Malignant mixed Mullerian tumors of the uterus: histopathological evaluation of cell cycle and apoptotic regulatory proteins

<p>Abstract</p> <p>Aim</p> <p>The aim of our study was to evaluate survival outcomes in malignant mixed Mullerian tumors (MMMT) of the uterus with respect to the role of cell cycle and apoptotic regulatory proteins in the carcinomatous and sarcomatous components.</p> <p>Methods</p> <p>23 cases of uterine MMMT identified from the Saskatchewan Cancer Agency (1970-1999) were evaluated. Immunohistochemical expression of Bad, Mcl-1, bcl-x, bak, mdm2, bax, p16, p21, p53, p27, EMA, Bcl-2, Ki67 and PCNA was correlated with clinico-pathological data including survival outcomes.</p> <p>Results</p> <p>Histopathological examination confirmed malignant epithelial component with homologous (12 cases) and heterologous (11 cases) sarcomatous elements. P53 was strongly expressed (70-95%) in 15 cases and negative in 5 cases. The average survival in the p53+ve cases was 3.56 years as opposed to 8.94 years in p53-ve cases. Overexpression of p16 and Mcl-1 were observed in patients with longer survival outcomes (> 2 years). P16 and p21 were overexpressed in the carcinomatous and sarcomatous elements respectively. Cyclin-D1 was focally expressed only in the carcinomatous elements.</p> <p>Conclusions</p> <p>Our study supports that a) cell cycle and apoptotic regulatory protein dysregulation is an important pathway for tumorigenesis and b) p53 is an important immunoprognostic marker in MMMT of the uterus.</p

Curing of bisphenol A-aniline based benzoxazine using phenolic, amino and mercapto accelerators

The curing of bisphenol A-aniline based benzoxazine was studied applying different accelerators (4,4'-thiodiphenol, o-dianisidine, 2-mercaptobenzimidazole and 4-mercaptophenol) to initiate the catalytic ring-opening of benzoxazine. Possible pathways of benzoxazine ring-opening, polymerization and cross-linking without and with the addition of different accelerators are presented. The curing kinetics was investigated by model-free kinetic analysis of experimental data obtained by differential scanning calorimetry (DSC). The addition of different accelerators significantly reduced the onset temperature of curing in dynamic experiments. The effects of accelerators on the results of isothermal conversion prediction were studied and discussed in detail. Among the used accelerators, thiodiphenol showed the best accelerating efficiency and was consequently used in further studies, where its amount was varied. By low heating rate DSC analysis the catalytic ring-opening, thermally accelerated ring-opening and the diffusion-controlled steps were identified. The amount of added accelerator affected particularly the ring-opening and diffusion-controlled steps

Physicochemical Transformations within Ephemeral Streambeds Related to Sewage Effluent Releases

Project Completion Report, OWRT Project No. A-040-ARIZ / Agreement No. 14-31-0001-4003 / Project Dates: July, 1972 - June, 1974 / Acknowledgement - The work upon which this report is based was supported by funds provided by the United States Department of the Interior, Office of Water Research and Technology, as authorized under the Water Resources Research Act of 1964.Hydraulic and physicochemical measurements were made on treated sewage effluent releases at established locations within the channel of an ephemeral stream, the Santa Cruz River of Southern Arizona. Water quality samples were taken in sequence so that incremental flows at different hydrograph stages could be traced as the effluent moved downstream. Hydrographs obtained from two H -L flumes were used to calibrate a modified kinematic wave model. Hydraulic parameters from the kinematic model and physicochemical measurements from water quality samples were combined together into a statistical-empirical kinetic model of nitrogen transformations which may occur in sewage effluent releases. There was fair agreement between the measured data and the nitrogen species values calculated with the model. Measured nitrogen species values indicated that the rate of nitrification in sewage effluent releases is related to flow distance and physical characteristics of the stream.This item is part of the Water Resources Research Center collection. It was digitized from a physical copy provided by the Water Resources Research Center at The University of Arizona. For more information about items in this collection, please contact the Center, (520) 621-9591 or see http://wrrc.arizona.edu

Water Quality Transformations and Groundwater Recharge of Sewage Effluent Releases in an Ephemeral Stream Channel

Project Completion Report, OWRT Project No. A-051-ARIZ / Agreement No. 14-31-0001-5003 / Project Dates: July 1974 - June 1975 / Acknowledgement: The work upon which this report is based was supported by funds provided by the United States Department of the Interior, Office of Water Research and Technology, as authorized under the Water Resources Research Act of 1978.Bio-physicochemical measurements were made on treated sewage effluent releases at established locations within the channel of an ephemeral stream, the Santa Cruz River of Southern Arizona. Water samples were taken in chronological sequence as the effluent moved downstream, to trace changes in quality parameters during low and high hydrograph stages. Results indicate that dissolved oxygen (DO) concentrations at low effluent flows were higher than DO concentrations at high effluent flows; while, conversely, biochemical oxygen demand (BOD) concentrations at low effluent flows were generally lower than BOD concentrations at high effluent flows. Biochemical oxygen demand concentrations are affected by waste loadings, flow conditions, phytoplankton growth and nitrification. Mean river deoxygenation rates (k ) in sewage flows after six river miles from the Tucson Sewage Treatment Plant were always negative or increasing, indicative of nitrification, algal growth, and concentration of organic constituents through seepage losses.This item is part of the Water Resources Research Center collection. It was digitized from a physical copy provided by the Water Resources Research Center at The University of Arizona. For more information about items in this collection, please contact the Center, (520) 621-9591 or see http://wrrc.arizona.edu

Extended Use of Treated Municipal Wastewater by the Buckeye Irrigation Company: A Documentation of Effects

Project Completion Report, OWRT Project No. A-050-ARIZ. / Agreement No. 14-31-0001-5003 / Project Dates: July 1974 - June 1976. / The work upon which this publication was based was supported in part by funds provided by the Office of Water Research and Technology (A-050-ARIZ), U.S. Department of the Interior, Washington, D.C., as authorized by the Water Research and Development Act of 1978.INTRODUCTION: The use of treated sewage effluent by the Buckeye Irrigation Company began with 800 acre -feet in 1962 and had increased to 40,000 a.f. by 1968. The effluent was diverted by the Buckeye Irrigation Company from the Gila River approximately seven miles below the City of Phoenix 91st Avenue treatment plant, as it became available at their diversion point. Natural streamflow, used in earlier years, had virtually stopped due to upstream development except in heavy runoff years such as 1941. The ground water in the district of the Buckeye Irrigation Company is relatively high in dissolved solids. The quality of the treated effluent is better. In 1971 the company signed a 40 -year contract with Phoenix to assure its use of 30,000 a.f. of effluent per year. The effluent is mixed with native ground water to bring the total water applied on the 18,000-acre district up to approximately 90,000 a.f. (Halpenny, 1973). The treated effluent use by the Buckeye Irrigation Company is the largest in the State of Arizona and one of the largest land applications of treated effluent in the United States. It is unique in that it is being utilized by an irrigation district. Most other uses have been by city operated farms or private farms under a single ownership. In spite of its uniqueness the effects of effluent use by the Buckeye Irrigation District had not, prior to this research, been well documented. This documentation was made in order to improve the general knowledge needed to extend this type of use to other areas in the state and nation. "Widespread consideration and utilization of land application cannot be made until such time as adequate information concerning the technique involved is made available. The experience gained by those who have successfully utilized this wastewater management should be used... specific evaluation of established systems in the various climatic zones would appear to be more fruitful than new research installations for determining long term effects on soils, vegetation, ground water and the indigenous ecology..." (Sullivan, et al., 1973). During the two year research period most of the initial objectives were achieved. The original specific objectives were: 1. To identify changes during an extended period of application of treated wastewater to irrigated fields in: a. irrigation practices b. cropping patterns c. fertilization practices d. crop yield response and quality e. quality of irrigation water, and f. soil properties. 2. To make a preliminary evaluation of the effects of the use of effluent on ground-water conditions. 3. To make a preliminary evaluation of changes in water costs and farm profits.One copyrighted article, Commercial Production of Wheat Grain Irrigated with Municipal Waste Water and Pump Water, removed from Appendix A.This item is part of the Water Resources Research Center collection. It was digitized from a physical copy provided by the Water Resources Research Center at The University of Arizona. For more information about items in this collection, please contact the Center, (520) 621-9591 or see http://wrrc.arizona.edu