Evaluation of 99mTc-Cefuroxime axetil for imaging of inflammation

20th Annual Congress of the European-Association-of-Nuclear-Medicine -- 2007 -- Copenhagen, DENMARKWOS: 000253283900669European Assoc Nucl Me

Evaluation of Tc-99m-Cefuroxime axetil for imaging of inflammation

Localizing and distinguishing the "infection" in body sites are very important and life saving processes. Scintigraphic detections may help to determine the sites of inflammation and infection. At this point, nuclear medical imaging may proceed one step further and be helpful to localize and distinguish the inflammation. The radiolabeled antibiotic Tc-99m-Cefuroxime axetil was assessed as an infection imaging agent in a rat model. In this study, Tc-99m-Cefuroxime axetil was examined in localizing the normal, sterile inflamed, and septic inflamed rat muscle tissues, and also in distinguishing each of them. The biodistribution data show that Tc-99m labeled Cefuroxime axetil was retained in infectious areas. The retention was better in septic inflamed (S. aureus) area than sterile inflamed area. The clearance of the labeled antibiotic from other tissues is rapid on the contrary to its clearance from the septic area. Target/non-target ratio shows a good value of 2.5 at 4-hour post injection when the activity of the other organs is cleared by urinary excretion

Imaging of bacterial infection with Tc-99m-labeled HBD-1

The aim of this study was to evaluate Tc-99m labeled human beta-defensin-1 (HBD-1) for discrimination between bacterial infection and sterile inflammation. For this purpose, HBD-1 was radiolabeled with 99mTc and its in vivo distribution was evaluated in inflamed rats with Staphylococcus aureus (S. aureus) and sterile inflamed rats with turpentine oil. After injection into inflamed and sterile inflamed rats, Tc-99m-HBD-1 was rapidly removed from the circulation via the kidneys. Binding of Tc-99m-HBD-1 to inflamed muscle (T/NT 20 at 120 min) was two times higher than binding to sterile inflamed muscle (T/NT = 10 at 120 min) of rats. It was demonstrated that Tc-99m-HBD-1 can be used to detect S. aureus inflammation in rats. However, the radiolabeled antimicrobial peptide showed only poor uptake in sterile inflammation with turpentine oil in rats. As a result, Tc-99m-HBD-1 can be useful for detection of bacterial inflammation

Radiolabeling of codeine with I-131 and its biodistribution in rats

2nd International Nuclear Chemistry Congress (2nd-INCC) -- APR 13-17, 2008 -- Cancun, MEXICOWOS: 000265714000028Codeine which was extracted from dry capsules of the opium poppy (Papaver somniferum) was purified by HPLC (High Performance Liquid Chromatography) and characterized by NMR (Nuclear Magnetic Resonance) and IR (Infrared) spectroscopy techniques. The purified compound was labeled with I-131 and biodistribution studies were performed in rats. Radioiodinated codeine distributed uniformly in the cerebellum, m.pons, striatum and hypothalamus while the other branch of brain and Stomach, urinary bladder, and small intestine uptakes were significantly higher than other tissues