Biochemical characterization of glucosaminylmuramyldipeptide binding sites of murine macrophages

AbstractBy using radioligand analysis, murine peritoneal macrophages were shown to express several hundred high-affinity cell surface GMDP-binding sites (Ka 350 pM). Photoaffinity labeling followed by SDS-PAGE enabled us to identify 32–34 and 38 kDa proteins inside these cells that bound GMDP specifically