Assessment of DFG-S19 method for the determination of common endocrine disruptor pesticides in wine samples with an estimation of the uncertainty of the analytical results

WOS: 000314193800009PubMed ID: 23265455A gas chromatographic method for the determination of endocrine disruptor pesticides (Chlorpyrifos, Penconazole, Procymidone, Iprodione, Bromopropylate and Lambda-Cyhalothrin) in wine samples is described. A general DFG-S19 method for residual pesticide determination in all kind of food stuff was investigated to simplify and adopt for wine samples in this work. Alternative sample preparation routes were elucidated and compared according to their recovery values. Four different separation techniques were tested and the method employing florosil column after a LLE procedure was applied for wine samples with satisfactory recovery ratios (72-97%). The pesticides were extracted from the sample by cyclohexane-ethyl acetate mixture (1:1 v/v) and cleaned up by florosil column. The regression coefficients were at least 0.99 and relative standard deviations were no higher than 16%. Detection limits were in the range of 0.6-2.9 ng/mL and the relative expanded measurement uncertainties were calculated in the 7-22% range. (C) 2012 Elsevier Ltd. All rights reserved.Ege UniversityEge University [2005/Fen/017]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108T660]The authors thank to the Ege University for financial support (Project 2005/Fen/017) and TUBITAK ((Project 108T660)