Tailored polities in the shadow of the state's hierarchy. The CLLD implementation and a future research agenda

The paper provides a theoretical contribution to the multi-level governance debate, discussing the role of the policy instruments in tailoring polities for local development strategies. To this purpose, it examines the Community-Led Local Development (CLLD), a policy tool of the EU Cohesion Policy 2014–2020, which has generated more than 3000 local initiatives across the EU. An institutionalist perspective enables a reflection on the multi-level normative dimensions of these local initiatives. A combination of the post-functionalist governance theory, the soft space debate, state-theory and strategic-relational approach provides an interpretative framework to be deployed for a dedicated research agenda. The interpretative challenge is about whether the CLLD enables spatial-temporal fixes in which a deliberative polity pursues a spatial imaginary for an ad-hoc territory. The consequent analytical dimensions can be found in (a) the relationship between attendant ad-hoc polity, policy agenda, territorial design and societal processes; and (b) the meta-governance dimensions that locate the bottom-up constituency of this institutional technology in the shadow of state’s hierarchy. An overview of the CLLD implementation across the EU provides evidence on the latter

1-N6-Etheno-ADP-ribosylation of elongation factor-2 by diphtheria toxin

AbstractDiphtheria toxin fragment A is able to inhibit protein synthesis in the eukaryotic cell by ADP-ribosylating the diphthamide residue of elongation factor-2 (EF-2) [(1980) J. Biol. Chem. 255, 10710-10720]. The reaction requires NAD as ADP-ribose donor. This work reports on the capacity of an NAD analog, the nicotinamide 1-N6-ethenoadenine dinucleotide (ϵNAD), to be a substrate of diphtheria toxin fragment A in the transferring reaction of the fluorescent moiety, the ϵADP-ribose, to the EF-2. As a consequence of the transfer of the ϵADP-ribosyl moiety to the EF-2, there is an increase in the emission intensity of the fluorophore and a blue shift in its emission maximum. The ϵADP-ribosylated EF-2, like ADP-ribosylated EF-2, retains the capacity to bind GTP and ribosome. The utility of introducing a fluorescent probe in a well defined point of the EF-2 molecule for conformational or binding studies is discussed

ESPON TOWN spin-off Denmark. Small and medium sized towns in Denmark

This study “ESPON TOWN spin-off Denmark” provides supporting knowledge and evidence on the state and development of SMSTs in Denmark by using detailed datasets and by conducting an in-depth analysis of the local context . Based on a quantitative data-driven analysis, it describes the main characteristics of SMSTs in Denmark, how they differ from each other and what role various aspects including tourism and services play. Furthermore, it presents a short update of SMST research as well as a recap of recent Danish policies related to SMSTs. Finally, it provides perspectives on Denmark’s SMSTs within a European policy context

Insulin-like growth factor-1 as predictive factor of difficult laryngoscopy in patients with GH-producing pituitary adenoma: A pilot study

Background: Growth hormone (GH)-producing pituitary tumors account for 10 to 15% of pituitary tumors. The hypersecretion of GH may induce changes in the airway anatomy through the activation of Insulin-like Growth factor 1(IGF-1) pathway. We sought investigate the role IGF-1 as a potential predictive factor of difficult laryngoscopy in patients with GH-producing pituitary adenoma. Methods: This study was a single center retrospective study. We included 33 patients undergoing transsphenoidal resection of GH-producing pituitary. We recorded demographic data, el-Ganzouri risk index (EGRI) and modified Look-Evaluate-Mallampati-Obstruction-Neck mobility (mLEMON) score, and pituitary hormone plasma levels. We performed ordinal logistic regression to analyze the relationship between IGF-1 and EGRI, mLEMON, and Cormack-Lehane Grade score and a multiple logistic regression to test the capability of EGRI, mLEMON and IGF-1 levels to predict Cormack-Lehane score. Receiver operating curve (ROC), area under the curve (AUC), and cut-off value of IGF-1 were calculated. Results: Only 14 (42.8%) and 12 (36.36%) patients showed predictive factors of difficult intubation according to EGRI and mLEMON score, respectively. IGF-1 significantly correlated with Cormack-Lehane (p = 0.005879) but not with mLEMON and EGRI (p = 0.3080 and 0.4146, respectively). In multiple regression model IGF-1 correlated only with Cormack-Lehane grade (p = 0.0089). Area under ROC was 0.8571 and cut-off value of IGF-1 was 186.15 ng/ml. Conclusion: Higher IGF-1 levels correlate with the probability of having a higher Cormack-Lehane score; classical bedside scores, such as mLEMON and EGRI, were not able to predict difficult laryngoscopy in our population

‘Left behind places’: a geographical etymology

‘Left behind places’ has become the leitmotif of geographical inequalities since the 2008 crisis. Yet, the term’s origins, definition and implications are poorly specified and risk obscuring the differentiated problems and pathways of different kinds of areas. This paper explicates the geographical etymology and spatial imaginary of ‘left behind places’. It argues that the appellation and its spatial expression have modified how geographical inequalities are understood and addressed by recovering a more relational understanding of multiple ‘left behind’ conditions, widening the analytical frame beyond only economic concerns, and opening up interpretations of the ‘development’ of ‘left behind places’ and their predicaments and prospects. While renewing interest in fundamental urban and regional concerns, what needs to endure from the ascendance of the ‘left behind places’ label is the terminology and spatial imaginary of reducing geographical inequalities and enhancing social and spatial justice

'Left behind places': a geographical etymology

'Left behind places' has become the leitmotif of geographical inequalities since the 2008 crisis. Yet, the term's origins, definition and implications are poorly specified and risk obscuring the differentiated problems and pathways of different kinds of areas. This paper explicates the geographical etymology and spatial imaginary of 'left behind places'. It argues that the appellation and its spatial expression have modified how geographical inequalities are understood and addressed by recovering a more relational understanding of multiple 'left behind' conditions, widening the analytical frame beyond only economic concerns, and opening up interpretations of the 'development' of 'left behind places' and their predicaments and prospects. While renewing interest in fundamental urban and regional concerns, what needs to endure from the ascendance of the 'left behind places' label is the terminology and spatial imaginary of reducing geographical inequalities and enhancing social and spatial justice

Perturbation of serine enantiomers homeostasis in the striatum of MPTP-lesioned monkeys and mice reflects the extent of dopaminergic midbrain degeneration

Loss of dopaminergic midbrain neurons perturbs L-serine and D-serine homeostasis in the post-mortem caudate putamen (CPu) of Parkinson's disease (PD) patients. However, it is unclear whether the severity of dopaminergic nigrostriatal degeneration plays a role in deregulating serine enantiomers' metabolism. Here, through high -performance liquid chromatography (HPLC), we measured the levels of these amino acids in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated monkeys and MPTP-plus-probenecid (MPTPp)-treated mice to determine whether and how dopaminergic midbrain degeneration affects the levels of serine enantiomers in various basal ganglia subregions. In addition, in the same brain regions, we measured the levels of key neuro-active amino acids modulating glutamatergic neurotransmission, including L-glutamate, glycine, L-aspartate, D- aspartate, and their precursors L-glutamine, L-asparagine. In monkeys, MPTP treatment produced severe denervation of nigrostriatal dopaminergic fibers (⁓75%) and increased the levels of serine enantiomers in the rostral putamen (rPut), but not in the subthalamic nucleus, and the lateral and medial portion of the globus pallidus. Moreover, this neurotoxin significantly reduced the protein expression of the astrocytic serine trans-porter ASCT1 and the glycolytic enzyme GAPDH in the rPut of monkeys. Conversely, concentrations of D-serine and L-serine, as well as ASCT1 and GAPDH expression were unaffected in the striatum of MPTPp-treated mice, which showed only mild dopaminergic degeneration (⁓30%). These findings unveil a link between the severity of dopaminergic nigrostriatal degeneration and striatal serine enantiomers concentration, ASCT1 and GAPDH expression. We hypothesize that the up-regulation of D-serine and L-serine levels occurs as a secondary response within a homeostatic loop to support the metabolic and neurotransmission demands imposed by the degener-ation of dopaminergic neurons