the Lagrangian multiplier method is utilized for power allocation. Simulation results demonstrate the benefits of IRS adoption and power allocation for throughput improvement in mmWave HST networks.Comment: 13 pages, 7 figures, IEEE Internet of Things Journa

Nuclear factor κB controls acetylcholine receptor clustering at the neuromuscular junction

At the vertebrate neuromuscular junction (NMJ), acetylcholine receptor (AChR) clustering is stimulated by motor neuron-derived glycoprotein Agrin and requires a number of intracellular signal or structural proteins, including AChR-associated scaffold protein Rapsyn. Here, we report a role of nuclear factor κB (NF-κB), a well known transcription factor involved in a variety of immune responses, in regulating AChR clustering at the NMJ. We found that downregulating the expression of RelA/p65 subunit of NF-κB or inhibiting NF-κB activity by overexpression of mutated form of IκB (inhibitor κB), which is resistant to proteolytic degradation and thus constitutively keeps NF-κB inactive in the cytoplasma, impeded the formation of AChR clusters in cultured C2C12 muscle cells stimulated by Agrin. In contrast, overexpression of RelA/p65 promoted AChR clustering. Furthermore, we investigated the mechanism by which NF-κB regulates AChR clustering. Interestingly, we found that downregulating the expression of RelA/p65 caused a marked reduction in the protein and mRNA level of Rapsyn and upregulation of RelA/p65 enhanced Rapsyn promoter activity. Mutation of NF-κB binding site on Rapsyn promoter prevented responsiveness to RelA/p65 regulation. Moreover, forced expression of Rapsyn in RelA/p65 downregulated muscle cells partially rescued AChR clusters, suggesting that NF-κB regulates AChR clustering, at least partially through the transcriptional regulation of Rapsyn. In line with this notion, genetic ablation of RelA/p65 selectively in the skeletal muscle caused a reduction of AChR density at the NMJ and a decrease in the level of Rapsyn. Thus, NF-κB signaling controls AChR clustering through transcriptional regulation of synaptic protein Rapsyn. Copyrigh

Biodegradable Cellulose Film Prepared from Banana Pseudo-Stem Using an Ionic Liquid for Mango Preservation

The excessive use and disposal of plastic packaging materials have drawn increasing concerns from the society because of the detrimental effect on environment and ecosystems. As the most widely used fruit packing material, polyethylene (PE) film is not suitable for long-term preservation of some tropical fruits, such as mangos, due to its inferior gas permeability. Cellulose based film can be made from renewable resources and is biodegradable and environmental-friendly, which makes it a promising alternative to PE as a packaging material. In this study, cellulose film synthesized from delignified banana stem fibers via an ionic liquid 1-Allyl-3-methylimidazolium chloride ([AMIm][Cl]) were evaluated as packing material for mangos preservation. The moisture vapor transmission rate and gas transmission rate of the synthesized cellulose film were 1,969.1 g/(m2⋅24 h) and 10,015.4 ml/(m2⋅24 h), respectively, which are significantly higher than those of commercial PE films. The high permeability is beneficial to the release of ethylene so that contribute to extend fruit ripening period. As a result, cellulose film packaging significantly decreased the disease and color indexes of mangos, while prolonged the storage and shelf life of marketable fruits. In addition, the cellulose film was decomposed in soils in 4 weeks, indicating an excellent biodegradability as compared to the PE plastic film