5 research outputs found
The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution
BACKGROUND:Giardia lamblia trophozoites colonize the intestines of susceptible mammals and cause diarrhea, which can be prolonged despite an intestinal immune response. The variable expression of the variant-specific surface protein (VSP) genes may contribute to this prolonged infection. Only one is expressed at a time, and switching expression from one gene to another occurs by an epigenetic mechanism.RESULTS:The WB Giardia isolate has been sequenced at 10x coverage and assembled into 306 contigs as large as 870 kb in size. We have used this assembly to evaluate the genomic organization and evolution of the vsp repertoire. We have identified 228 complete and 75 partial vsp gene sequences for an estimated repertoire of 270 to 303, making up about 4% of the genome. The vsp gene diversity includes 30 genes containing tandem repeats, and 14 vsp pairs of identical genes present in either head to head or tail to tail configurations (designated as inverted pairs), where the two genes are separated by 2 to 4 kb of non-coding DNA. Interestingly, over half the total vsp repertoire is present in the form of linear gene arrays that can contain up to 10 vsp gene members. Lastly, evidence for recombination within and across minor clades of vsp genes is provided.CONCLUSIONS:The data we present here is the first comprehensive analysis of the vsp gene family from the Genotype A1 WB isolate with an emphasis on vsp characterization, function, evolution and contributions to pathogenesis of this important pathogen.This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at [email protected]
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Protein coding transcription and control of vsp gene expression in the protozoan parasite Giardia lamblia
Giardia lamblia is an early branching eukaryote and although distinctly eukaryotic in its cell and molecular biology, transcription in G. lamblia demonstrates important differences from these processes in higher eukaryotes. α-amanitin is a relatively selective inhibitor of eukaryotic RNA Polymerase II (RNAP II), and is commonly used to study RNAP II transcription. Therefore, we measured the sensitivity of G. lamblia RNAP II transcription to α-amanitin and found that unlike most other eukaryotes, RNAP II transcription in Giardia is resistant to 1 mg/ml amanitin. To better understand transcription in G. lamblia, we identified 10 out of the 12 known eukaryotic rph genes, including all ten that are required for viability in Saccharomyces cerevisiae. The amanitin motif (amanitin binding site) of Rpbl from G. lamblia differs from other eukaryotes at six highly conserved sites in which substitutions have been associated with amanitin resistance in other organisms. These observations of amanitin resistance provide a molecular framework for the development of novel drugs with selective activity against G. lamblia. Giardia trophozoites exhibit antigenic variation of a surface protein encoded by a family of genes known as the vsp genes. A single trophozoite expresses only one vsp at a time and it has been previously determined that steady state mRNA of only the expressed vsp is detectable in Northern blots. Our nuclear run-on assays indicated transcription of only the expressed vsp genes, suggesting that control is primarily at the level of transcription rather than post-transcription. In order to better understand vsp gene control mechanisms, we used a luciferase reporter to determine the vsp core promoter, which is present within 100 bp upstream of the ORF in the case of vspCS and vspA6. The fact that the vsp promoter is able to drive expression irrespective of the antigenic variant type indicates that control requires a chromosomal context as do most epigenetic mechanisms of control. Based on the existing data, we provide a privileged site model for the control of vsp gene expression, in which vsp transcription takes place in a set nuclear location which is occupied by a singe vsp locus at a time
The <it>Giardia lamblia vsp </it>gene repertoire: characteristics, genomic organization, and evolution
Abstract Background Giardia lamblia trophozoites colonize the intestines of susceptible mammals and cause diarrhea, which can be prolonged despite an intestinal immune response. The variable expression of the variant-specific surface protein (VSP) genes may contribute to this prolonged infection. Only one is expressed at a time, and switching expression from one gene to another occurs by an epigenetic mechanism. Results The WB Giardia isolate has been sequenced at 10× coverage and assembled into 306 contigs as large as 870 kb in size. We have used this assembly to evaluate the genomic organization and evolution of the vsp repertoire. We have identified 228 complete and 75 partial vsp gene sequences for an estimated repertoire of 270 to 303, making up about 4% of the genome. The vsp gene diversity includes 30 genes containing tandem repeats, and 14 vsp pairs of identical genes present in either head to head or tail to tail configurations (designated as inverted pairs), where the two genes are separated by 2 to 4 kb of non-coding DNA. Interestingly, over half the total vsp repertoire is present in the form of linear gene arrays that can contain up to 10 vsp gene members. Lastly, evidence for recombination within and across minor clades of vsp genes is provided. Conclusions The data we present here is the first comprehensive analysis of the vsp gene family from the Genotype A1 WB isolate with an emphasis on vsp characterization, function, evolution and contributions to pathogenesis of this important pathogen.</p
Assessing the Efficiency of RNA Interference for Maize Functional Genomics
A large-scale functional genomics project was initiated to study the function of chromatin-related genes in maize (Zea mays). Transgenic lines containing short gene segments in inverted repeat orientation designed to reduce expression of target genes by RNA interference (RNAi) were isolated, propagated, and analyzed in a variety of assays. Analysis of the selectable marker expression over multiple generations revealed that most transgenes were transmitted faithfully, whereas some displayed reduced transmission or transgene silencing. A range of target-gene silencing efficiencies, from nondetectable silencing to nearly complete silencing, was revealed by semiquantitative reverse transcription-PCR analysis of transcript abundance for the target gene. In some cases, the RNAi construct was able to cause a reduction in the steady-state RNA levels of not only the target gene, but also another closely related gene. Correlation of silencing efficiency with expression level of the target gene and sequence features of the inverted repeat did not reveal any factors capable of predicting the silencing success of a particular RNAi-inducing construct. The frequencies of success of this large-scale project in maize, together with parameters for optimization at various steps, should serve as a useful framework for designing future RNAi-based functional genomics projects in crop plants