Study of ovarian fluid biochemical parameters and its influence on spermatozoa motility of the Ship (Acipenser nudiventris Lovetzky, 1828) in the south-eastern Caspian Sea

Biochemical aspects of ovarian fluid were investigated in 10 specimens of the Ship (Acipenser nudiventris) by assessment of ionic organic composition and their relationships with osmolality. Also spermatozoa motility of the ship sturgeon was investigated in different percentage (5, 10, 25, 50 and 100%) of ovarian fluid (pooled 6 samples). Ovarian fluid contained 104.78±32.12mmol/1 Nat, 3.43±1.08mmol/ 1(7, 3.26±0.87mg/d1 Ca2+, 7.32±1.06rnEcil1 Mg^2+, 0.606±0.207mg/l protein, 24.88±13.02mg/l cholesterol and 60.35±14.49m/1 glucose. The pH of ovarian fluid ranged from 7.29 to 8.10 and osmolality ranged from 185 to 212msmol/Kg. There was also significant positive correlation between Nat concentration and osmolality (r = 0.835, P<0.01). When ship sturgeon semen diluted with 50 and 100% ovarian fluid, the spermatozoa remained immotile. The total duration of motility and percentage of motile spermatozoa was greatly reduced when semen was diluted in ovarian fluid higher than 5%. We found that the spermatozoa of ship sturgeon are immotile in the ovarian fluid because of ovarian fluid composition such as high concentration of K and osmotic pressure

The Effect of Extenders and Different Concentrations of Dimethyl Sulphoxide on Goldfish (Carassius auratus gibelio) Sperm

Abstract: Semen samples collected from eight males of Goldfish (Carassius auratus gibelio) were cryopreserved using four extenders (Goldfish sperm extenders; GFSE 1-GFSE 4) supplemented with Dimethyl Sulphoxide (DMSO) at concentration of 5%, 10% and 20%. Semen was diluted with ratio of 1:1 with extenders and frozen in liquid nitrogen vapor. Frozen sperms after 2 and 5 days were excluded from freezing. Experiment showed the highest motility duration and the most motility percentage of post-thawed sperms after 2 days was observed from sperm frozen at extender GFSE 2 with the concentration of DMSO 20%(49±10.53 s and 20.33±3.51%; P&lt;0.05), as well as the upmost mobility and the motility duration of post-thawed sperms after 5 days was observed from sperm frozen at extender GFSE 2 with the concentration of DMSO 10% (23.33±10.06s and 14.33±2.51%; P&lt;0.05)

Efficiency of 2-phenoxyethanol and Clove Oil for Reducing Handling Stress in Reared Meagre, Argyrosomus regius (Pisces: Sciaenidae)

The effect of two anesthetics, 2-phenoxyethanol (2-PE) and clove oil (COil) were studied in meagre, Argyrosomus regius. This study aimed to determine the adequate dose of anesthesia for different aquaculture procedures, to assess a sedative or stressor effect of low anesthetic concentrations, and to assess the effect of low anesthetic doses at high fish densities for longer periods of time on fish survival and behavior. Anesthetics were tested at different concentrations: 2-PE at 100, 250, 400, 550, and 700mg/L; COil at 25, 40, 55, 70, and 85mg/L. Meagre became anesthetized within 10min when using concentrations above 250mg/L for 2-PE and above 40mg/L for COil. Deep anesthesia was achieved at 700 and 85mg/L, for 2-PE and COil, respectively. The fish did not react when blood was collected at these concentrations. Plasma cortisol and glucose levels were similar between sedated meagre with both 2-PE-100mg/L and COil-10mg/L, and the control (not sedated; P>0.05). This suggests that low concentrations of these anesthetics induce similar stress response as handling without anesthesia during routine activities. No mortality was registered when meagre was maintained at high densities with low concentrations of each anesthetic for 2h

Motility of sea urchin Paracentrotus lividus

The characterization of sperm motility patterns, particularly post-activation changes, is the first step in setting up species-specific protocols involving gamete management and embryo production, for both aquaculture and laboratory research purposes. This study is aimed at the characterization of the sperm motility pattern of the purple sea urchin Paracentrotus lividus. Semen samples were individually diluted in artificial sea water for sperm motility activation. They were then incubated at 18°C for up to 24 hr. Motility was evaluated on dilution, and 1 hr, 3 hr and 24 hr after activation, by computerized analyser. The semen fertilization capacity was also evaluated. Under our experimental conditions (dilution 1:1,000 in artificial sea water plus 0.05% BSA, 18°C, in the dark), P. lividus semen remained viable for up to 24 hr, as the total motile sperm and the fertilization percentages did not change significantly during the incubation time. In contrast, the mean curvilinear velocity and the subpopulation of rapid sperm (those having a curvilinear velocity > 100 µm/s) slightly but significantly decreased after 3 hr, thereafter remaining unchanged for up to 24 hr after activation. In conclusion, our results show that diluted P. lividus semen can be used for a longer period than that of most fish species, with no need for motility inhibition procedures, supporting its wider use in laboratory research. In addition, the development of artificial fertilization protocols for aquaculture production is simplified by long-lasting sperm motility