Use of airplanes for loft training

The use of an aircraft for line oriented flight training (LOFT) is examined. Cockpit resource management (CRM) and LOFT training are compared. The advantages of the LOFT is a better cost effectiveness since there is no need to buy a simulator. The flight crew workload can be limited to a minimum

Initiative uses of aircraft for flight training

The use of the aircraft rather than a flight simulator as a training device is investigated. Particular attention is paid to the application of LOFT concepts to the aircraft in its home environment

PEMBERDAYAAN LITERASI DIGITAL GURU MELALUI PELATIHAN PENGGUNAAN AUGMENTED REALITY

Abstrak: Literasi digital merupakan kemampuan guru masa kini yang perlu diberdayakan melalui kegiatan pelatihan yang berkelanjutan. Kegiatan ini bertujuan untuk memberdayakan literasi digital guru melalui penggunaan Augmented Reality. Harapannya adalah tetjadi peningkatan literasi digital guru setelah kegiatan pelatihan. Kegiatan dilaksanakan di SMPN Fatumfaun dengan melibatkan 14 orang guru. Kegiatan dilakukan menjadi tiga tahapan utama yaitu tahapan persiapan, tahapan pelatihan dan pembimbingan serta tahapan evaluasi. Untuk mengetahui data literasi digital guru sebelum dan sesudah pelaksanaan kegiatan maka digunakan angket literasi digital yang berisi 10 pernyataan. Berdasarkan hasil evaluasi diketahui bahwa terjadi peningkatan literasi digital guru. Sebelum mengikuti kegiatan ini, nilai rata-rata literasi digital guru SMPN Fatumfaun adalah 63,14 yang berada pada kategori cukup baik. Setelah mengikuti kegiatan ini, nilai rata-rata literasi digital guru SMPN Fatumfaun adalah 76,43 yang berada pada kategori baik. Hal tersebut menunjukkan adanya peningkatan literasi digital guru sebesar 21,05 %.Abstract: Digital literacy is a skill of today's teachers that needs to be harnessed through continuous professional development. This activity aims to strengthen the digital literacy of teachers through the use of Augmented Reality. It is expected that the digital literacy of the teachers will increase after the training activities. This activity has been implemented in Fatumfaun Junior High School with the participation of 14 teachers. The activities are carried out in three main stages, namely the preparation stage, the training and mentoring stage and the evaluation stage. A digital literacy questionnaire with 10 statements was used to collect data on teachers' digital literacy before and after the implementation of the activity. Based on the evaluation results, it is known that there is an increase in teachers' digital literacy. Before participating in this activity, the average digital literacy score of Fatumfaun Junior High School teachers was 63.14, which was in the good enough category. After participating in this activity, the average digital literacy score of Fatumfaun Junior High School teachers was 76.43, which was in the good category. This shows an increase of 21.05% in the digital literacy of the teachers

Positive psychotic symptoms as a marker of clinical severity in a transdiagnostic sample of help-seeking adolescents.

The present study aimed to examine the association between the presence, number, and type of positive psychotic symptoms (PPS) and clinical severity in adolescent patients. Five hundred-six patients aged 11-17 years were assigned to either the noPPS (n = 341), the delusional beliefs only (del; n = 32), the hallucinations only (hall; n = 80), or the delusional beliefs and hallucinations (del&hall; n = 53) group. Generalized Structural Equation Modeling was applied to identify the best-fitting model representing clinical severity indicated by psychiatric diagnoses, depressivity, personality pathology, non-suicidal self-injury, suicide attempts, perceived stress, and psychosocial impairments, assessed by interviews and questionnaires. The groups were compared concerning the final model's factors. The final model consisted of three factors representing psychopathology and functional impairments, self-harming behavior, and perceived stress (BIC difference to reference model: 103.99). Participants with any PPS scored higher on all factors than the noPPS group (differences in SD: 0.49-1.48). Additionally, the del&hall group scored 1.31 SD higher on psychopathology and functional impairments than the hall group, and 1.16 SD higher on self-harming behavior compared to the del group. Finally, the hall group scored 0.84 SD higher on self-harming behavior than the del group, with no group differences in the other factors. In adolescent patients, the presence of PPS may represent a marker for a more severe form of mental disorder, with hallucinations being indicative of self-harming behavior. Early transdiagnostic assessment of PPS seems indicated as it may inform treatment in the context of clinical staging

Biofortification of selenium in black soldier fly (Hermetia illucens) prepupae reared on seaweed or selenium enriched substrates

Selenium (Se) is an essential trace element for livestock. The element can be supplemented to feed in inorganic or organic Se forms, where the chemical form in the diet affects the accumulation of Se in animal tissues. Insects are known to be natural bioaccumulators of different nutrients, but no studies have so far looked up on the capacity of insects to be biofortified with Se, as a potential future source for Se in feed. In this study, black soldier fly (BSF, Hermetia illucens) larvae were reared on three different substrates: (1) a control substrate (CTR diet) of plant-ingredients (Gainesville diet); (2) Ascophyllum nodosum diet (AN30%), with 30% substitution of the alfalfa meal with the brown algae; and (3) a Se diet, where the CTR diet was fortified with 0.3 mg/kg of Se. All experiments were carried out under dark condition, at 25 °C with 70% relative humidity for two weeks, and the final BSF prepupae were analysed for Se, metals and minerals, and Se species. The mean total Se content in the prepupae reared on Se dietary group was over five times higher compared to the CTR group, whereas lower Se levels were detected in AN30% dietary group. Se speciation analyses showed that organic selenomethionine (SeMet) was the major Se species present, both in CTR and AN30% group. For the Se group, SeMet was accounting for 54% of the total Se. The levels of SeMet were higher in the Se group compared to the CTR group, indicating that BSF prepupae are able to convert inorganic Se to organic Se. The overall results show that the substrates hereby studied affect the total Se and Se species, as well as the levels of other elements, in the BSF prepupae.publishedVersio

Identification of fragments binding to SARS-CoV-2 nsp10 reveals ligand-binding sites in conserved interfaces between nsp10 and nsp14/nsp16

Since the emergence of SARS-CoV-2 in 2019, Covid-19 has developed into a serious threat to our health, social and economic systems. Although vaccines have been developed in a tour-de-force and are now increasingly available, repurposing of existing drugs has been less successful. There is a clear need to develop new drugs against SARS-CoV-2 that can also be used against future coronavirus infections. Non-structural protein 10 (nsp10) is a conserved stimulator of two enzymes crucial for viral replication, nsp14 and nsp16, exhibiting exoribonuclease and methyltransferase activities. Interfering with RNA proofreading or RNA cap formation represents intervention strategies to inhibit replication. We applied fragment-based screening using nano differential scanning fluorometry and X-ray crystallography to identify ligands targeting SARS-CoV-2 nsp10. We identified four fragments located in two distinct sites: one can be modelled to where it would be located in the nsp14–nsp10 complex interface and the other in the nsp16–nsp10 complex interface. Microscale thermophoresis (MST) experiments were used to quantify fragment affinities for nsp10. Additionally, we showed by MST that the interaction by nsp14 and 10 is weak and thereby that complex formation could be disrupted by small molecules. The fragments will serve as starting points for the development of more potent analogues using fragment growing techniques and structure-based drug design

Arsenic speciation in low-trophic marine food chain – An arsenic exposure study on microalgae (Diacronema lutheri) and blue mussels (Mytilus edulis L.)

Microalgae and blue mussels are known to accumulate undesirable substances from the environment, including arsenic (As). Microalgae can biotransform inorganic As (iAs) to organoarsenic species, which can be transferred to blue mussels. Knowledge on As uptake, biotransformation, and trophic transfer is important with regards to feed and food safety since As species have varying toxicities. In the current work, experiments were conducted in two parts: (1) exposure of the microalgae Diacronema lutheri to 5 and 10 μg/L As(V) in seawater for 4 days, and (2) dietary As exposure where blue mussels (Mytilus edulis L.) were fed with D. lutheri exposed to 5 and 10 μg/L As(V), or by aquatic exposure to 5 μg/L As(V) in seawater, for a total of 25 days. The results showed that D. lutheri can take up As from seawater and transform it to methylated As species and arsenosugars (AsSug). However, exposure to 10 μg/L As(V) resulted in accumulation of iAs in D. lutheri and lower production of methylated As species, which may suggest that detoxification mechanisms were overwhelmed. Blue mussels exposed to As via the diet and seawater showed no accumulation of As. Use of linear mixed models revealed that the blue mussels were gradually losing As instead, which may be due to As concentration differences in the mussels’ natural environment and the experimental setup. Both D. lutheri and blue mussels contained notable proportions of simple methylated As species and AsSug. Arsenobetaine (AB) was not detected in D. lutheri but present in minor fraction in mussels. The findings suggest that low-trophic marine organisms mainly contain methylated As species and AsSug. The use of low-trophic marine organisms as feed ingredients requires further studies since AsSug are regarded as potentially toxic, which may introduce new risks to feed and food safety.publishedVersio