Antimicrobial Activity Of New Synthetic Derivative Of Sesamol And Sesamum Indicum Seeds Extract Against Meningitis Causing Bacteria

The facts over S. aureus and E. coli to cause meningitis, and the antimicrobial potential of Sesame seeds and Sesamol were motivation for present study to compare the antibacterial potential of new synthetic derivativeof Sesamol (SDS) and Sesamum indicum seeds extract against meningitis causing bacteria (MCB). Present study involved synthesis of SDS and preparation of sesame seeds extract. The SDS was characterized using ATR-IR, 1H-NMR and Mass spectrometric data. Both SDS and sesame extract were tested for the inhibitory potential against MCB, namely: S. aureus and E. coli. Among both, the SDS exhibited higher inhibitory potential when compared with sesame extract. Based on the results present study concludes that SDS possess high inhibition potential against MCB and recommends that SDSmust be further evaluated for its clinical significance

Response Of New Sesamol Analogue And Sesamum Indicum Seeds Extract Against Meningitis Triggering Pathogens

A certain type of bacteria can cause an upper respiratory tract infection and then travel through the bloodstream to brain and can result in meningitis. Evidence over S. aureus and E. coli to trigger meningitis, antimicrobial potential of Sesamum indicumplant and sesamol intended present study to compare the antimicrobial response of new sesamol analogue (NSA) and Sesamum indicum seeds extract against meningitis triggering pathogens (MTP). Present study involved synthesis of NSA and preparation of sesame seeds extract (SSE). The NSA was characterized using ATR-IR, 1H-NMR and Mass spectrometric data. Both NSA and SSE were tested for antimicrobial potential against MTP, namely: S. aureus and E. coli. Among both, the NSA exhibited much higher antimicrobial potential when compared with SSE. Based on the resultant data present study concludes that NSA possess high inhibition potential against MTP and recommends that NSA should be further evaluated to support its clinical importance

Phylogeny in Aid of the Present and Novel Microbial Lineages: Diversity in Bacillus

Bacillus represents microbes of high economic, medical and biodefense importance. Bacillus strain identification based on 16S rRNA sequence analyses is invariably limited to species level. Secondly, certain discrepancies exist in the segregation of Bacillus subtilis strains. In the RDP/NCBI databases, out of a total of 2611 individual 16S rDNA sequences belonging to the 175 different species of the genus Bacillus, only 1586 have been identified up to species level. 16S rRNA sequences of Bacillus anthracis (153 strains), B. cereus (211 strains), B. thuringiensis (108 strains), B. subtilis (271 strains), B. licheniformis (131 strains), B. pumilus (83 strains), B. megaterium (47 strains), B. sphaericus (42 strains), B. clausii (39 strains) and B. halodurans (36 strains) were considered for generating species-specific framework and probes as tools for their rapid identification. Phylogenetic segregation of 1121, 16S rDNA sequences of 10 different Bacillus species in to 89 clusters enabled us to develop a phylogenetic frame work of 34 representative sequences. Using this phylogenetic framework, 305 out of 1025, 16S rDNA sequences presently classified as Bacillus sp. could be identified up to species level. This identification was supported by 20 to 30 nucleotides long signature sequences and in silico restriction enzyme analysis specific to the 10 Bacillus species. This integrated approach resulted in identifying around 30% of Bacillus sp. up to species level and revealed that B. subtilis strains can be segregated into two phylogenetically distinct groups, such that one of them may be renamed

Bioremediation of Mercury through Encapsulation of the Clone Carrying meroperon

Mercury (Hg) being one the most toxic element on the earth and its capability to result in neurotoxic disorder has become a serious problem for human health and environmental issues. Methyl mercury is the organic form of the mercury which easily accumulates within the aquatic animals and led to biomagnification in other higher organisms. Hg in the environment is resulted from unregulated discharge of mercury within the environment via numerous industries. Hence, it is crucial to bio-convert the toxic form of mercury to non-toxic form with the useful resource of microbes. One of the pronounced site of mercury contamination reported in India (Panipat) containing 147ppm Hg content, was selected to construct metagenomic library of mer operon using E.coli as host. The clone showed maximum tolerance towards mercury (90ppm) accompanied through effective volatilization (91.89% to 41.23%) for Hg (10-90ppm). The clone was able to efficiently bio-convert Hg in actual contaminated site as well. It was also encapsulated in sodium alginate beads and polyacrylamide gel in order to test its reusability for conversion of Hg

Bioconversion of Hg and Molecular Characterization of merA and merB Gene in a Clone Constructed by Culture Independent Technique

The anthropogenic use of mercury (Hg) has led to the wide outlook to the problem and health concerns caused by it. Metagenomic approach has opened the galore to the ease of access to the gene pool. The permissible limit of Hg is 0.001ppm but there are some sites in India having very high concentration of Hg (Ulhas estuary, Mumbai, having 107 ppm Hg). Library of clones containing mer operon, was constructed using culture independent technique. Out of 150 clones studied, UC07 has shown growth at 70ppm Hg. It has also shown antibiotic resistance towards vancomycin (30 mcg), Kanamycin (30 mcg) and Norfllaxin (10 mcg). Clone has the capability to volatilize 97.94% of 5ppm Hg which gradually decreased to 47% with the increase in Hg to 70ppm. Validation study using actual site (Ulhas soil) showed the effective conversion of Hg up to 99.9% by UC07. The clone contains both merA and merB genes, so it can be effectively utilized for organic and inorganic contaminated site. This is the first study of validation of clone using Hg contaminated site. The results are quite promising which shows that UC07 can be utilized effectively for bioconversion of both organic as well as inorganic form of Hg contamination