ANTIDIABETIC EFFECTS OF A SERIES OF CURCUMIN PYRAZOLES IN-VITRO

Objective: To investigate the antidiabetic potential of a series of curcumin pyrazole derivatives in-vitro.Methods: The in-vitro antidiabetic ability of curcumin pyrazole derivatives (3a-3e) was evaluated by the inhibition studies of the digestive enzymes;including alpha-amylase, rat intestinal alpha-glucosidase, and sucrase. The glucose lowering effect of the compounds was further assessed by theglucose uptake assay using a porcine diaphragm.Results: The curcumin pyrazole derivatives (3a-3e) showed a prominent inhibition of the enzymes studied. Compounds 3a and 3b were found tobe more pronounced in their hypoglycemic effects and 3b exhibited the maximum enzyme inhibitory activity. The glucose uptake through porcinediaphragm was extensively promoted by the compounds 3a and 3b than the other compounds in the series.Conclusion: The results suggest that the pyrazole derivatives of curcumin 3a and 3b have potent hypoglycemic properties. These compounds in theseries of curcumin pyrazole derivatives (3a-3e) can be selected for further in-vitro and in vivo studies.Keywords: Diaphragm, Insulin, Hypoglycemic, Enzyme inhibition

IN VITRO ANTICANCER AND ANTI-LIPOXYGENASE ACTIVITIES OF CHIA SEED OIL AND ITS BLENDS WITH SELECTED VEGETABLE OILS

  Objective: To investigate the anticancer and anti-inflammatory potential of chia seed oil (CSO) and its blends in vitro.Methods: The in vitro cancer cytotoxic properties ability of CSO and its blends was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) and trypan blue assays using major cell lines including chronic myelogenous leukemia, HeLa and MCF-7 cells. The anti-inflammatory effect of the CSO and its blends was studied by assessing the anti-lipoxygenase (LOX) activity in vitro.Results: Incubation with CSO alone significantly inhibited the LOX activity. In the combination groups, CSO slightly increased the inhibition among blends of soybean and palmolein oils. In addition, CSO alone and in blends demonstrated potent and differential anticancer activity in MTT and trypan blue assays.Conclusion: Our results pave the way for the conclusion that CSO alone or in combination with the vegetable oils proves to be a healthy synergistic supplement or an adjuvant for the current diet. The supplementation with CSO is suggested for the modern lifestyle to delay or prevent the incidence of degenerative disorders