Problems of operational control of physical and chemical environmental factors in residential areas of Russian settlements

The formation of human life conditions in residential areas depends on the state of ecological balance in these territories, which, in turn, relays the influence of various natural and anthropogenic environmental factors. From a practical point of view, this influence is increasingly becoming cumulative or even complex. The article discusses the need to create a system of integrated (aggregate) monitoring of residential areas, providing visualization of predictive estimates of the spread of pollution. To solve such a problem, it is necessary to develop a theoretical model (concept) of complex (aggregate, generalized) monitoring of the ecological situation. This approach is based on the use of the principle of combining data on the nature and parameters of the main air pollutants, infra- and ultrasonic, as well as electromagnetic radiation, vibrations, meteorological parameters and the structure of landscape features of the zone. Modern automated monitoring systems of natural and technogenic areas are considered. Their advantages and disadvantages are noted. It is concluded that a system is necessary to develop, which solves a wider range of tasks

Observing temporary changes in gait using a mobile phone

The purpose of the article is to analyze temporary changes in human gait, the parameters of which are recorded using the accelerometer of a mobile phone in everyday use. The work presents trends in the field of personalized medicine and monitoring the health of employees in the manufacturing sector based on wearable and mobile devices. The features of collecting and processing data on a person’s gait using a mobile phone are presented, which act as a comprehensive assessment of human health parameters. The results of a year-long research of changes in human gait are presented. Daily, weekly and annual trends are identified. The research analyzed mobile phone accelerometer time series representing double steps during gait. When constructing trends, the maximum value of the cross-correlation function of data segments was used. Data segmentation was carried out according to the length of the double step. Based on the results of the research, an assumption is made about the possibility of recording individual characteristics of the functioning of the human musculoskeletal system using a mobile phone and conducting further research in order to accumulate statistical material and identify the relationship between a person’s lifestyle and changes in gait parameters

Problems of operational control of physical and chemical environmental factors in residential areas of Russian settlements

The formation of human life conditions in residential areas depends on the state of ecological balance in these territories, which, in turn, relays the influence of various natural and anthropogenic environmental factors. From a practical point of view, this influence is increasingly becoming cumulative or even complex. The article discusses the need to create a system of integrated (aggregate) monitoring of residential areas, providing visualization of predictive estimates of the spread of pollution. To solve such a problem, it is necessary to develop a theoretical model (concept) of complex (aggregate, generalized) monitoring of the ecological situation. This approach is based on the use of the principle of combining data on the nature and parameters of the main air pollutants, infra- and ultrasonic, as well as electromagnetic radiation, vibrations, meteorological parameters and the structure of landscape features of the zone. Modern automated monitoring systems of natural and technogenic areas are considered. Their advantages and disadvantages are noted. It is concluded that a system is necessary to develop, which solves a wider range of tasks

The Role of Phospholipase Activity of Peroxiredoxin 6 in Its Transmembrane Transport and Protective Properties

Peroxiredoxin 6 (Prdx6) is a multifunctional eukaryotic antioxidant enzyme. Mammalian Prdx6 possesses peroxidase activity against a wide range of organic and inorganic hydroperoxides, as well as exhibits phospholipase A2 (aiPLA2) activity, which plays an important role in the reduction of oxidized phospholipids and cell membrane remodeling. Exogenous Prdx6 has recently been shown to be able to penetrate inside the cell. We hypothesized that this entry may be due to the phospholipase activity of Prdx6. Experiments using exogenous Prdx6 in three cell lines (3T3, A549, RAW 264.7) demonstrated that it is the phospholipase activity that promotes its penetration into the cell. Overoxidation of Prdx6 led to a suppression of the peroxidase activity and a 3-to-4-fold growth of aiPLA2, which enhanced the efficiency of its transmembrane transport into the cells by up to 15 times. A mutant form of Prdx6-S32A with an inactivated phospholipase center turned out to be unable to enter the cells in both the reduced and oxidized state of the peroxidase active center. Previously, we have shown that exogenous Prdx6 has a significant radioprotective action. However, the role of phospholipase activity in the radioprotective effects of Prdx6 remained unstudied. Trials with the mutant Prdx6-S32A form, with the use of a total irradiation model in mice, showed a nearly 50% reduction of the radioprotective effect upon aiPLA2 loss. Such a significant decrease in the radioprotective action may be due to the inability of Prdx6-S32A to penetrate animal cells, which prevents its reduction by the natural intracellular reducing agent glutathione S-transferase (πGST) and lowers the efficiency of elimination of peroxides formed from the effect of ionizing radiation. Thus, phospholipase activity may play an important role in the reduction of oxidized Prdx6 and manifestation of its antioxidant properties

Peroxiredoxin 6 Applied after Exposure Attenuates Damaging Effects of X-ray Radiation in 3T3 Mouse Fibroblasts

Although many different classes of antioxidants have been evaluated as radioprotectors, none of them are in widespread clinical use because of their low efficiency. The goal of our study was to evaluate the potential of the antioxidant protein peroxiredoxin 6 (Prdx6) to increase the radioresistance of 3T3 fibroblasts when Prdx6 was applied after exposure to 6 Gy X-ray. In the present study, we analyzed the mRNA expression profiles of genes associated with proliferation, apoptosis, cellular stress, senescence, and the production of corresponding proteins from biological samples after exposure of 3T3 cells to X-ray radiation and application of Prdx6. Our results suggested that Prdx6 treatment normalized p53 and NF-κB/p65 expression, p21 levels, DNA repair-associated genes (XRCC4, XRCC5, H2AX, Apex1), TLR expression, cytokine production (TNF-α and IL-6), and apoptosis, as evidenced by decreased caspase 3 level in irradiated 3T3 cells. In addition, Prdx6 treatment reduced senescence, as evidenced by the decreased percentage of SA-β-Gal positive cells in cultured 3T3 fibroblasts. Importantly, the activity of the NRF2 gene, an important regulator of the antioxidant cellular machinery, was completely suppressed by irradiation but was restored by post-irradiation Prdx6 treatment. These data support the radioprotective therapeutic efficacy of Prdx6