In vitro Microdialysis Sampling Collection of Volatile Organic Compounds (VOC\u27s), Dodecafluoropentane (DDFP) and Isoflurane

Death by stroke occurs every four minutes to human beings. Strokes cause necrosis within the tissue of the brain due to deprivation of oxygen. Perfluorocarbons have the ability to transport oxygen to tissue and in return decrease cell death. Dodecafluoropentane (DDFP) is a volatile fluorocarbon and collection in vivo can be a challenge since this compound evaporates at room temperature. There is currently not an efficient collection method in vivo for compounds that are volatile. Without a method to collect DDFP it is impossible to be approved for clinical use since exact concentrations of the drug within the body will be unknown. The current in vitro work demonstrates that microdialysis can collect volatile organic compounds, isoflurane (a standard inhalation anesthetic) and DDFP. Different perfusion fluids and flow rates were tested for optimal analyte collection through the microdialysis membrane. Instead of utilizing an aqueous perfusion fluid safflower oil and air was passed through the microdialysis probe. The perfusion fluid and flow rate of choice for isoflurane sampling was safflower oil at 0.5 µL/min, respectively. For DDFP there was no significance in flux between flow rates and air was a more suitable perfusate. Since oil was a potential candidate as a perfusion fluid through the microdialysis probe, the oil/air partition coefficients (KOil/Air) were calculated. KOil/Air was determined because the analyte in the air phase will partition into the oil phase that is being perfused through the microdialysis probe. The average calculated KOil/Air for Isoflurane and DDFP was 10.62 and 0.53, respectively. These values lead to isoflurane partitioning more into oil vs. air and DDFP partitioning more into air vs. oil. The new analytical method described here shows that VOC’s can be collected with microdialysis sampling technique and thus serves as a starting point for in vivo collections has been found

Gene expression profiling of lymphoblastoid cell lines from monozygotic twins discordant in severity of autism reveals differential regulation of neurologically relevant genes

BACKGROUND: The autism spectrum encompasses a set of complex multigenic developmental disorders that severely impact the development of language, non-verbal communication, and social skills, and are associated with odd, stereotyped, repetitive behavior and restricted interests. To date, diagnosis of these neurologically based disorders relies predominantly upon behavioral observations often prompted by delayed speech or aberrant behavior, and there are no known genes that can serve as definitive biomarkers for the disorders. RESULTS: Here we demonstrate, for the first time, that lymphoblastoid cell lines from monozygotic twins discordant with respect to severity of autism and/or language impairment exhibit differential gene expression patterns on DNA microarrays. Furthermore, we show that genes important to the development, structure, and/or function of the nervous system are among the most differentially expressed genes, and that many of these genes map closely in silico to chromosomal regions containing previously reported autism candidate genes or quantitative trait loci. CONCLUSION: Our results provide evidence that novel candidate genes for autism may be differentially expressed in lymphoid cell lines from individuals with autism spectrum disorders. This finding further suggests the possibility of developing a molecular screen for autism based on expressed biomarkers in peripheral blood lymphocytes, an easily accessible tissue. In addition, gene networks are identified that may play a role in the pathophysiology of autism

Practice transformations to optimize the delivery of HIV primary care in community healthcare settings in the United States: A program implementation study.

BackgroundThe United States HIV care workforce is shrinking, which could complicate service delivery to people living with HIV (PLWH). In this study, we examined the impact of practice transformations, defined as efficiencies in structures and delivery of care, on demonstration project sites within the Workforce Capacity Building Initiative, a Health Resources and Services Administration (HRSA) Ryan White HIV/AIDS Program Special Projects of National Significance (SPNS).Methods and findingsData were collected at 14 demonstration project sites in 7 states and the District of Columbia. Organizational assessments were completed at sites once before and 4 times after implementation. They captured 3 transformation approaches: maximizing the HIV care workforce (efforts to increase the number of existing healthcare workforce members involved in the care of PLWH), share-the-care (team-based care giving more responsibility to midlevel providers and staff), and enhancing client engagement in primary HIV care to reduce emergency and inpatient care (e.g., care coordination). We also obtained Ryan White HIV/AIDS Program Services Reports (RSRs) from sites for calendar years (CYs) 2014-2016, corresponding to before, during, and after transformation. The RSR include data on client retention in HIV care, prescription of antiretroviral therapy (ART), and viral suppression. We used generalized estimating equation (GEE) models to analyze changes among sites implementing each practice transformation approach. The demonstration projects had a mean of 18.5 prescribing providers (SD = 23.5). They reported data on more than 13,500 clients per year (mean = 969/site, SD = 1,351). Demographic characteristics remained similar over time. In 2014, a majority of clients were male (71% versus 28% female and 0.2% transgender), with a mean age of 47 (interquartile range [IQR] 37-54). Racial/ethnic characteristics (48% African American, 31% Hispanic/Latino, 14% white) and HIV risk varied (31% men who have sex with men; 31% heterosexual men and women; 7% injection drug use). A substantial minority was on Medicaid (41%). Across sites, there was significant uptake in practices consistent with maximizing the HIV care workforce (18% increase, p < 0.001), share-the-care (25% increase, p < 0.001), and facilitating patient engagement in HIV primary care (13% increase, p < 0.001). There were also significant improvements over time in retention in HIV care (adjusted odds ratio [aOR] = 1.03; 95% confidence interval [CI] 1.02-1.04; p < 0.001), ART prescription levels (aOR = 1.01; 95% CI 1.00-1.01; p < 0.001), and viral suppression (aOR = 1.03; 95% CI 1.02-1.04; p < 0.001). All outcomes improved at sites that implemented transformations to maximize the HIV care workforce or improve client engagement. At sites that implemented share-the-care practices, only retention in care and viral suppression outcomes improved. Study limitations included use of demonstration project sites funded by the Ryan White HIV/AIDS Program (RWHAP), which tend to have better HIV outcomes than other US clinics; varying practice transformation designs; lack of a true control condition; and a potential Hawthorne effect because site teams were aware of the evaluation.ConclusionsIn this study, we found that practice transformations are a potential strategy for addressing anticipated workforce challenges among those providing care to PLWH. They hold the promise of optimizing the use of personnel and ensuring the delivery of care to all in need while potentially enhancing HIV care continuum outcomes

Oxidative instability of ionomers in hydroxide-exchange-membrane water electrolyzers

Hydroxide-exchange membrane (HEM) electrolyzers can produce green H2 with only earth-abundant catalysts and electrolyte-free (nominally pure) water feed, significantly decreasing system cost and complexity. However, HEM technology suffers from short lifetimes, attributed in part to poor stability of anion-exchange polymers used in the membrane and catalyst layers. We use electrochemical analysis and ex situ characterization techniques to study anion-exchange-polymer degradation in electrolyzers. Using multiple ionomers, catalyst-layer additives, and electrolyte feed, we show how anode-ionomer oxidation is the dominant degradation mechanism for all HEM-based electrolyzer cells tested. We find improved device stability using oxidation-resistant catalyst-layer binders and propose new design strategies for advanced ionomer and catalyst-layer development

The Path to Graduate School in Science and Engineering for Underrepresented Students of Color

Over the past decade, the numnber of Black, Hispanic, and American Indian/Alaska Native students attaining bachelor\u27s degrees in science and engineering fields has increased substantially. In 2004, 13.9% of all bachelor\u27s degrees in science and engineering fields were awarded to students from these three groups, up from 11.2% in 1995 (Hill & Green, 2007). Although Blacks, Hispanics, and American Indians continue to be underrepresented among bachelor\u27s degree recipients in science and engineering fields relative to their representation among all bachelor\u27s degree recipients (13.9% versus 16.9% in 2004, Hill & Green, 2007), these trends suggest that progress is being made

Testing Modality Affects Performance on the Santa Barbara Solids Test

Spatial ability is associated with performance in science, technology, engineering, and mathematics (STEM) disciplines and has been used to predict the likelihood of success in these fields (Wai, Lubinski, & Benbow, 2009). Classically, spatial ability has been assessed by tests that measure general factors of spatial ability. However, these factors may be limited in that they were not developed with individual differences or cognitive theories in mind (Cohen & Hegarty, 2012). Although traditional measures of spatial ability give insight into a person’s general spatial processing, Cohen and Hegarty (2012) point out the need for theoretically motivated spatial ability tests that specifically relate to STEM performance. There are numerous spatial ability measures in use by researchers, yet there is a need for reliable and valid spatial ability measures that are directly applicable to STEM fields. One new measure of spatial ability developed theoretically with individual differences in mind is the Santa Barbara Solids Test (SBST; Cohen & Hegarty, 2012). In the SBST, participants must imagine what the bisection of three-dimensional forms will be when cut by a two-dimensional plane. This bisection can be horizontal, vertical, or oblique, and the shape can be a simple or complex three-dimensional form. The spatial skills involved in imagining a cross-section of a form have been linked with performance in STEM courses, such as anatomy (Rochford, 1985), biology (Russell-Gebbett, 1985), geology (Kali & Orion, 1996), geometry (Pittalis & Christou, 2010), engineering (Duesbury & O’Neil, 1996), and skills such as reading x-rays and MRIs (Hegarty, Keehner, Cohen, Montello, & Lippa, 2007). The SBST has been validated with undergraduate students with a range of spatial ability scores (Cohen & Hegarty, 2012), but additional studies of the SBST are needed to replicate and expand on the findings of this promising new measure. For example, it is important to determine the effects of testing modality on performance to highlight a potential confound in future spatial ability studies. Although computerized assessments are common and offer many conveniences (e.g., fast scoring, fewer resources) compared to other testing modalities (e.g., paper-based testing), participants may experience higher perceived workload in computer-based assessments (Mayes, Sims, & Koonce, 2001) or perform differently on the same test in another modality (c.f. Noyes & Garland, 2008). The current study (n = 241) compares the SBST with a traditional measure of spatial ability, the Paper Folding Test (PFT; Ekstrom, French, Harman, & Dermen, 1976), in two testing modalities: 1) computer-based, and 2) paper-based. Results showed there was a correlation between the spatial ability measures, indicating both were tapping the same underlying construct. There was not a difference in performance between testing modalities for the PFT. However, there was a difference in performance based on testing modality for the SBST such that participants in the paper-based condition performed better than those in the computerized condition. The implications of these results are that testing modality should be a consideration for future studies involving the SBST

Identification of the Infrared Counterpart to a Newly Discovered X-ray Source in the Galactic Center

We present first results of a campaign to find and identify new compact objects in the Galactic Center. Selecting candidates from a combination of Chandra and 2MASS survey data, we search for accretion disk signatures via infrared spectroscopy. We have found the infrared counterpart to the Chandra source CXO J174536.1-285638, the spectrum of which has strong Br-gamma and HeI emission. The presence of CIII, NIII, and HeII indicate a binary system. We suspect that the system is some form of high-mass binary system, either a high-mass X-ray binary or a colliding wind binary.Comment: 11 pages, 6 figures, ApJ accepted, 200

Behavioral Health in Rural America: Understanding Citizen Perceptions and Willingness to Respond to Community Needs

Amid nationwide efforts to address behavioral health needs, rural communities often face unique challenges and a lack of resources. This study presents a bottom-up approach used by one rural community in the Midwest to respond to their needs regarding mental health and substance use. A survey instrument was developed from interviews with community stakeholders and disseminated in both online and paper formats. The survey sought to understand citizen perspectives regarding quality of life, barriers to treatment, and willingness to engage in efforts to address the community’s needs. Data from 1,303 respondents (71.5% women, 54.7% income \u3c$42,000) were analyzed using descriptive statistics and chi-square analyses. Results indicate that cost of treatment, shame, and lack of privacy were a barrier for most citizens’ treatment-seeking behavior. In addition, many citizens were willing to engage in strategies to address the community’s needs, including increased county spending, forming a neighborhood watch, and donating money. Differences associated with gender and income emerged across perceptions and willingness to support efforts. Implications for community efforts are discussed

Individual-Based Measure of Socio-Economic Disadvantage: Making Identification "Agile"

This project aimed to develop an individual-based measure of socio-economic disadvantage that can assist Australian universities to identify socio-economic disadvantage at the level of the individual student. A mixed-methods design was used. Phase 1 comprised an online questionnaire distributed to staff working in the area of widening participation and student support at each of the 42 universities in Australia (n = 256) and an online questionnaire for undergraduate students at three universities (one a member of the Group of Eight and the other two members of the Innovative Research Universities group – one a multi-campus metropolitan university and one a rural university) (n = 4,114). In Phase 2, five focus groups were conducted with staff and six with students to further explore the issues raised in Phase 1. Phase 3 consisted of a desk audit of university websites to provide data on the information publicly available to students experiencing socio-economic disadvantage. In the final phase, Phase 4, a possible approach to measuring disadvantage was evaluated through its presentation to students at two universities which had been part of Phase 1 (n = 91). The following factors were found to be indicators of socio-economic disadvantage. Whether the student: provides financially for their family; is the first in family to attend university; is experiencing financial hardship; and/or is in receipt of Youth Allowance, Austudy or ABSTUDY during Years 11 and/or 12 (or another Centrelink income and asset-tested entitlement) for a period of at least three months during Years 11 and/or 12 or equivalent. These were found to be questions that students were willing to answer and which would lead to effective and efficient identification

Temporal patterns of phytoplankton assemblages, size spectra and diversity during the wane of a Phaeocystis globosa spring bloom in hydrologically contrasted coastal waters

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