Fine Structure of the Sensilla and Immunolocalisation of Odorant Binding Proteins in the Cerci of the Migratory Locust, Locusta migratoria

Using light and electron microscopy (both scanning and transmission), we observed the presence of sensilla chaetica and hairs on the cerci of the migratory locust, Locusta migratoria L. (Orthoptera: Acrididae). Based on their fine structures, three types of sensilla chaetica were identified: long, medium, and short. Males presented significantly more numbers of medium and short sensilla chaetica than females (p<0.05). The other hairs can also be distinguished as long and short. Sensilla chaetica were mainly located on the distal parts of the cerci, while hairs were mostly found on the proximal parts. Several dendritic branches, enveloped by a dendritic sheath, are present in the lymph cavity of the sensilla chaetica. Long, medium, and short sensilla chaetica contain five, four and three dendrites, respectively. In contrast, no dendritic structure was observed in the cavity of the hairs. By immunocytochemistry experiments only odorant-binding protein 2 from L. migratoria (LmigOBP2) and chemosensory protein class I from the desert locust, Schistocerca gregaria Forsskål (SgreCSPI) strongly stained the outer lymph of sensilla chaetica of the cerci. The other two types of hairs were never labeled. The results indicate that the cerci might be involved in contact chemoreception processes

Quantitative characterization of Zr60700 alloy plate rolling in different directions based on electron back-scatter diffraction

Fully annealed Zr60700 alloy plates with a thickness of 15 mm were rolled at 15%, 30%, 45%, 60%, and 75% thickness reductions, and the rolling paths were unidirectional rolling (UR) and cross-rolling (CR), respectively. Electron back-scatter diffraction (EBSD) experiments revealed that the texture developed gradually towards the basal texture with increased thickness reduction. Severe orientation hardening enhanced the deformation resistance of the CR sample, resulting in a higher grain size than the UR sample. The plastic deformation during the rolling process was dominated by dislocation slip. The results of the prediction based on the Schmid factor (SF) and the calculation based on the in-grain misorientation axis (IGMA) showed that the prismatic slip was the primary deformation mode in the early stages of the UR and CR processes. With the increase in thickness reduction, the basal and pyramidal slip played an increasingly important role. Compared with the UR process, the CR process was more accessible to activate the basal slip and more conducive to forming the basal texture. Mechanical tests showed that the CR samples exhibited higher isotropy than the as-received and UR samples