Knowledge, attitude and prescriptional practice about fixed dose combinations among medical practitioners in tertiary care hospital

Background: Fixed dose combination (FDC) are currently most prescribed and misused drug forms. Although fixed dose combinations are clinically better than single dosage forms, improper prescription and large amounts of irrational FDC flooding leads to unwanted financial burden and increased resistance. Therefore a study was carried out to know about the existing scenario about Knowledge Attitude and Practice of Fixed dose combinations among the resident doctors and devise some suggestions for improving the rationality. The objective of the study was to evaluate the knowledge, attitude and prescriptional practice regarding fixed dose combinations among resident doctors working in a Government Theni medical college, Theni.Methods: A cross-sectional descriptive study involving 100 resident doctors was conducted using a pre validated questionnaire containing 21 questions to evaluate the knowledge and prescriptional practice of Fixed Dose Combination among the study participants.Results: Ninety-five percent of the study participants were aware of the Fixed dose combination. Increased compliance and synergistic drug effect are the commonly cited advantages, whereas difficulty in dose adjustment and increased side effects were the commonly told disadvantages. Only 13% of the participants could name a single banned drug. Antibiotics (63%), NSAIDS (18%) were the commonly prescribed FDCs among study participants. Internet (58%), followed by textbook (42%) and colleagues (37%) were the common sources of knowledge about FDCs. Increasing awareness about Essential Medicines List (63%), regular CME (55%) and prior notification (50%) are some of the measures recommended by study participants to improve the awareness.Conclusions: Although the participants are aware of basic knowledge of fixed dose combinations are acceptable, prescribing FDC them often, many of the study participants lack knowledge about the rationality of these drugs. From our study, conducting regular CME’s about new Fixed dose combinations in the medical colleges and periodic notification of FDC’s are some of the suggested approaches to improve the rationality of prescription. Since Internet is listed as the most common source of knowledge about FDC, information about FDC can be sent through mails, text messages, which will play a part in improving the rationality of FDC

Antidiabetic Activity and Molecular Docking Analysis of Milky Mushroom (Calocybe indica) Grown on the Renewable Substrate

As part of the circular economy idea, rice straw without additives has been investigated as a way to turn agricultural waste into products with added value. The nutrients in the substrate, Calocybe indica yield, and biological effects were all calculated. In this investigation, the methanol extract was used. GCMS was examined to discover the necessary compounds present in fungi. A surplus of mushrooms was harvested, and it was discovered that the growth of mushrooms on the rice substrate took place in less than five days. At a humidity of 93%, biological efficiency was determined to be in the range of 54.5-130.9%. In vitro and molecular docking results for the antidiabetic showed good inhibitory properties. As a result, rice straw could be a productive and affordable growing medium for milky mushrooms

Biological and Clinical Relevance of microRNAs in Mitochondrial Diseases

Mitochondrial dysfunction arises from an inadequate number of mitochondria, an inability to provide necessary substrates to mitochondria, or a dysfunction in their electron transport and a denosine triphosphate synthesis machinery. Occurrences of mitochondrial dysfunction are due to genetic or environmental changes in the mitochondria or in the nuclear DNA that codes mitochondrial components. Currently, drug options are available, yet no treatment exists in sight of this disease and needs a new insight into molecular and signaling pathways for this disease. microRNAs (miRNAs) are small, endogenous, and noncoding RNAs function as a master regulator of gene expression. The evolution of miRNAs in the past two decades emerged as a key regulator of gene expression that controls physiological pathological cellular differentiation processes, and metabolic homeostasis such as development and cancer. It has been known that miRNAs are a potential biomarker in both communicable and noncommunicable diseases. But, in the case of mitochondrial dysfunction in miRNAs, the number of studies and investigations are comparatively less than those on other diseases and dysfunctions. In this review, we have elaborated the roles of miRNAs in the mitochondrial diseases and dysfunctions

Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst

Genomic DNA of Strychnos minor (Dennst) of sixteen geographical regions were amplified using thirteen primers. The analyses of products of Radom Amplified Polymorphic DNA (RAPD) revealed polymorphism among the samples. The polymorphic information content (PIC) was maximum for the primer of OPB-04 (0.40) followed by the primer OPB-20 (0.39). Clustering analysis by Jaccard’s coefficient index exhibited similarity matrix and distance matrix. The amplified products of the 13 primers collectively showed similarity index ranged from 0.654 to 0.986. The phylogenetic trees were constructed by UPGMA cluster analysis. Significant variations in the amplified genomic DNA suggest genetic variability of population but low inter-population genetic segregation. Keywords: Strychnos minor, Molecular fingerprinting, RAPD, Polymorphic Information Conten

Antidiabetic Activity and Molecular Docking Analysis of Milky Mushroom (

As part of the circular economy idea, rice straw without additives has been investigated as a way to turn agricultural waste into products with added value. The nutrients in the substrate, Calocybe indica yield, and biological effects were all calculated. In this investigation, the methanol extract was used. GCMS was examined to discover the necessary compounds present in fungi. A surplus of mushrooms was harvested, and it was discovered that the growth of mushrooms on the rice substrate took place in less than five days. At a humidity of 93%, biological efficiency was determined to be in the range of 54.5-130.9%. In vitro and molecular docking results for the antidiabetic showed good inhibitory properties. As a result, rice straw could be a productive and affordable growing medium for milky mushrooms