Comparative Analysis of <i>Phlebotomus argentipes</i> Vector of Leishmaniasis in India and Sri Lanka

Phlebotomus argentipes is the predominant sandfly vector of leishmaniasis in the Indian subcontinent. India and Sri Lanka primarily report visceral and cutaneous leishmaniasis caused by Leishmania donovani. We compared Ph. argentipes from two locations, focusing on its morphological, molecular, and salivary protein characteristics. Sandflies were captured using CDC light traps and cattle-baited net traps. Species identification and morphological comparisons were carried out using standard taxonomic keys. DNA extracted from 12 Sri Lankan sandfly samples was PCR-amplified and sequenced for the variable region of Cytochrome oxidase subunit I. Existing DNA sequences of India from GenBank were utilized for a phylogenetic analysis between Sri Lanka and India. Salivary protein profiles were studied using SDS-PAGE, Western blot, and electrospray ionization/LC/MS/MS. The morphological similarities observed between female Ph. argentipes from India and Sri Lanka suggest the presence of Ph. argentipes var. glaucus. A phylogenetic analysis showed genetic divergence between Ph. argentipes populations, but both shared a similar salivary protein profile. A common, strong 30 kDa immunogenic band comprised PagSP05, PagSP06, and PagSP17 proteins of Ph. argentipes. The similarity between the immunogenic salivary proteins suggests their potential use as common markers for vector exposure or immune response stimulants across regions. The use of multiple samples for each category of serum would improve the comprehensiveness of the immunogenic profiles obtained

Pesticide poisoning in the developing world - a minimum pesticides list

In parts of the developing world, pesticide poisoning causes more deaths than infectious diseases. Use of pesticides is poorly regulated and often dangerous; their easy availability also makes them a popular method of self-harm. In 1985, the UN Food and Agriculture Organisation (FAO) produced a voluntary code of conduct for the pesticide industry in an attempt to limit the harmful effects of pesticides. Unfortunately, a lack of adequate government resources in the developing world makes this code ineffective, and thousands of deaths continue today. WHO has recommended that access to highly toxic pesticides be restricted--where this has been done, suicide rates have fallen. Since an Essential Drugs List was established in 1977, use of a few essential drugs has rationalised drug use in many regions. An analogous Minimum Pesticides List would identify a restricted number of less dangerous pesticides to do specific tasks within an integrated pest management system. Use of safer pesticides should result in fewer deaths, just as the change from barbiturates to benzodiazepines has reduced the number of deaths from pharmaceutical self-poisoning

Nationalistic Authorship and Resistance in Northeastern Sri Lanka

Post-war Sri Lanka is defined by the logic of Sinhala-Buddhist nationalism, glorification and expansion of the military, and the exponential growth of state-corporate economic projects. This article examines the performative politics of the state in mass ritual discourse and spatial domination while acknowledging the various ways in which elements of the Northeastern Tamil community in Sri Lanka are mobilising as an activist community in the post-war period, including political agitation and emancipatory initiatives that respond to social justice issues, such as land grabs. Offering an analysis premised on the concept of performative politics, this article interrogates the process by which the state defines itself, while the Tamil community has used performative politics to communicate the unacceptability of the post-war performance of power. A framework of performative politics in post-war Sri Lanka, I argue, introduces a new grammar of politics more responsive to the nationalistic Sinhala-Buddhist settler-colonial tendencies of the state and to the polyvalent nature of Tamil resistance. On the international level, the state performatively complies with international demands for accountability and reconciliation while continuing to oppress the Tamil minority and undertake nationalistic authorship of post-war space