19 research outputs found
Temperatura de polimerização da resina acrílica odontológica na medula espinhal de ratos Wistar
Aspectos epidemiológicos, clínicos e anatomopatológicos da intoxicação por aceturato de diminazeno em cães
Relevant aspects of golden retriever muscular dystrophy for the study of Duchenne muscular dystrophy in humans
Degenerative Myelopathy in German Shepherd Dogs: An Association with Mucosal Piochemical Changes and Bacterial Overgrowth in the Small Intestine
Degenerative Myelopathy in German Shepherd Dogs: An Association with Mucosal Biochemical Changes and Bacterial Overgrowth in the Small Intestine
Alternative dystrophin gene transcripts in golden retriever muscular dystrophy
Golden retriever muscular dystrophy (GRMD), the canine model of Duchenne muscular dystrophy (DMD), is caused by a splice site mutation in the dystrophin gene. This mutation predicts a premature termination codon in exon 8 and a peptide that is 5% the size of normal dystrophin. Western blot analysis of skeletal muscle from GRMD dogs reveals a slightly truncated 390-kD protein that is approximately 91% the size of normal dystrophin. This 390-kD dystrophin suggests that GRMD dogs, like some DMD patients, employ a mechanism to overcome their predicted frameshift. Reverse-transcriptase polymerase chain reaction on GRMD muscle has revealed two in-frame dystrophin transcripts which lack either exons 3–9 or exons 5–12. Both transcripts could be translated into a dystrophin protein of approximately 390 kD. An understanding of how truncated dystrophin is produced in GRMD may allow this mechanism to be manipulated toward a potential therapy for DMD