Phase Transition of Finite Size Quark Droplets with Isospin Chemical Potential in the Nanbu--Jona-Lasinio Model

Making use of the NJL model and the multiple reflection expansion pproximation, we study the phase transition of the finite size droplet with u and d quarks. We find that the dynamical masses of u, d quarks are different, and the chiral symmetry can be restored at different critical radii for u, d quark. It rovides a clue to understand the effective nucleon mass splitting in nuclear matter. Meanwhile, it shows that the maximal isospin chemical potential at zero temperature is much smaller than the mass of pion in free space.Comment: 12 pages, 3 figures. To appear in Physical Review

Shear viscosity of quark-gluon plasma at finite temperature and chemical potential and QCD phase transitions

We explore the shear viscosity of quark-gluon plasma (QGP) in the full QCD phase diagram within the framework of kinetic theory with the relaxation time approximation based on the $2 \leftrightarrow 2$ elastic scatterings of quark quasiparticles. The temperature and chemical potential dependent masses of particles, including $u, d, s$ quarks, their antiparticles, and exchanged mesons, are calculated in the Polyakov-loop extended Nambu--Jona Lasinio (PNJL) model. The results indicate that, at small chemical potential, the value of $\eta/s$ has a minimum near the Mott dissociation of mesons and increases rapidly in the lower-temperature side of the chiral crossover phase transition. At large chemical potential (high density), $\eta/s$ in the QGP phase is dominated by the temperature, and the value of $\eta/s$ is greatly enhanced at lower temperature. At intermediate temperature and chemical potential near the QCD phase transition, the situation is relatively complicated. The behavior of $\eta/s$ is influenced by the competition between temperature, density effect, and QCD phase transition.Comment: 12pages, 10 figure

HIF-1α Contributes to Hypoxia-induced Invasion and Metastasis of Esophageal Carcinoma via Inhibiting E-cadherin and Promoting MMP-2 Expression

Hypoxia-inducible factor-1α (HIF-1α) has been found to enhance tumor invasion and metastasis, but no study has reported its action in esophageal carcinoma. The goal of this study was to explore the probable mechanism of HIF-1α in the invasion and metastasis of esophageal carcinoma Eca109 cells in vitro and in vivo. mRNA and protein expression of HIF-1α, E-cadherin and matrix metalloproteinase-2 (MMP-2) under hypoxia were detected by RT-PCR and Western blotting. The effects of silencing HIF-1α on E-cadherin, MMP-2 mRNA and protein expression under hypoxia or normoxia were detected by RT-PCR and Western blotting, respectively. The invasive ability of Eca109 cells was tested using a transwell chambers. We established an Eca109-implanted tumor model and observed tumor growth and lymph node metastasis. The expression of HIF-1α, E-cadherin and MMP-2 in xenograft tumors was detected by Western blotting. After exposure to hypoxia, HIF-1α protein was up-regulated, both mRNA and protein levels of E-cadherin were down-regulated and MMP-2 was up-regulated, while HIF-1α mRNA showed no significant change. SiRNA could block HIF-1α effectively, increase E-cadherin expression and inhibit MMP-2 expression. The number of invading cells decreased after HIF-1α was silenced. Meanwhile, the tumor volume was much smaller, and the metastatic rate of lymph nodes and the positive rate were lower in vivo. Our observations suggest that HIF-1α inhibition might be an effective strategy to weaken invasion and metastasis in the esophageal carcinoma Eca109 cell line

Quantitative measurement of cell membrane receptor internalization by the nanoluciferase reporter: Using the G protein-coupled receptor RXFP3 as a model

AbstractNanoluciferase (NanoLuc) is a newly developed small luciferase reporter with the brightest bioluminescence to date. In the present work, we developed NanoLuc as a sensitive bioluminescent reporter to measure quantitatively the internalization of cell membrane receptors, based on the pH dependence of the reporter activity. The G protein-coupled receptor RXFP3, the cognate receptor of relaxin-3/INSL7, was used as a model receptor. We first generated stable HEK293T cells that inducibly coexpressed a C-terminally NanoLuc-tagged human RXFP3 and a C-terminally enhanced green fluorescent protein (EGFP)-tagged human RXFP3. The C-terminal EGFP-tag and NanoLuc-tag had no detrimental effects on the ligand-binding potency and intracellular trafficking of RXFP3. Based on the fluorescence of the tagged EGFP reporter, the ligand-induced RXFP3 internalization was visualized directly under a fluorescence microscope. Based on the bioluminescence of the tagged NanoLuc reporter, the ligand-induced RXFP3 internalization was measured quantitatively by a convenient bioluminescent assay. Coexpression of an EGFP-tagged inactive [E141R]RXFP3 had no detrimental effect on the ligand-binding potency and ligand-induced internalization of the NanoLuc-tagged wild-type RXFP3, suggesting that the mutant RXFP3 and wild-type RXFP3 worked independently. The present bioluminescent internalization assay could be extended to other G protein-coupled receptors and other cell membrane receptors to study ligand–receptor and receptor–receptor interactions

Twofold Structured Features-Based Siamese Network for Infrared Target Tracking

Nowadays, infrared target tracking has been a critical technology in the field of computer vision and has many applications, such as motion analysis, pedestrian surveillance, intelligent detection, and so forth. Unfortunately, due to the lack of color, texture and other detailed information, tracking drift often occurs when the tracker encounters infrared targets that vary in size or shape. To address this issue, we present a twofold structured features-based Siamese network for infrared target tracking. First of all, in order to improve the discriminative capacity for infrared targets, a novel feature fusion network is proposed to fuse both shallow spatial information and deep semantic information into the extracted features in a comprehensive manner. Then, a multi-template update module based on template update mechanism is designed to effectively deal with interferences from target appearance changes which are prone to cause early tracking failures. Finally, both qualitative and quantitative experiments are carried out on VOT-TIR 2016 dataset, which demonstrates that our method achieves the balance of promising tracking performance and real-time tracking speed against other out-of-the-art trackers.Comment: 13 pages,9 figures,references adde