A Brief Overview on Ferrite (Fe3O4) Based Polymeric Nanocomposites: Recent Developments and Challenges

In this article, we have mainly discussed about ferrite (Fe3O4) and its polymer based nanocomposites. Ferrite particles have become an important research material because of their vast applications in the field of biotechnology, magnetic resonance imaging (MRI), and data storage. It has been observed that ferrite Fe3O4 particles show best performance for size less than 10-30 nm. This happens due to the super paramagnetic nature of such particles. In super paramagnetic range these particles exhibit zero remanence or coercivity. Therefore, various properties of ferrite (Fe3O4) nanoparticles and its polymer nanocomposites are very much dependent on the size, and distribution of the particles in the polymeric matrix. Moreover, it has been also observed that the shape of the nanocrystals plays important role in the determination of their fundamental properties. These particles show instability over longer times due to the formation of agglomerates generated by high surface energies. Therefore, protection strategies such as grafting and coatings with silica/carbon or polymers have been developed to stabilize them chemically. Recently, silylation technique is mainly used for the modification of nanoparticles. Experimentally, it has been observed that nanocomposites composed of polymer matrices and ferrite showed substantial improvements in stiffness, fracture toughness, sensing ability (magnetic as well as electric), impact energy absorption, and electro-catalytic activities to bio-species

Clinical Significance of MUC13 in Pancreatic Ductal Adenocarcinoma

Background—Poor prognosis of pancreatic cancer (PanCa) is associated with lack of an effective early diagnostic biomarker. This study elucidates significance of MUC13, as a diagnostic/prognostic marker of PanCa. Methods—MUC13 was assessed in tissues using our in-house generated anti-MUC13 mouse monoclonal antibody and analyzed for clinical correlation by immunohistochemistry, immunoblotting, RT-PCR, computational and submicron scale mass-density fluctuation analyses, ROC and Kaplan Meir curve analyses. Results—MUC13 expression was detected in 100% pancreatic intraepithelial neoplasia (PanIN) lesions (Mean composite score: MCS=5.8; AUC \u3e0.8, P\u3c0.0001), 94.6% of pancreatic ductal adenocarcinoma (PDAC) samples (MCS=9.7, P\u3c0.0001) as compared to low expression in tumor adjacent tissues (MCS=4, P\u3c0.001) along with faint or no expression in normal pancreatic tissues (MCS=0.8; AUC \u3e0.8; P\u3c0.0001). Nuclear MUC13 expression positively correlated with nodal metastasis (P\u3c0.05), invasion of cancer to peripheral tissues (P\u3c0.5) and poor patient survival (P\u3c0.05; prognostic AUC=0.9). Submicron scale mass density and artificial intelligence based algorithm analyses also elucidated association of MUC13 with greater morphological disorder (P\u3c0.001) and nuclear MUC13 as strong predictor for cancer aggressiveness and poor patient survival. Conclusion—This study provides significant information regarding MUC13 expression/ subcellular localization in PanCa samples and supporting the use anti-MUC13 MAb for the development of PanCa diagnostic/prognostic test

Cold atmospheric plasma induces ATP-dependent endocytosis of nanoparticles and synergistic U373MG cancer cell death

Gold nanoparticles (AuNP) have potential as both diagnostic and therapeutic vehicles. However, selective targeting and uptake in cancer cells remains challenging. Cold atmospheric plasma (CAP) can be combined with AuNP to achieve synergistic anti-cancer cytotoxicity. To explore synergistic mechanisms, we demonstrate both rate of AuNP uptake and total amount accumulated in U373MG Glioblastoma multiforme (GBM) cells are significantly increased when exposed to 75 kV CAP generated by dielectric barrier discharge. No significant changes in the physical parameters of AuNP were caused by CAP but active transport mechanisms were stimulated in cells. Unlike many other biological effects of CAP, long-lived reactive species were not involved, and plasma-activated liquids did not replicate the effect. Chemical effects induced by direct and indirect exposure to CAP appears the dominant mediator of enhanced uptake. Transient physical alterations of membrane integrity played a minor role. 3D-reconstruction of deconvoluted confocal images confirmed AuNP accumulation in lysosomes and other acidic vesicles, which will be useful for future drug delivery and diagnostic strategies. Toxicity of AuNP significantly increased by 25-fold when combined with CAP. Our data indicate that direct exposure to CAP activates AuNP-dependent cytotoxicity by increasing AuNP endocytosis and trafficking to lysosomes in U373MG cells

Cisplatin-tethered gold nanospheres for multimodal chemo-radiotherapy of glioblastoma

Glioblastoma multiforme (GBM) remains the most aggressive and challenging brain tumour to treat. We report the first successful chemo-radiotherapy on patient derived treatment resistant GBM cells using a cisplatin-tethered gold nanosphere. After intracellular uptake, the nanosphere effects DNA damage which initiates caspase-mediated apoptosis in those cells. In the presence of radiation, both gold and platinum of cisplatin, serve as high atomic number radiosensitizers leading to the emission of ionizing photoelectrons and Auger electrons. This resulted in enhanced synergy between cisplatin and radiotherapy mediated cytotoxicity, and photo/Auger electron mediated radiosensitisation leading to complete ablation of the tumour cells in an in vitro model system. This study demonstrates the potential of designed nanoparticles to target aggressive cancers in the patient derived cell lines providing a platform to move towards treatment strategies. © the Partner Organisations 2014