Toronto Alexithymia Scale: Adaptação da Versão Brasileira para Adultos de Baixa Escolaridade

For the purpose of studying Alexithymia in low-educated adults, we intend to adapt the Brazilian version of the Toronto Alexithymia Scale (TAS-26) and to verify its internal consistency. With that aim, we translated the original TAS-26 (English) to Portuguese, adopting a colloquial language, without content distortion. An exploratory qualitative study interviewed 50 women (38-65 years, educationCon objeto de estudiar la Alexitimia en adultos con bajo nivel de educación, buscamos adaptar la versión brasileña de la Escala de Alexitimia de Toronto (TAS-26) y comprobar su consistencia interna. Para esto, traducimos la TAS-26 original (inglés) al portugués, con la adopción de un lenguaje sencillo, sin cambiar el contenido. Una encuesta cualitativa entrevistó a 50 mujeres (38-65 años, escolaridadCom objetivo de estudar a alexitimia em indivíduos adultos de baixa escolaridade, decidimos adaptar a versão brasileira da Toronto Alexithymia Scale (TAS-26) e verificar sua consistência interna. Para isso, traduzimos a TAS-26 original (inglês) para o português, com linguagem coloquial, sem alteração de conteúdo. Em estudo exploratório qualitativo foram entrevistadas 50 mulheres (38-65 anos, escolaridad

Greater fruit selection following an appearance-based compared with a health-based health promotion poster

Background: This study investigated the impact of an appearance-based compared to a traditional health-based public health message for healthy eating. Methods: 166 British University students (41 male; aged 20.6±1.9 years) were randomized to view either an appearance-based (n=82) or a health-based (n=84) fruit promotion poster. Intentions to consume fruit and immediate fruit selection (laboratory observation) were assessed immediately after poster-viewing and subsequent self-report fruit consumption was assessed 3 days later. Results: Intentions to consume fruit were not predicted by poster type (β=0.03, p=0.74), but were associated with fruit-based liking, past consumption, attitudes, and social norms (smallest β=0.16, p=0.04). Immediate fruit selection was greater following the appearance-based compared to the health-based poster (β=-0.24, p<0.01), and this effect remained when controlling for participant characteristics (β=-0.21, p<0.01). Subsequent fruit consumption was greater following the appearance-based compared to the health-based poster (β=-0.22, p=0.03), but this effect became non-significant on consideration of participant characteristics (β=-0.15, p=0.13), and was instead associated with fruit-based liking and past consumption (smallest β=0.24, p=0.03). Conclusions: These findings demonstrate the clear value of an appearance-based compared to a health-based health promotion poster for increasing fruit selection. A distinction between outcome measures, and the value of a behavioural measure is also demonstrated

Rorschach Comprehensive System on the evaluation of psychodynamic psychotherapy

The proposal aimed to evaluate changes due to psychoanalytic psychotherapy on individuals assisted in a university clinic. Thus, 68 adult subjects, both genders, were evaluated before and after one year of psychotherapy. The Rorschach Comprehensive System protocols were analyzed according to their level of complexity, half on low [R↓+F↑] and half on high [R↑+F↓]. Significant statistical differences were found for (a) increase in low complexity group and decrease in 'high' complexity group on: R; EA; es; SumY; SumShd; FC+CF+C; CF+C; M; active; FD; H+(H)+Hd+(Hd); H pure, DEPI, (b) decrease in low and increase in high on Lambda and F pure. A confluence of these results was observed; the high complexity group showing less turbulence of their emotions, and the low complexity group displaying openness to experiences, even if of a more discomforting kind. The therapy proved to be beneficial to the individuals.A proposta foi avaliar mudanças decorrentes de psicoterapia psicanalítica em pessoas assistidas em um serviço universitário. Assim, 68 pessoas adultas, de ambos os sexos, foram avaliadas antes e depois de um ano de psicoterapia. Os protocolos de Rorschach Sistema Compreensivo foram analisados de acordo com seu grau de complexidade, metade em menor [R↓+F↑] e metade em maior [R↑+F↓]. Diferenças estatisticamente significantes foram encontradas com (a) aumento no grupo de menor complexidade e diminuição no grupo de maior complexidade em: R; EA; es; SumY; SumShd; FC+CF+C; CF+C; M; ativo; FD; H+(H)+Hd+(Hd); H puro, DEPI, (b) diminuição no menor e aumento no maior em Lambda e F puro. Observou-se confluência dos resultados, com o grupo de maior complexidade com menos turbulência em suas emoções e o de menor complexidade expressando abertura às experiências, mesmo se desconfortáveis. A terapia mostrou trazer benefícios a essas pessoas.Universidade Federal de São Paulo (UNIFESP)UNIFESPSciEL

Efecto de derivados de tetrahidroquinolinas sobre la homeostasis del Ca2+ intracelular en células de cáncer de mama (MCF-7) y su relación con la apoptosis.

Trabajo realizado por otros ocho autores.Tetrahydroquinoline derivatives are interesting structures exhibiting a wide range of biological activities, including antitumor effects. In this investigation, the effect of the synthesized tetrahydroquinolines JS-56 and JS-92 on apoptosis, intracellular Ca2+ concentration ([Ca2+]i), and the sarco(endo)plasmic reticulum Ca2+ -ATPase (SERCA) activity was determined on MCF-7 breast cancer cells. Colorimetric assays were used to assess MCF-7 cells viability and SERCA activity. Fura-2 and rhodamine 123 were used to measure the intracellular Ca2+ concentration and the mitochondrial electrochemical potential, respecively. TUNEL assay was used to analyze DNA fragmentation, while caspase activity and NF-κB-dependent gene expression were assessed by luminescence. In silico models were used for molecular docking analysis. These compounds increase intracellular Ca2+ concentration; the main contribution is the Ca2+ entry from the extracellular milieu. Both JS-56 and JS-92 inhibit the activity of SERCA and dissipate the mitochondrial electrochemical potential through processes dependent and independent of the Ca2+ uptake by this organelle. Furthermore, JS-56 and JS-92 generate cytotoxicity in MCF-7 cells. The effect of JS-92 is higher than JS-56. Both compounds activate caspases 7 and 9, cause DNA fragmentation, and potentiate the effect of phorbol 12-myristate-13-acetate on NF-κB-dependent gene expression. Molecular docking analysis suggests that both compounds have a high interaction for SERCA, similar to thapsigargin. Both tetrahydroquinoline derivatives induced cell death through a combination of apoptotic events, increase [Ca2+]i, and inhibit SERCA activity by direct interaction.Los derivados de tetrahidroquinolina son estructuras interesantes que exhiben una amplia gama de actividades biológicas, incluyendo efectos antitumorales. Se determinó el efecto de las tetrahidroquinolinas sintetizadas JS-56 y JS-92 sobre la apoptosis, concentración intracelular de Ca2+ ([Ca2+]i) y la actividad Ca2+-ATPasa del retículo sarco(endo)plásmico (SERCA) en células de cáncer de mama MCF-7. Se usaron ensayos olorimétricos para evaluar la viabilidad de las células MCF-7 y la actividad SERCA. Se emplearon Fura-2 y rodamina 123 para medir la concentración de Ca2+ intracelular y el potencial electroquímico mitocondrial, respectivamente. El ensayo TUNEL se utilizó para analizar la fragmentación del ADN, mientras que la actividad de caspasas y la expresión génica dependiente de NF-κB se evaluaron mediante luminiscencia. Modelos in silico permitieron el análisis del acoplamiento molecular. Estos compuestos aumentan la concentración de Ca2+ intracelular; la principal contribución es la entrada de Ca2+ desde el medio extracelular. Tanto JS-56 como JS-92 inhiben la actividad de SERCA y disipan el potencial electroquímico mitocondrial a través de procesos dependientes e independientes de la captación de Ca2+ por este orgánulo. Además, JS-56 y JS-92 generan citotoxicidad en células MCF-7. El efecto de JS-92 es mayor que JS-56. Ambos compuestos activan las caspasas 7 y 9, provocan la fragmentación del ADN y potencian el efecto del 12-miristato-13-acetato de forbol en la expresión génica dependiente de NF-κB. El análisis de acoplamiento molecular sugiere que ambos compuestos tienen una alta interacción con SERCA, similar a la tapsigargina. Ambos derivados de tetrahidroquinolina indujeron la muerte celular a través de una combinación de eventos apoptóticos, aumento de [Ca2+]i e inhibición de la actividad SERCA por interacción directa

Qualidade Formal do Rorschach Sistema Compreensivo e R-PAS: Amostra de Casos Psiquiátricos

A criação do Sistema de Avaliação de Desempenho do Rorschach (R-PAS) requer estudos que possibilitem o uso na população brasileira. A qualidade formal (FQ) é uma categoria imprescindível na clínica e na pesquisa. O objetivo deste trabalho foi comparar variáveis de FQ de protocolos de Rorschach de pacientes psiquiátricos codificados pelo Sistema Compreensivo (SC) e pelo R-PAS. A amostra foi de 206 protocolos de Rorschach de pacientes adultos em tratamento psiquiátrico, que foram avaliados também pela SCID-I e SCID-II. A maioria dos protocolos foi aplicada pelo SC e recodificados de acordo com o R-PAS. O coeficiente kappa foi calculado, e foram comparadas as médias dessas variáveis nos dois sistemas. Os resultados do kappa variaram de concordância quase perfeita até consistência substancial para todas as variáveis, no entanto, as estatísticas descritivas confirmaram que o R-PAS provoca mais codificação de FQ ordinária e, o SC, de FQ menos.La creación del Sistema de Evaluación del Desempeño de Rorschach (R-PAS) requiere estudios para su uso en la población brasileña. La calidad formal (FQ) es una categoría esencial en la clínica y la investigación. El objetivo del estudio fue comparar variables de FQ de protocolos de Rorschach codificados por el Sistema Comprehensivo y R-PAS. La muestra consistió de 206 protocolos de Rorschach de pacientes adultos en tratamiento psiquiátrico, que también fueron evaluados por la SCID-I y SCID-II. La mayoría de los protocolos fue aplicada en el SC y re-codificados de acuerdo con el R-PAS. Se calculó el coeficiente kappa y se comparó el promedio de estas variables en los dos sistemas. Los resultados de kappa se variaron desde concordancia casi perfecta hasta consistencia considerable para todas las variables, pero las estadísticas descriptivas confirmaron que el R-PAS provoca más codificación de FQ ordinaria y el SC de FQ menos.The creation of the Rorschach Performance Assessment System (R-PAS) requires research that allows its use in the Brazilian population. The Formal Quality (FQ) category is essential both for clinic and research. The aim of this study was to compare form quality variables in Rorschach protocols from psychiatric patients and ratings coded in the Comprehensive System (CS) and R-PAS. The sample comprised 206 Rorschach protocols from adult patients in psychiatric treatment, who were also assessed by SCID-I and SCID-II. Most protocols were administered in the CS and recoded according to the R-PAS. The kappa coefficient was calculated, and we compared the means of these variables in both systems. The kappa results varied from almost perfect to substantial consistency for all variables, however, the descriptive statistics confirmed that the R-PAS elicits more FQ Ordinary coding while the CS elicits more FQ minus coding

Inventário de moluscos do estuário do Rio Paraíba no nordeste do Brasil

Os ecossistemas costeiros do nordeste do Brasil têm uma importante biodiversidade de moluscos marinhos, a qual ainda é insuficientemente estudada. Este trabalho representa um inventário dos moluscos em duas localidades no estuário do Rio Paraíba. Moluscos foram coletados entre 2014 e 2016 na costa e área de restinga localizadas nas propriedades de Treze de Maio e Costinha de Santo Antônio. Este levantamento malacofaunístico identificou 12 famílias, 20 gêneros e 21 espécies de bivalves, 17 famílias, 19 gêneros e 20 espécies de gastrópodes e uma espécie de cefalópode. Os bivalves da família Veneridae Rafinesque, 1815 foram os mais representativos com um total de cinco espécies. Os gastrópodes da família Littorinidae Children, 1834 apresentaram a mais alta riqueza de espécies. As espécies mais abundantes foram: Neritina virginea (Linnaeus, 1758), Brachidontes exustus (Linnaeus, 1758), Crassostrea brasiliana (Lamarck, 1819), Cerithium atratum (Born, 1778), Anomalocardia brasiliana (Gmelin, 1791), Parvanachis obesa (C. B. Adams, 1845), Phrontis polygonata (Lamarck, 1822), Littoraria angulifera (Lamarck, 1822), L. flava (King, 1832), Tagelus plebeius (Lightfoot, 1786), Echinolittorina lineolata (d'Orbigny, 1840) e Iphigenia brasiliensis (Lamarck, 1818). Os resultados mostram que a área estudada tem uma considerável riqueza de espécies de Mollusca, necessitando de monitoramento ambiental, principalmente devido à importância econômica de algumas espécies para a população local.Coastal ecosystems of northeastern Brazil have important biodiversity with regard to marine mollusks, which are insufficiently studied. Here we provide an inventory of mollusks from two sites in the estuary of the Paraíba River. Mollusks were collected in 2014 and 2016 on the coast and sandbanks located on the properties of Treze de Maio and Costinha de Santo Antônio. The malacofaunal survey identified 12 families, 20 genera and 21 species of bivalves, 17 families, 19 genera and 20 species of gastropods and one species of cephalopod. Bivalves of the family Veneridae Rafinesque, 1815 were the most representative, with a total of five species. Gastropods of the family Littorinidae Children, 1834 had the greatest species richness. The most abundant species were: Neritina virginea (Linnaeus, 1758), Brachidontes exustus (Linnaeus, 1758), Crassostrea brasiliana (Lamarck, 1819), Cerithium atratum (Born, 1778), Anomalocardia brasiliana (Gmelin, 1791), Parvanachis obesa (C. B. Adams, 1845), Phrontis polygonata (Lamarck, 1822), Littoraria angulifera (Lamarck, 1822), L. flava (King, 1832), Tagelus plebeius (Lightfoot, 1786), Echinolittorina lineolata (d'Orbigny, 1840) and Iphigenia brasiliensis (Lamarck, 1818). The results show that the study area has considerable species richness of Mollusca, requiring environmental monitoring in the region mainly due to the economic importance of some species to the local population.Campina