Olive oil calcium soaps and rumen protected methionine in the diet of lactating ewes: effect on milk quality

Eight Massese ewes were fed 4 diets with alfalfa hay as the forage (73% on the DM basis): 1) control diet (C); 2) diet C supplemented with olive oil calcium soaps, 50 g/d (L); 3) diet C supplemented with protected methionine, 5 g/d (M) or 4) plus both soaps and methionine (ML); the experimenthal design was a 4x4 Latin square with 2 replicates per diet. During the experimental periods, lasting one week each, the ewes were milked twice daily (8:00 a.m. and 7:00 p.m.). Milk yield was not affected by diet quality, but milk fat percentage and 6.5% fat corrected milk yield were higher in diets L, M and ML with respect to diet C (P<0.05). Milk protein content was depressed and blood urea increased following the Ca soap diet alone or with protected methionine. Diet M worsened (P lesser than 0.05) Rennet clotting time (r) and curd firmness after 30 minutes (A30). Saturated fatty acids C10:0, C12:0, C14:0 and C16:0 were depressed in milk fat with the Ca soap supple- mented diet, some of them significantly. C18:1 increased (P lesser than 0.05) with diet L only, whereas the association of Ca salts and methionine in diet ML significantly affected the linoleic acid and CLA content. It is concluded that the use of olive oil fatty acids as a protected fat source seems to improve the milk fatty acid char- acteristics towards a safer pattern, but the presence of this type of Ca salts in the diet appears to worsen the metabol- ic utilisation of amino acids

Polypodium phyllitidis L. (Polypodiaceaea): a fern with a high sucrose content

A partir del extracto metanólico de Polypodium phyllitidis L (Polypodiaceae) se obtuvo un precipitado sumamente abundante que se comprobó se trataba de sacarosa, lo que determinó su cuantificación por cromatografía gaseosa dado su posible valor económico.An abundant precipitate was obtained from the methanolic extract of Polypodium phyllitidis L. (Polypodiaceae), which was identified as sucrose and quantified by gas chromatography due to its possible economic irnportance.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Polypodium phyllitidis L. (Polypodiaceaea): a fern with a high sucrose content

A partir del extracto metanólico de Polypodium phyllitidis L (Polypodiaceae) se obtuvo un precipitado sumamente abundante que se comprobó se trataba de sacarosa, lo que determinó su cuantificación por cromatografía gaseosa dado su posible valor económico.An abundant precipitate was obtained from the methanolic extract of Polypodium phyllitidis L. (Polypodiaceae), which was identified as sucrose and quantified by gas chromatography due to its possible economic irnportance.Colegio de Farmacéuticos de la Provincia de Buenos Aire

A comparative kinetic study of thiamphenicol in pre-ruminant lambs and calves

Eight healthy Holstein-Friesian calves and 8 Massese lambs of either sex (10–15-days old) were used to evaluate the pharmacokinetics of thiamphenicol after intravenous (i.v.) and oral (p.o.) administration (30 mg/kg). Plasma concentrations of thiamphenicol were determined by high-performance liquid chromatography on blood samples collected over 24 h following treatment. Pharmacokinetic variables of the drug were calculated for both species and after both administration routes. After intravenous administration of thiamphenicol, a rapid distribution phase was followed by a slower elimination phase and, when thiamphenicol was administered p.o., the bioavailability was about 60% in both species. The higher volume of distribution and the longer biological elimination half-lives in pre-ruminant compared with adult animals indicate that thiamphenicol distributes widely into the extravascular compartment of pre-ruminants. Interspecies differences were observed in the kinetic behaviour of thiamphenicol with respect to peak plasma concentration (Cmax), time of peak plasma concentration (Tmax), elimination half-life ðT1=2Þ and total clearance ðClBÞ. In conclusion intravenous or oral administration of 30 mg/kg of thiamphenicol provides plasma concentrations higher than minimum effective concentrations inhibiting bacterial growth (MICs) against most pathogens in pre-ruminant lambs and calves