Description of Campanella hanchuanensis p. nov. (Ciliophora, Peritrichida): general morphology and isozyme pattern

Living and stained specimens of Campanella hanchuanensis sp. nov., collected near Hanchuan Power Plant, Hubei Province, China, were studied under light microscopy. Infraciliature was stained using a protargol staining technique. There is no bridge between concentric silver lines. The aboral ciliary wreath consists of 6 lines of basal bodies, with the middle 4 lines compacted and one lateral line each side separated. The other characters are similar to or the same as that described in Campanella umbellaria. The esterase isozyme was analyzed by micro-isoelectrofocussing. Campanella hanchuanensis sp. nov. has three bands of esterase near the cathode.Article信州大学理学部附属諏訪臨湖実験所報告 9: 87-94(1995)departmental bulletin pape

Urotricha armatus: drawing

Apresenta imagem microscópica do Urotricha armatus como ilustrado em Shen & Zhang (1990) Biomonitorando técnicas usadas na água fresca Microbiota, Arquitetura chinesa e Impressa construídaComponente Curricular::Educação Superior::Ciências Biológicas::Microbiologi

Urotricha armatus: drawing

Apresenta imagem microscópica do Urotricha armatus como ilustrado em Shen & Zhang (1990) Biomonitorando técnicas usadas na água fresca Microbiota, Arquitetura chinesa e Impressa construídaComponente Curricular::Educação Superior::Ciências Biológicas::Microbiologi

Genetic relationships of five peritrichous ciliates inferred from inter simple sequence repeat (ISSR) molecular markers

In this study, ISSR (inter simple sequence repeat) molecular markers were used to clarify genetic relationships of five peritrichous ciliates: Carchesium polypinum, Epistylis chrysemydis, E. plicatilis, E. urceolata and Vorticella campanula.From 34 primers, 13 polymorphic primers were selected and tested. The genetic distance values going from 0.6667 to 1. 0000 among the five species show the ISSR-PCR method has a high resolution. In the phylogenetic tree constructed with the unweighted pair group method using arithmetic averages (UPGMA), V. campanula was separated from other species firstly; C.polypinum and E. chrysemydis were closely related; in the group of Epistylis species, E. plicatilis and E. urceolata were first clustered together and then grouped with E. chrysemydis. These results were compared with the phylogenetic tree from the complete small subunit ribosomal RNA (SSrRNA) gene sequences. We found that: 1) ISSR primers can give the informative profiles in peritrichous ciliates; 2) C. polypinum has a nearer relation with Epistylis than V. campanula. The colonial or solitary status might be a more important phylogenetic character within peritrichs than the characters of stalk and the myoneme; 3) E.urceolata shared closer relationship with E. plicatilis than E. chrysemydis. The hollow nature of the stalk is a useful phylogenetic and taxonomic character in the genus Epistylis. This study demonstrated that ISSR-PCR method was a new useful method to reveal the relationships among related or similar species in ciliates

Genetic relationships of five peritrichous ciliates inferred from inter simple sequence repeat (ISSR) molecular markers

In this study, ISSR (inter simple sequence repeat) molecular markers were used to clarify genetic relationships of five peritrichous ciliates: Carchesium polypinum, Epistylis chrysemydis, E. plicatilis, E. urceolata and Vorticella campanula.From 34 primers, 13 polymorphic primers were selected and tested. The genetic distance values going from 0.6667 to 1. 0000 among the five species show the ISSR-PCR method has a high resolution. In the phylogenetic tree constructed with the unweighted pair group method using arithmetic averages (UPGMA), V. campanula was separated from other species firstly; C.polypinum and E. chrysemydis were closely related; in the group of Epistylis species, E. plicatilis and E. urceolata were first clustered together and then grouped with E. chrysemydis. These results were compared with the phylogenetic tree from the complete small subunit ribosomal RNA (SSrRNA) gene sequences. We found that: 1) ISSR primers can give the informative profiles in peritrichous ciliates; 2) C. polypinum has a nearer relation with Epistylis than V. campanula. The colonial or solitary status might be a more important phylogenetic character within peritrichs than the characters of stalk and the myoneme; 3) E.urceolata shared closer relationship with E. plicatilis than E. chrysemydis. The hollow nature of the stalk is a useful phylogenetic and taxonomic character in the genus Epistylis. This study demonstrated that ISSR-PCR method was a new useful method to reveal the relationships among related or similar species in ciliates

(Protozoa: Rhizopoda: Arcellinida: Difflugiidae) from China

Summary. The freshwater testate amoeba Difflugia tuberspinifera Hu et al. 1997 collected from pond and lake in China, is investigated by light and scanning electron microscopy. This little known taxon is redescribed and its morphology, biometry and ecology are supplied. After carefully comparison with other six similar species including Difflugia bartoši Štìpánek, D. corona Wallich, D. corona cashi Deflandre, D. corona tuberculata Vucetich, D. muriformis Gauthier-Liévre et Thomas and Netzelia tuberculata (Wallich) Netzal we believe that the sub-spherical to spherical shell, the mulberry-shaped appearance, the 7-10 apertural tooth-like structures, the short collar and the conical spines numbering from 4 to 8 at the upper equatorial region in D. tuberspinifera set it apart from other species. Besides, statistical analysis indicates that D. tuberspinifera is a size-monomorphic species characterized by a main-size class and a small size range and the shell height is significant correlated with other morphometric characters at p < 0.05 excepting the number of aperture tooth-like structures and the number of spines. Moreover, D. tuberspinifera inhabits not only lotic but also lentic environment

Monitoring of river pollution

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