A multi-protein receptor-ligand complex underlies combinatorial dendrite guidance choices in C. elegans.

Ligand receptor interactions instruct axon guidance during development. How dendrites are guided to specific targets is less understood. The C. elegans PVD sensory neuron innervates muscle-skin interface with its elaborate dendritic branches. Here, we found that LECT-2, the ortholog of leukocyte cell-derived chemotaxin-2 (LECT2), is secreted from the muscles and required for muscle innervation by PVD. Mosaic analyses showed that LECT-2 acted locally to guide the growth of terminal branches. Ectopic expression of LECT-2 from seam cells is sufficient to redirect the PVD dendrites onto seam cells. LECT-2 functions in a multi-protein receptor-ligand complex that also contains two transmembrane ligands on the skin, SAX-7/L1CAM and MNR-1, and the neuronal transmembrane receptor DMA-1. LECT-2 greatly enhances the binding between SAX-7, MNR-1 and DMA-1. The activation of DMA-1 strictly requires all three ligands, which establishes a combinatorial code to precisely target and pattern dendritic arbors

Marine Debris Detection in Satellite Surveillance using Attention Mechanisms

Marine debris is an important issue for environmental protection, but current methods for locating marine debris are yet limited. In order to achieve higher efficiency and wider applicability in the localization of Marine debris, this study tries to combine the instance segmentation of YOLOv7 with different attention mechanisms and explores the best model. By utilizing a labelled dataset consisting of satellite images containing ocean debris, we examined three attentional models including lightweight coordinate attention, CBAM (combining spatial and channel focus), and bottleneck transformer (based on self-attention). Box detection assessment revealed that CBAM achieved the best outcome (F1 score of 77%) compared to coordinate attention (F1 score of 71%) and YOLOv7/bottleneck transformer (both F1 scores around 66%). Mask evaluation showed CBAM again leading with an F1 score of 73%, whereas coordinate attention and YOLOv7 had comparable performances (around F1 score of 68%/69%) and bottleneck transformer lagged behind at F1 score of 56%. These findings suggest that CBAM offers optimal suitability for detecting marine debris. However, it should be noted that the bottleneck transformer detected some areas missed by manual annotation and displayed better mask precision for larger debris pieces, signifying potentially superior practical performance

The selective use of gaze in automatic speech recognition

The performance of automatic speech recognition (ASR) degrades significantly in natural environments compared to in laboratory assessments. Being a major source of interference, acoustic noise affects speech intelligibility during the ASR process. There are two main problems caused by the acoustic noise. The first is the speech signal contamination. The second is the speakers' vocal and non-vocal behavioural changes. These phenomena elicit mismatch between the ASR training and recognition conditions, which leads to considerable performance degradation. To improve noise-robustness, exploiting prior knowledge of the acoustic noise in speech enhancement, feature extraction and recognition models are popular approaches. An alternative approach presented in this thesis is to introduce eye gaze as an extra modality. Eye gaze behaviours have roles in interaction and contain information about cognition and visual attention; not all behaviours are relevant to speech. Therefore, gaze behaviours are used selectively to improve ASR performance. This is achieved by inference procedures using noise-dependant models of gaze behaviours and their temporal and semantic relationship with speech. `Selective gaze-contingent ASR' systems are proposed and evaluated on a corpus of eye movement and related speech in different clean, noisy environments. The best performing systems utilise both acoustic and language model adaptation

Nanodelivery of a functional membrane receptor to manipulate cellular phenotype.

Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging and produce unwanted side effects. Here we develop and validate a nanodelivery approach to transfer in vitro synthesized, functional membrane receptors into the plasma membrane of living cells. Using β2-adrenergic receptor (β2AR), a prototypical G-protein coupled receptor, as an example, we demonstrated efficient incorporation of a full-length β2AR into a variety of mammalian cells, which imparts pharmacologic control over cellular signaling and affects cellular phenotype in an ex-vivo wound-healing model. Our approach for nanodelivery of functional membrane receptors expands the current toolkit for DNA and RNA-free manipulation of cellular function. We expect this approach to be readily applicable to the synthesis and nanodelivery of other types of GPCRs and membrane receptors, opening new doors for therapeutic development at the intersection between synthetic biology and nanomedicine

Functionally distinct and selectively phosphorylated GPCR subpopulations co-exist in a single cell.

G protein-coupled receptors (GPCRs) transduce pleiotropic intracellular signals in a broad range of physiological responses and disease states. Activated GPCRs can undergo agonist-induced phosphorylation by G protein receptor kinases (GRKs) and second messenger-dependent protein kinases such as protein kinase A (PKA). Here, we characterize spatially segregated subpopulations of β2-adrenergic receptor (β2AR) undergoing selective phosphorylation by GRKs or PKA in a single cell. GRKs primarily label monomeric β2ARs that undergo endocytosis, whereas PKA modifies dimeric β2ARs that remain at the cell surface. In hippocampal neurons, PKA-phosphorylated β2ARs are enriched in dendrites, whereas GRK-phosphorylated β2ARs accumulate in soma, being excluded from dendrites in a neuron maturation-dependent manner. Moreover, we show that PKA-phosphorylated β2ARs are necessary to augment the activity of L-type calcium channel. Collectively, these findings provide evidence that functionally distinct subpopulations of this prototypical GPCR exist in a single cell

Simulation study on effect of chassis water cooling on solidification of eleven tons flat steel ingot

In this paper, the solidification process of eleven tons flat steel ingot is simulated by the finite element analysis software PROCAST, and the solidification state of the ingot with and without chassis is analyzed and compared. The results show that the forced cooling chassis makes low temperature area of the bottom ingot enlarged. And it has little influence on the temperature field and the solidification speed of the upper ingot. For the small flat steel ingot, the forced cooling chassis will deteriorate the shrinkage