70 research outputs found
Crystallization of the C-terminal domain of the mouse brain cytosolic long-chain acyl-CoA thioesterase
The mammalian long-chain acyl-CoA thioesterase, the enzyme that catalyses the hydrolysis of acyl-CoAs to free fatty acids, contains two fused 4HBT (4-hydroxybenzoyl-CoA thioesterase) motifs. The C-terminal domain of the mouse long-chain acyl-CoA thioesterase (Acot7) has been expressed in bacteria and crystallized. The crystals were obtained by vapour diffusion using PEG 2000 MME as precipitant at pH 7.0 and 290 K. The crystals have the symmetry of space group R32 ( unit-cell parameters a = b = 136.83, c = 99.82 angstrom, gamma = 120 degrees). Two molecules are expected in the asymmetric unit. The crystals diffract to 2.4 angstrom resolution using the laboratory X-ray source and are suitable for crystal structure determination
Adolescents' perspectives on traditional, nontraditional, and direct political activities: The role of identity-processing styles and political beliefs
The study examined whether adolescentsâ tendency to employ informational, or normative identity-processing style predicts their perceived effectiveness of different political activities. Data were taken from the broader longitudinal study conducted in the Czech Republic, and included reports from 179 participants (Time 1 = age 17; Time 2 = age 19). Path analyses suggested that adolescents who sought information tended to perceive non-traditional political activity (e.g., in civic organizations) as effective, while participantsâ normative conformism predicted disbelief in direct activity (e.g., petitions). Perceived effectiveness of traditional activity (e.g., voting) reflected adolescentsâ actual political trust rather than their identity-processing styles. These results complement previous findings on the correlates of identity-processing styles and adolescentsâ political thinking
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