Influence of the Different Carbon and Nitrogen Sources on the Production of Biodiesel by Oleaginous Fungi Aspergillus terreus, Aspergillus fumigatus

في الدراسة الحاليه  تمت المقارنه بين تاثير مصادر كربونيه ونتروجينيه مختلفه على النمو ومحتوى الدهون في عزلتين من الفطريات المنتجه للدهون    Aspergillus  terreus و Aspergillus fumigatus  . زرعت العزلتين الفطريه على اوساط زرعيه حاويه على مصادر كربونيه مختلفه السكريات الاحاديه والثنائية والمتعدده  ومصادر نتروجينيه متضمنه الببتون وخلاصة الخميرة  وكانت الاوساط الحاويه على السكروز / خلاصة الخميره  او السكروز / خلاصة الخميره اكثر تاثيرا على انتاجيه الدهون للعزلتين الفطريه خلال فترة حضانه اسبوعين حيث بلغت   اعلى قيمة للكتلة الحيويه المتمثلة بالوزن الجاف (19.6 , 18.8)   (25.8 , 30.5 ) ,   غم / لتر ونسبة الدهون  (1, 0.97 )g/L  , (0.65 , 0.65) غم / لتر لكلا العزلنين الفطريه Aspergillus  terreus   و   Aspergillus fumigatus  على الترتيب ثم تبعتها اوساط الغنيه  بالمالتوز/ خلاصة الخميرة واخيرا النشا  / خلاصة الخميرة . وفيما يخص تحليل الحوامض الدهنيه بواسطه جهاز كروموتوكرافيا الغاز Gas Chromotography   والمنتجه من قبل كلا  العزلتين الفطريتين لوحظ وجود انواع مختلفه تضمنت   Palmitic   Oleic acids , stearic acid   و linoleic acid   اي المشبعه والغير مشبعه. اظهرت النتائج تفاوت في تركيب الحوامض الدهنيه يعتمد على نوع مصادر الكربون والنتروجين , وجود الحوامض الدهنيه المشبعه الاحاديه والغير مشبعه في كلا الفطرين  A. terreus و  A.fumigatusيثبت كونهما مرشحان جيدين في انتاج الديزل الحيوي.In the present study, the growth and total lipid contents of two oleaginous fungal isolates Aspergillus terreus, Aspergillus fumigatus were compared in different nitrogen and organic carbon sources.  Artificially the fungi were cultured on media consisting of various mono- or di- or polysaccharides and  peptone or yeast extract as elementary sources for carbon and nitrogen, respectively. Media containing sucrose /yeast extract or glucose/ yeast extract were the most effective for lipid production from fungal, during  two weeks  incubation period, the highest biomass of dry weight was (19.6 , 18.8) g / L , (25.8 , 30.5) g /L  and  lipid yield (1, 0.97 )g/L, (0.65, 0.65) g/ L for two isolates Aspergillus terreus and Aspergillus fumigatus  respectively then followed maltose/ yeast extract then  starch / yeast extract .Analysis of fatty acid produced by two fungal isolates  using Gas Chromatography  showed the existence of a wide range of fatty acids, these fatty acids were together saturated and unsaturated. The results revealed that the unevenness in the fatty acids composition mainly depends on the type of carbon and nitrogen sources. The existence of saturated and mono saturated fatty acids in A.fumigatus, A. terreus prove that they are  good candidate for biodiesel production. The analysis of fatty acid from the fungal growth showed the existence of a wide range of fatty acids   Palmitic ,Oleic acids , stearic acid and linoleic acid     together saturated and unsaturated. The results revealed that the unevenness in the fatty acid composition mainly depends on the type  of carbon  and nitrogen sources. The presence of saturated and mono saturated fatty acid in  A.fumigatus , A. terreus prove that it is a good candidate for biodiesel production

KCNT1- related epilepsy: An international multicenter cohort of 27 pediatric cases

ObjectiveThrough international collaboration, we evaluated the phenotypic aspects of a multiethnic cohort of KCNT1- related epilepsy and explored genotype- phenotype correlations associated with frequently encountered variants.MethodsA cross- sectional analysis of children harboring pathogenic or likely pathogenic KCNT1 variants was completed. Children with one of the two more common recurrent KCNT1 variants were compared with the rest of the cohort for the presence of particular characteristics.ResultsTwenty- seven children (15 males, mean age = 40.8 months) were included. Seizure onset ranged from 1 day to 6 months, and half (48.1%) exhibited developmental plateauing upon onset. Two- thirds had epilepsy of infancy with migrating focal seizures (EIMFS), and focal tonic seizures were common (48.1%). The most frequent recurrent KCNT1 variants were c.2800G>A; p.Ala934Thr (n = 5) and c.862G>A; p.Gly288Ser (n = 4). De novo variants were found in 96% of tested parents (23/24). Sixty percent had abnormal magnetic resonance imaging (MRI) findings. Delayed myelination, thin corpus callosum, and brain atrophy were the most common. One child had gray- white matter interface indistinctness, suggesting a malformation of cortical development. Several antiepileptic drugs (mean = 7.4/patient) were tried, with no consistent response to any one agent. Eleven tried quinidine; 45% had marked (>50% seizure reduction) or some improvement (25%- 50% seizure reduction). Seven used cannabidiol; 71% experienced marked or some improvement. Fourteen tried diet therapies; 57% had marked or some improvement. When comparing the recurrent variants to the rest of the cohort with respect to developmental trajectory, presence of EIMFS, >500 seizures/mo, abnormal MRI, and treatment response, there were no statistically significant differences. Four patients died (15%), none of sudden unexpected death in epilepsy.SignificanceOur cohort reinforces common aspects of this highly pleiotropic entity. EIMFS manifesting with refractory tonic seizures was the most common. Cannabidiol, diet therapy, and quinidine seem to offer the best chances of seizure reduction, although evidence- based practice is still unavailable.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154940/1/epi16480_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154940/2/epi16480.pd

KCNT1‐related epilepsy: An international multicenter cohort of 27 pediatric cases

ObjectiveThrough international collaboration, we evaluated the phenotypic aspects of a multiethnic cohort of KCNT1- related epilepsy and explored genotype- phenotype correlations associated with frequently encountered variants.MethodsA cross- sectional analysis of children harboring pathogenic or likely pathogenic KCNT1 variants was completed. Children with one of the two more common recurrent KCNT1 variants were compared with the rest of the cohort for the presence of particular characteristics.ResultsTwenty- seven children (15 males, mean age = 40.8 months) were included. Seizure onset ranged from 1 day to 6 months, and half (48.1%) exhibited developmental plateauing upon onset. Two- thirds had epilepsy of infancy with migrating focal seizures (EIMFS), and focal tonic seizures were common (48.1%). The most frequent recurrent KCNT1 variants were c.2800G>A; p.Ala934Thr (n = 5) and c.862G>A; p.Gly288Ser (n = 4). De novo variants were found in 96% of tested parents (23/24). Sixty percent had abnormal magnetic resonance imaging (MRI) findings. Delayed myelination, thin corpus callosum, and brain atrophy were the most common. One child had gray- white matter interface indistinctness, suggesting a malformation of cortical development. Several antiepileptic drugs (mean = 7.4/patient) were tried, with no consistent response to any one agent. Eleven tried quinidine; 45% had marked (>50% seizure reduction) or some improvement (25%- 50% seizure reduction). Seven used cannabidiol; 71% experienced marked or some improvement. Fourteen tried diet therapies; 57% had marked or some improvement. When comparing the recurrent variants to the rest of the cohort with respect to developmental trajectory, presence of EIMFS, >500 seizures/mo, abnormal MRI, and treatment response, there were no statistically significant differences. Four patients died (15%), none of sudden unexpected death in epilepsy.SignificanceOur cohort reinforces common aspects of this highly pleiotropic entity. EIMFS manifesting with refractory tonic seizures was the most common. Cannabidiol, diet therapy, and quinidine seem to offer the best chances of seizure reduction, although evidence- based practice is still unavailable.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154940/1/epi16480_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154940/2/epi16480.pd