STABILITY INDICATING UPLC METHOD FOR QUANTIFICATION OF TOLPERISONE HCL AND PARACETAMOL FROM MUSCLE RELAXANT COMBINATION TABLET

Objective: In the present work, rapid and sensitive isocratic RP-UPLC method was established and comprehensive validation study for the estimation of Tolperisone HCl and Paracetamol was carried out according to international conference on harmonization (ICH) guidelines.Methods: Simultaneous estimation was chromatographed using 0.1% o-phosphoric acid in water and acetonitrile (70: 30 v/v) as a mobile phase at a flow rate of 0.20 ml/min with 35 ºC column temperature. Chromatographic separation accomplished isocratically on Acquity UPLC BEH C18 (50 mm ×2.1 mm, particle size 1.7 µm) and detection utilizing photodiode array detector at 254 nm. Injection volume was 2.0 µl.Results: The calibration curve was linear over the wide concentration range of 6.0µg/ml to 54.0µg/ml and 20.0µg/ml to 180.0µg/ml for Tolperisone HCl and Paracetamol, respectively. The retention time of Tolperisone HCl and Paracetamol was 1.396 and 2.625 min, respectively and the total analysis time was 5.0 min. Based on the results, the validated method was effectively applied for the estimation of Tolperisone HCl and Paracetamol in combined dosage form and in single pharmaceutical formulations with a new generation instrument, ultra performance liquid chromatography (UPLC). Moreover, the method helps to get better quality control and to pledge therapeutic efficacy.Conclusion: The method is simple, less time consuming and comparatively cost effective than existing methods.Â

Synthesis and crystal structure of a novel substituted 1,4-dihydropyridine

The title compound, C15H16N2O3, was synthesized, characterized spectroscopically, and finally confirmed by X-ray diffraction studies. The compound crystallizes in the monoclinic space group P21/n with cell parameters a = 10.314(9) Å, b = 17.976(15) Å, c = 12.762(11) Å, β = 113.331(3)°, Z = 4, and V = 2173(3) Å3. The dihydropyridine ring in the structure is in a flattened-boat conformation. The 2-nitrophenyl ring is orthogonal to the 1,4-dihydropyridine ring. The structure exhibits an intermolecular hydrogen bond of the type C–H…O

Novel dihydropyrimidinone derivatives as potential P-glycoprotein modulators

P-glycoprotein (Pgp), an ATP binding cassette (ABC) transporter, is an ATP-dependent efflux pump responsible for cancer multidrug resistance. As part of efforts to identify human Pgp (hPgp) inhibitors, we prepared a series of novel triazole-conjugated dihydropyrimidinones using a synthetic approach that is well suited for obtaining compound libraries. Several of these dihydropyrimidinone derivatives modulate human P-glycoprotein (hPgp) activity with low micromolar EC50 values. Molecular docking studies suggest that these compounds bind to the M-site of the transporter

3,5-Diacetyl-1,4-dihydropyridines: Synthesis and MDR Reversal in Tumor Cells

The International Institute of Anticancer Researc

Synthesis of monomethylated dimeric benzopyrans as HIV-1 and HIV-2 inhibitors: Part I

2197-2199Mono methylated dimeric coumarins have been synthesized by condensation of 6- or 8-methyl-4-hydroxycoumarins with different aromatic aldehydes. Twenty three compounds or the two series have been screened for anti -HIV activity against HIV-1 and HIV-2 strains

Synthesis and antimicrobial screening of some arylaminocoumarins

1502-1507The phenolic hydroxyl group of 4-hydroxycoumarins is replaced by different amines in a single step method to get new substituted 4-amino derivatives