1,802 research outputs found
Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients
Introduction: There are limited findings available on KIT-negative GIST-like (KNGL) population. Also, KIT expression may be post-transcriptionally regulated by miRNA221 and miRNA222. Hence, the aim of this study is to characterize KNGL population, by differential gene expression, and to analyze miRNA221/222 expression and their prognostic value in KNGL patients.
Methods: KIT, PDGFRA, DOG1, IGF1R, MIR221 and MIR222 expression levels were determined by qRT-PCR. We also analyzed KIT and PDGFRA mutations, DOG1 expression, by immunohistochemistry, along with clinical and pathological data. Disease-free survival (DFS) and overall survival (OS) differences were calculated using Log-rank test.
Results: Hierarchical cluster analyses from gene expression data identified two groups: group I had KIT, DOG1 and PDGFRA overexpression and IGF1R underexpression and group II had overexpression of IGF1R and low expression of KIT, DOG1 and PDGFRA. Group II had a significant worse OS (p = 0.013) in all the series, and showed a tendency for worse OS (p = 0.11), when analyzed only the localized cases. MiRNA222 expression was significantly lower in a control subset of KIT-positive GIST (p < 0.001). OS was significantly worse in KNGL cases with higher expression of MIR221 (p = 0.028) or MIR222 (p = 0.014).
Conclusions: We identified two distinct KNGL subsets, with a different prognostic value. Increased levels of miRNA221/222, which are associated with worse OS, could explain the absence of KIT protein expression of most KNGL tumors
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care
Funding Information: The authors acknowledge funding through project TecniCov ( POCI-01-02B7-FEDER-069745 ), co-funded by FEDER through COMPETE2020 and Lisboa2020 and CY-Sensors ( POCI-01-0145-FEDER-032359 ) through Fundação para a Ciência e a Tecnologia (FCT) , Portugal. ARC acknowledge funding to National Foundation for Science and Technology , I.P., Portugal ( FCT ) through the PhD. Grant, reference SFRH/BD/130107/2017 . Publisher Copyright: © 2022 The AuthorsThis work presents an innovative ultra-sensitive biosensor having the Spike protein on carbon-based screen-printed electrodes (SPEs), for monitoring in point-of-care antibodies against SARS-CoV-2, a very important tool for epidemiological monitoring of COVID-19 infection and establishing vaccination schemes. In an innovative and simple approach, a highly conductive support is combined with the direct adsorption of Spike protein to enable an extensive antibody capture. The high conductivity was ensured by using carboxylated carbon nanotubes on the carbon electrode, by means of a simple and quick approach, which also increased the surface area. These were then modified with EDC/NHS chemistry to produce an amine layer and undergo Spike protein adsorption, to generate a stable layer capable of capturing the antibodies against SARS-CoV-2 in serum with great sensitivity. Electrochemical impedance spectroscopy was used to evaluate the analytical performance of this biosensor in serum. It displayed a linear response between 1.0 pg/mL and 10 ng/mL, with a detection limit of ∼0.7 pg/mL. The analysis of human positive sera containing antibody in a wide range of concentrations yielded accurate data, correlating well with the reference method. It also offered the unique ability of discriminating antibody concentrations in sera below 2.3 μg/mL, the lowest value detected by the commercial method. In addition, a proof-of-concept study was performed by labelling anti-IgG antibodies with quantum dots to explore a new electrochemical readout based on the signal generated upon binding to the anti-S protein antibodies recognised on the surface of the biosensor. Overall, the alternative serologic assay presented is a promising tool for assessing protective immunity to SARS-CoV-2 and a potential guide for revaccination.publishersversionpublishe
Substitute therapy of the sclera. Identification of the patologies and methodologies in the University Hospital of Granada (2014-2019)
Introducción: La esclera es un tejido avascular compuesto por tejido conectivo denso cuya función principal es proteger las estructuras intraoculares. Existen diversas patologías que ponen en riesgo la integridad de dicho tejido y, en consecuencia, pueden provocar terribles secuelas con un pronóstico nefasto.
El tratamiento sustitutivo forma parte de la terapia de dicha patología, siendo el injerto de esclerótica
cadáver una opción biocompatible y biomimética a valorar. El injerto de esclerótica de cadáver es un tejido de fácil acceso y con buenos resultados post-quirúrgicos por lo que no solo es usado en el ámbito de
la patología escleral, sino que también es utilizado para cirugía de oculoplástica, glaucoma o de retina.
No obstante, el peligro de transmisión de priones o el déficit de suministro en algunos países hacen que
este tejido sea suplantado por otros tejidos o técnicas quirúrgicas.
Métodos: Los objetivos de este estudio son identificar la patología esclerótica susceptible de terapia
sustitutiva en los últimos 5 años en el complejo hospitalario universitario de Granada, identificar los métodos empleados como terapéutica sustitutiva en dicha patología y evaluar la práctica de dicha terapia
en el contexto de la bibliografía existente al respecto. Para ello, se aplicó un diseño retrospectivo en el
que se realizó una búsqueda en los archivos de la unidad de documentación clínica del Hospital Clínico
San Cecilio y Hospital Virgen de las Nieves, de los cuales se obtuvieron 9 casos clínicos.
Resultados: Entre los 9 casos clínicos, solo 4 fueron sometidos a tratamiento quirúrgico, y de estos, 3
de ellos a terapia sustitutiva por membrana amniótica o injerto escleral. En los 5 casos restantes no se
llevó a cabo ninguna técnica quirúrgica, no obstante, podría ser de gran valor el uso del refuerzo con
injerto de esclerótica en los casos con escleromalacia para evitar complicaciones futuras. La esclerótica
procedente de cadáver se postula como una opción con unos resultados prometedores; sin embargo, las
líneas de investigación actuales apuestan por el desarrollo de un sustituto basado en las ventajas de la
fibrina agarosa que supla los puntos negativos del tejido donante.Introduction: The sclera is an avascular tissue composed of dense connective tissue whose main
function is to protect intraocular structures. There are several pathologies that jeopardize the
integrity of this tissue and, consequently, can cause terrible consequences with a dire prognosis.
Substitute treatment is part of the therapy of this pathology, with the donor sclera graft being a
biocompatible and biomimetic option to be evaluated. The donor sclera graft is an easily accessible
tissue with good post-surgical results, so it is not only used in the field of scleral pathology, but also
used for oculoplastic, glaucoma or retinal surgery. However, the danger of prion transmission or the
supply deficit in some countries causes that, other tissues or surgical techniques supplant this tissue.
Methods: The objectives of this study are to identify the sclerotic pathology susceptible of replacement
therapy in the last 5 years in the university hospital of Granada, identify the methods used as a
replacement therapy in this pathology and evaluate the practice of such therapy. A retrospective
design was applied and a search was made in the archives of the clinical documentation unit of the San Cecilio Clinical Hospital and Virgen de las Nieves Hospital. There were 9 clinical cases found.
Results: Among the nine clinical cases, only 4 were subjected to surgical treatment, and of these, three
of them for replacement therapy of donor sclera or amniotic membrane. In the remaining 5 cases did not
carry out any surgical technique, however, it could be of great value using reinforcement sclera graft in
cases with scleromalacia to avoid further complications. Sclera from cadaver is postulated as an option
with promising results; however, current research committed to the development of a substitute based
on the advantages of fibrin agarose that mitigates the negative points of the donor tissue
Counteracting gradients of light and soil nutrients in the understorey of Mediterranean oak forests.
The forest canopy modifies the availability of resources (light, water, and soil nutrients)
in the understorey. In this paper we analyze the relationships between woody canopy
density, litter accumulation, and topsoil N and P availability in the understorey of two
oak forests: one in southern Portugal and the other in southern Spain. Both forests
persist on low-nutrient soils, particularly poor in P. We hypothesize that direct and
indirect effects of the canopy overstorey cause opposite gradients in the availability of
essential resources (light and key soil nutrients) in the understorey. In both studied
forests we found significant relationships between the overall canopy density, light availability,
topsoil litter accumulation, and the availability of N and P, which frequently
limit plant growth. Path analysis (by Shipley’s d-sep method) showed that the available
data were consistent with the proposed causal model. The average values of soil variables
at the end quartiles of the light-availability gradient were compared. Results showed
large differences in litter accumulation (~30×) and available-N and -P topsoil concentrations
(~3×) in the Spanish forest (with the wider environmental gradient). Furthermore,
P increased from the ‘very low’ range to the ‘low’ or even the ‘optimum’ range of
availability (according to standard plant growth criteria), which suggests potential effects
on the growth of the understorey plant species. We conclude that the counteracting
gradients of the essential resources -light and nutrients- in the forest understorey
resulted from direct and indirect effects of the canopy overstorey, respectively. We suggest
that these counteracting effects of the woody canopy on essential resources of different
nature must be considered when interpreting the patterns of understorey plant populations
and communities.The spanish MEC (CGL2005-05830-C03-01-BOS, DINAMED project) and the Portuguese FCT(SFRH/BD/8322/2002 grant to SMM)supported the research.Peer reviewe
Observation of two new baryon resonances
Two structures are observed close to the kinematic threshold in the mass spectrum in a sample of proton-proton collision data, corresponding
to an integrated luminosity of 3.0 fb recorded by the LHCb experiment.
In the quark model, two baryonic resonances with quark content are
expected in this mass region: the spin-parity and
states, denoted and .
Interpreting the structures as these resonances, we measure the mass
differences and the width of the heavier state to be
MeV,
MeV,
MeV, where the first and second
uncertainties are statistical and systematic, respectively. The width of the
lighter state is consistent with zero, and we place an upper limit of
MeV at 95% confidence level. Relative
production rates of these states are also reported.Comment: 17 pages, 2 figure
Observation of associated production of a boson with a meson in the~forward region
A search for associated production of a boson with an open charm meson is
presented using a data sample, corresponding to an integrated luminosity of
of proton--proton collisions at a centre-of-mass energy
of 7\,TeV, collected by the LHCb experiment. %% Seven candidate events for
associated production of a boson with a meson and four candidate
events for a boson with a meson are observed with a combined
significance of 5.1standard deviations. The production cross-sections in the
forward region are measured to be where the first uncertainty is statistical and the
second systematic.Comment: 18 pages, 2 figure
Measurements of the , , meson and baryon lifetimes
Measurements of -hadron lifetimes are reported using collision data,
corresponding to an integrated luminosity of 1.0fb, collected by the
LHCb detector at a centre-of-mass energy of Tev. Using the exclusive decays
, , ,
and the average decay
times in these modes are measured to be = 0.004 0.003 ps, =
0.006 0.004 ps, = 0.013
0.005 ps, = 0.027
0.006 ps and = 0.011
0.005 ps, where the first uncertainty is statistical and the second is
systematic. These represent the most precise lifetime measurements in these
decay modes. In addition, ratios of these lifetimes, and the ratio of the
decay-width difference, , to the average width, , in
the system, , are
reported. All quantities are found to be consistent with Standard Model
expectations.Comment: 28 pages, 4 figures. Updated reference
Observation of an Excited Bc+ State
Using pp collision data corresponding to an integrated luminosity of 8.5 fb-1 recorded by the LHCb experiment at center-of-mass energies of s=7, 8, and 13 TeV, the observation of an excited Bc+ state in the Bc+π+π- invariant-mass spectrum is reported. The observed peak has a mass of 6841.2±0.6(stat)±0.1(syst)±0.8(Bc+) MeV/c2, where the last uncertainty is due to the limited knowledge of the Bc+ mass. It is consistent with expectations of the Bc∗(2S31)+ state reconstructed without the low-energy photon from the Bc∗(1S31)+→Bc+γ decay following Bc∗(2S31)+→Bc∗(1S31)+π+π-. A second state is seen with a global (local) statistical significance of 2.2σ (3.2σ) and a mass of 6872.1±1.3(stat)±0.1(syst)±0.8(Bc+) MeV/c2, and is consistent with the Bc(2S10)+ state. These mass measurements are the most precise to date
Measurement of the lifetime
Using a data set corresponding to an integrated luminosity of ,
collected by the LHCb experiment in collisions at centre-of-mass energies
of 7 and 8 TeV, the effective lifetime in the
decay mode, , is measured to be ps. Assuming
conservation, corresponds to the lifetime of the light
mass eigenstate. This is the first measurement of the effective
lifetime in this decay mode.Comment: All figures and tables, along with any supplementary material and
additional information, are available at
https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2016-017.htm
Precision measurement of violation in decays
The time-dependent asymmetry in decays is
measured using collision data, corresponding to an integrated luminosity
of fb, collected with the LHCb detector at centre-of-mass energies
of and TeV. In a sample of 96 000 decays, the
-violating phase is measured, as well as the decay widths
and of the light and heavy mass eigenstates of the
system. The values obtained are rad, ps, andps, where the first uncertainty is
statistical and the second systematic. These are the most precise single
measurements of those quantities to date. A combined analysis with decays gives rad. All
measurements are in agreement with the Standard Model predictions. For the
first time the phase is measured independently for each polarisation
state of the system and shows no evidence for polarisation
dependence.Comment: 6 figure
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