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Not AvailableA new epithelial cell line, WAF was developed from caudal fin of freshwater shark, Wallago attu. The cell line was optimally maintained at 28 _C in Leibovitz-15 (L-15) medium supplemented with 20 % fetal bovine serum. The cell line was characterized by various cytogenetic and molecular markers. The cytogenetic analysis revealed a diploid count of 86 chromosomes at different passages. The origin of the cell lines was confirmed by the amplification of 547 and 654 bp sequences of 16S rRNA and cytochrome oxidase subunit I genes of mitochondrial DNA, respectively. WAF cells were characterized for their growth characteristics at different temperature and serum concentration. Epithelial morphology of the cell line was confirmed using immunocytochemistry. Further cell plating efficiency, transfection efficiency and viability of cryopreserved WAF cells was also determined. ytotoxicity and genotoxicity assessment of cadmium salts on WAF cells by MTT, NR and comet assay illustrated the utility of this cell line as an in vitro model for aquatic toxicological studies. The cell line will be further useful for studying oxidative stress markers against aquatic pollutants.Department of Biotechnology, Government of Indi

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Not AvailableThe occurrence of Turnip mosaic virus (TuMV) in cole crops (Brassica spp) grown in Basar, Arunachal Pradesh, India was confirmed by symptomatol- ogy, transmission electron microscopy, reverse transcrip- tion-polymerase chain reaction and partial characterization of cytoplasmic inclusion protein and coat protein (CP) domains. Phylogenetic analysis of the partial CP sequences of the new TuMV isolates from Indian mustard (AR- IndM), broad leaved mustard (AR-BrLM) and broccoli (AR-Broc) revealed their closest relationship with mem- bers of the World-B genogroup of TuMV. This is the first molecular evidence of TuMV infection in Brassica spp from IndiaNot Availabl